De novo indol-3-ylmethyl glucosinolate biosynthesis, and not long-distance transport, contributes to defence of Arabidopsis against powdery mildew

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De novo indol-3-ylmethyl glucosinolate biosynthesis, and not long-distance transport, contributes to defence of Arabidopsis against powdery mildew. / Hunziker, Pascal; Ghareeb, Hassan; Wagenknecht, Lena; Crocoll, Christoph; Halkier, Barbara Ann; Lipka, Volker; Schulz, Alexander.

In: Plant, Cell and Environment, Vol. 43, No. 6, 2020, p. 1571-1583.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hunziker, P, Ghareeb, H, Wagenknecht, L, Crocoll, C, Halkier, BA, Lipka, V & Schulz, A 2020, 'De novo indol-3-ylmethyl glucosinolate biosynthesis, and not long-distance transport, contributes to defence of Arabidopsis against powdery mildew', Plant, Cell and Environment, vol. 43, no. 6, pp. 1571-1583. https://doi.org/10.1111/pce.13766

APA

Hunziker, P., Ghareeb, H., Wagenknecht, L., Crocoll, C., Halkier, B. A., Lipka, V., & Schulz, A. (2020). De novo indol-3-ylmethyl glucosinolate biosynthesis, and not long-distance transport, contributes to defence of Arabidopsis against powdery mildew. Plant, Cell and Environment, 43(6), 1571-1583. https://doi.org/10.1111/pce.13766

Vancouver

Hunziker P, Ghareeb H, Wagenknecht L, Crocoll C, Halkier BA, Lipka V et al. De novo indol-3-ylmethyl glucosinolate biosynthesis, and not long-distance transport, contributes to defence of Arabidopsis against powdery mildew. Plant, Cell and Environment. 2020;43(6):1571-1583. https://doi.org/10.1111/pce.13766

Author

Hunziker, Pascal ; Ghareeb, Hassan ; Wagenknecht, Lena ; Crocoll, Christoph ; Halkier, Barbara Ann ; Lipka, Volker ; Schulz, Alexander. / De novo indol-3-ylmethyl glucosinolate biosynthesis, and not long-distance transport, contributes to defence of Arabidopsis against powdery mildew. In: Plant, Cell and Environment. 2020 ; Vol. 43, No. 6. pp. 1571-1583.

Bibtex

@article{fb441e96e5fe4ca4bce969d41d9f778e,
title = "De novo indol-3-ylmethyl glucosinolate biosynthesis, and not long-distance transport, contributes to defence of Arabidopsis against powdery mildew",
abstract = "Powdery mildew is a fungal disease that affects a wide range of plants and reduces crop yield worldwide. As obligate biotrophs, powdery mildew fungi manipulate living host cells to suppress defence responses and to obtain nutrients. Members of the plant order Brassicales produce indole glucosinolates that effectively protect them from attack by non-adapted fungi. Indol-3-ylmethyl glucosinolate is constitutively produced in the phloem and transported to epidermal cells for storage. Upon attack, indol-3-ylmethyl glucosinolate is activated by CYP81F2 to provide broad-spectrum defence against fungi. How de novo biosynthesis and transport contribute to defence of powdery mildew-attacked epidermal cells is unknown. Bioassays and glucosinolate analysis demonstrate that GTR glucosinolate transporters are not involved in antifungal defence. Using quantitative live-cell imaging of fluorophore-tagged markers, we show that accumulation of the glucosinolate biosynthetic enzymes CYP83B1 and SUR1 is induced in epidermal cells attacked by the non-adapted barley powdery mildew Blumeria graminis f.sp. hordei. By contrast, glucosinolate biosynthesis is attenuated during interaction with the virulent powdery mildew Golovinomyces orontii. Interestingly, SUR1 induction is delayed during the Golovinomyces orontii interaction. We conclude that epidermal de novo synthesis of indol-3-ylmethyl glucosinolate contributes to CYP81F2-mediated broad-spectrum antifungal resistance and that adapted powdery mildews may target this process.",
keywords = "Arabidopsis, epidermis, Glucosinolate, biosynthesis, powdery mildew, transport",
author = "Pascal Hunziker and Hassan Ghareeb and Lena Wagenknecht and Christoph Crocoll and Halkier, {Barbara Ann} and Volker Lipka and Alexander Schulz",
year = "2020",
doi = "10.1111/pce.13766",
language = "English",
volume = "43",
pages = "1571--1583",
journal = "Plant, Cell and Environment",
issn = "0140-7791",
publisher = "Wiley-Blackwell",
number = "6",

}

RIS

TY - JOUR

T1 - De novo indol-3-ylmethyl glucosinolate biosynthesis, and not long-distance transport, contributes to defence of Arabidopsis against powdery mildew

AU - Hunziker, Pascal

AU - Ghareeb, Hassan

AU - Wagenknecht, Lena

AU - Crocoll, Christoph

AU - Halkier, Barbara Ann

AU - Lipka, Volker

AU - Schulz, Alexander

PY - 2020

Y1 - 2020

N2 - Powdery mildew is a fungal disease that affects a wide range of plants and reduces crop yield worldwide. As obligate biotrophs, powdery mildew fungi manipulate living host cells to suppress defence responses and to obtain nutrients. Members of the plant order Brassicales produce indole glucosinolates that effectively protect them from attack by non-adapted fungi. Indol-3-ylmethyl glucosinolate is constitutively produced in the phloem and transported to epidermal cells for storage. Upon attack, indol-3-ylmethyl glucosinolate is activated by CYP81F2 to provide broad-spectrum defence against fungi. How de novo biosynthesis and transport contribute to defence of powdery mildew-attacked epidermal cells is unknown. Bioassays and glucosinolate analysis demonstrate that GTR glucosinolate transporters are not involved in antifungal defence. Using quantitative live-cell imaging of fluorophore-tagged markers, we show that accumulation of the glucosinolate biosynthetic enzymes CYP83B1 and SUR1 is induced in epidermal cells attacked by the non-adapted barley powdery mildew Blumeria graminis f.sp. hordei. By contrast, glucosinolate biosynthesis is attenuated during interaction with the virulent powdery mildew Golovinomyces orontii. Interestingly, SUR1 induction is delayed during the Golovinomyces orontii interaction. We conclude that epidermal de novo synthesis of indol-3-ylmethyl glucosinolate contributes to CYP81F2-mediated broad-spectrum antifungal resistance and that adapted powdery mildews may target this process.

AB - Powdery mildew is a fungal disease that affects a wide range of plants and reduces crop yield worldwide. As obligate biotrophs, powdery mildew fungi manipulate living host cells to suppress defence responses and to obtain nutrients. Members of the plant order Brassicales produce indole glucosinolates that effectively protect them from attack by non-adapted fungi. Indol-3-ylmethyl glucosinolate is constitutively produced in the phloem and transported to epidermal cells for storage. Upon attack, indol-3-ylmethyl glucosinolate is activated by CYP81F2 to provide broad-spectrum defence against fungi. How de novo biosynthesis and transport contribute to defence of powdery mildew-attacked epidermal cells is unknown. Bioassays and glucosinolate analysis demonstrate that GTR glucosinolate transporters are not involved in antifungal defence. Using quantitative live-cell imaging of fluorophore-tagged markers, we show that accumulation of the glucosinolate biosynthetic enzymes CYP83B1 and SUR1 is induced in epidermal cells attacked by the non-adapted barley powdery mildew Blumeria graminis f.sp. hordei. By contrast, glucosinolate biosynthesis is attenuated during interaction with the virulent powdery mildew Golovinomyces orontii. Interestingly, SUR1 induction is delayed during the Golovinomyces orontii interaction. We conclude that epidermal de novo synthesis of indol-3-ylmethyl glucosinolate contributes to CYP81F2-mediated broad-spectrum antifungal resistance and that adapted powdery mildews may target this process.

KW - Arabidopsis, epidermis

KW - Glucosinolate

KW - biosynthesis

KW - powdery mildew

KW - transport

U2 - 10.1111/pce.13766

DO - 10.1111/pce.13766

M3 - Journal article

C2 - 32275065

VL - 43

SP - 1571

EP - 1583

JO - Plant, Cell and Environment

JF - Plant, Cell and Environment

SN - 0140-7791

IS - 6

ER -

ID: 246349860