Chromatographic and electrophoretic methods for nanodisc purification and analysis

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Chromatographic and electrophoretic methods for nanodisc purification and analysis. / Justesen, Bo Højen; Günther-Pomorski, Thomas.

In: Reviews in Analytical Chemistry, Vol. 33, No. 3, 2014, p. 165-172.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Justesen, BH & Günther-Pomorski, T 2014, 'Chromatographic and electrophoretic methods for nanodisc purification and analysis', Reviews in Analytical Chemistry, vol. 33, no. 3, pp. 165-172. https://doi.org/10.1515/revac-2014-0014

APA

Justesen, B. H., & Günther-Pomorski, T. (2014). Chromatographic and electrophoretic methods for nanodisc purification and analysis. Reviews in Analytical Chemistry, 33(3), 165-172. https://doi.org/10.1515/revac-2014-0014

Vancouver

Justesen BH, Günther-Pomorski T. Chromatographic and electrophoretic methods for nanodisc purification and analysis. Reviews in Analytical Chemistry. 2014;33(3):165-172. https://doi.org/10.1515/revac-2014-0014

Author

Justesen, Bo Højen ; Günther-Pomorski, Thomas. / Chromatographic and electrophoretic methods for nanodisc purification and analysis. In: Reviews in Analytical Chemistry. 2014 ; Vol. 33, No. 3. pp. 165-172.

Bibtex

@article{5318c675dbb9463bb7934b02276f0c34,
title = "Chromatographic and electrophoretic methods for nanodisc purification and analysis",
abstract = "Soluble nanoscale lipid bilayers, termed nanodiscs, are widely used in science for studying the membrane-anchored and integral membrane protein complexes under defined experimental conditions. Although their formation occurs by a self-assembly process, nanodisc purification and the verification of proper reconstitution are still major challenges during the sample preparation. This review gives an overview of the methods used for purifying and analyzing nanodiscs and nanodisc-reconstituted membrane proteins, with an emphasis on the chromatographic and electrophoretic approaches.",
keywords = "chromatography, free flow electrophoresis, mass spectrometry, nanodiscs, native PAGE",
author = "Justesen, {Bo H{\o}jen} and Thomas G{\"u}nther-Pomorski",
year = "2014",
doi = "10.1515/revac-2014-0014",
language = "English",
volume = "33",
pages = "165--172",
journal = "Reviews in Analytical Chemistry",
issn = "0048-752X",
publisher = "De Gruyter",
number = "3",

}

RIS

TY - JOUR

T1 - Chromatographic and electrophoretic methods for nanodisc purification and analysis

AU - Justesen, Bo Højen

AU - Günther-Pomorski, Thomas

PY - 2014

Y1 - 2014

N2 - Soluble nanoscale lipid bilayers, termed nanodiscs, are widely used in science for studying the membrane-anchored and integral membrane protein complexes under defined experimental conditions. Although their formation occurs by a self-assembly process, nanodisc purification and the verification of proper reconstitution are still major challenges during the sample preparation. This review gives an overview of the methods used for purifying and analyzing nanodiscs and nanodisc-reconstituted membrane proteins, with an emphasis on the chromatographic and electrophoretic approaches.

AB - Soluble nanoscale lipid bilayers, termed nanodiscs, are widely used in science for studying the membrane-anchored and integral membrane protein complexes under defined experimental conditions. Although their formation occurs by a self-assembly process, nanodisc purification and the verification of proper reconstitution are still major challenges during the sample preparation. This review gives an overview of the methods used for purifying and analyzing nanodiscs and nanodisc-reconstituted membrane proteins, with an emphasis on the chromatographic and electrophoretic approaches.

KW - chromatography

KW - free flow electrophoresis

KW - mass spectrometry

KW - nanodiscs

KW - native PAGE

U2 - 10.1515/revac-2014-0014

DO - 10.1515/revac-2014-0014

M3 - Journal article

AN - SCOPUS:84907692278

VL - 33

SP - 165

EP - 172

JO - Reviews in Analytical Chemistry

JF - Reviews in Analytical Chemistry

SN - 0048-752X

IS - 3

ER -

ID: 130638322