Characterization of methylsulfinylalkyl glucosinolate specific polyclonal antibodies

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Characterization of methylsulfinylalkyl glucosinolate specific polyclonal antibodies. / Mirza, Nadia Muhammad Akram; Schulz, Alexander; Halkier, Barbara Ann.

In: Journal of Plant Biochemistry and Biotechnology, Vol. 25, No. 4, 2016, p. 433-436.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Mirza, NMA, Schulz, A & Halkier, BA 2016, 'Characterization of methylsulfinylalkyl glucosinolate specific polyclonal antibodies', Journal of Plant Biochemistry and Biotechnology, vol. 25, no. 4, pp. 433-436. https://doi.org/10.1007/s13562-015-0345-3

APA

Mirza, N. M. A., Schulz, A., & Halkier, B. A. (2016). Characterization of methylsulfinylalkyl glucosinolate specific polyclonal antibodies. Journal of Plant Biochemistry and Biotechnology, 25(4), 433-436. https://doi.org/10.1007/s13562-015-0345-3

Vancouver

Mirza NMA, Schulz A, Halkier BA. Characterization of methylsulfinylalkyl glucosinolate specific polyclonal antibodies. Journal of Plant Biochemistry and Biotechnology. 2016;25(4):433-436. https://doi.org/10.1007/s13562-015-0345-3

Author

Mirza, Nadia Muhammad Akram ; Schulz, Alexander ; Halkier, Barbara Ann. / Characterization of methylsulfinylalkyl glucosinolate specific polyclonal antibodies. In: Journal of Plant Biochemistry and Biotechnology. 2016 ; Vol. 25, No. 4. pp. 433-436.

Bibtex

@article{a7e731f3a4d742dc84adde7b3a8b861d,
title = "Characterization of methylsulfinylalkyl glucosinolate specific polyclonal antibodies",
abstract = "Antibodies towards small molecules, like plant specialized metabolites, are valuable tools for developing quantitative and qualitative analytical techniques. Glucosinolates are the specialized metabolites characteristic of the Brassicales order. Here we describe the characterization of polyclonal rabbit antibodies raised against the 4-methylsulfinylbutyl glucosinolate, glucoraphanin that is one of the major glucosinolates in the model plant Arabidopsis thaliana (hereafter Arabidopsis). Analysis of the cross-reactivity of the antibodies against a number of glucosinolates demonstrated that it was highly selective for methionine-derived aliphatic glucosinolates with a methyl-sulfinyl group in the side chain. Use of crude plant extracts from Arabidopsis mutants with different glucosinolate profiles showed that the antibodies recognized aliphatic glucosinolates in a plant extract and did not cross-react with other metabolites. These methylsulfinylalkyl glucosinolate specific antibodies have prospective use in multiple applications such as ELISA, co-immunoprecipitation and immunolocalization of glucosinolates.",
author = "Mirza, {Nadia Muhammad Akram} and Alexander Schulz and Halkier, {Barbara Ann}",
year = "2016",
doi = "10.1007/s13562-015-0345-3",
language = "English",
volume = "25",
pages = "433--436",
journal = "Journal of Plant Biochemistry and Biotechnology",
issn = "0971-7811",
publisher = "Springer (India) Private Ltd.",
number = "4",

}

RIS

TY - JOUR

T1 - Characterization of methylsulfinylalkyl glucosinolate specific polyclonal antibodies

AU - Mirza, Nadia Muhammad Akram

AU - Schulz, Alexander

AU - Halkier, Barbara Ann

PY - 2016

Y1 - 2016

N2 - Antibodies towards small molecules, like plant specialized metabolites, are valuable tools for developing quantitative and qualitative analytical techniques. Glucosinolates are the specialized metabolites characteristic of the Brassicales order. Here we describe the characterization of polyclonal rabbit antibodies raised against the 4-methylsulfinylbutyl glucosinolate, glucoraphanin that is one of the major glucosinolates in the model plant Arabidopsis thaliana (hereafter Arabidopsis). Analysis of the cross-reactivity of the antibodies against a number of glucosinolates demonstrated that it was highly selective for methionine-derived aliphatic glucosinolates with a methyl-sulfinyl group in the side chain. Use of crude plant extracts from Arabidopsis mutants with different glucosinolate profiles showed that the antibodies recognized aliphatic glucosinolates in a plant extract and did not cross-react with other metabolites. These methylsulfinylalkyl glucosinolate specific antibodies have prospective use in multiple applications such as ELISA, co-immunoprecipitation and immunolocalization of glucosinolates.

AB - Antibodies towards small molecules, like plant specialized metabolites, are valuable tools for developing quantitative and qualitative analytical techniques. Glucosinolates are the specialized metabolites characteristic of the Brassicales order. Here we describe the characterization of polyclonal rabbit antibodies raised against the 4-methylsulfinylbutyl glucosinolate, glucoraphanin that is one of the major glucosinolates in the model plant Arabidopsis thaliana (hereafter Arabidopsis). Analysis of the cross-reactivity of the antibodies against a number of glucosinolates demonstrated that it was highly selective for methionine-derived aliphatic glucosinolates with a methyl-sulfinyl group in the side chain. Use of crude plant extracts from Arabidopsis mutants with different glucosinolate profiles showed that the antibodies recognized aliphatic glucosinolates in a plant extract and did not cross-react with other metabolites. These methylsulfinylalkyl glucosinolate specific antibodies have prospective use in multiple applications such as ELISA, co-immunoprecipitation and immunolocalization of glucosinolates.

U2 - 10.1007/s13562-015-0345-3

DO - 10.1007/s13562-015-0345-3

M3 - Journal article

VL - 25

SP - 433

EP - 436

JO - Journal of Plant Biochemistry and Biotechnology

JF - Journal of Plant Biochemistry and Biotechnology

SN - 0971-7811

IS - 4

ER -

ID: 167092889