Cell wall staining with Trypan blue enables quantitative analysis of morphological changes in yeast cells
Research output: Contribution to journal › Journal article › Research › peer-review
Documents
- fmicb-06-00107
Final published version, 4.66 MB, PDF document
Yeast cells are protected by a cell wall that plays an important role in the exchange of substances with the environment. The cell wall structure is dynamic and can adapt to different physiological states or environmental conditions. For the investigation of morphological changes, selective staining with fluorescent dyes is a valuable tool. Furthermore, cell wall staining is used to facilitate sub-cellular localization experiments with fluorescently-labeled proteins and the detection of yeast cells in non-fungal host tissues. Here, we report staining of Saccharomyces cerevisiae cell wall with Trypan Blue, which emits strong red fluorescence upon binding to chitin and yeast glucan; thereby, it facilitates cell wall analysis by confocal and super-resolution microscopy. The staining pattern of Trypan Blue was similar to that of the widely used UV-excitable, blue fluorescent cell wall stain Calcofluor White. Trypan Blue staining facilitated quantification of cell size and cell wall volume when utilizing the optical sectioning capacity of a confocal microscope. This enabled the quantification of morphological changes during growth under anaerobic conditions and in the presence of chemicals, demonstrating the potential of this approach for morphological investigations or screening assays.
Original language | English |
---|---|
Article number | 107 |
Journal | Frontiers in Microbiology |
Volume | 6 |
Number of pages | 8 |
ISSN | 1664-302X |
DOIs | |
Publication status | Published - 2015 |
Links
- http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00107/abstract
Final published version
Number of downloads are based on statistics from Google Scholar and www.ku.dk
No data available
ID: 130838271