Candidate pathogenicity factor/effector proteins of "Candidatus Phytoplasma solani" modulate plant carbohydrate metabolism, accelerate the ascorbate–glutathione cycle, and induce autophagosomes
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Candidate pathogenicity factor/effector proteins of "Candidatus Phytoplasma solani" modulate plant carbohydrate metabolism, accelerate the ascorbate–glutathione cycle, and induce autophagosomes. / Dermastia, Marina; Tomaž, Špela; Strah, Rebeka; Lukan, Tjaša; Coll, Anna; Dušak, Barbara; Anžič, Barbara; Čepin, Timotej; Wienkoop, Stefanie; Kladnik, Aleš; Zagorščak, Maja; Riedle-Bauer, Monika; Schönhuber, Christina; Weckwerth, Wolfram; Gruden, Kristina; Roitsch, Thomas; Pompe Novak, Maruša; Brader, Günter.
In: Frontiers in Plant Science, Vol. 14, 1232367, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Candidate pathogenicity factor/effector proteins of "Candidatus Phytoplasma solani" modulate plant carbohydrate metabolism, accelerate the ascorbate–glutathione cycle, and induce autophagosomes
AU - Dermastia, Marina
AU - Tomaž, Špela
AU - Strah, Rebeka
AU - Lukan, Tjaša
AU - Coll, Anna
AU - Dušak, Barbara
AU - Anžič, Barbara
AU - Čepin, Timotej
AU - Wienkoop, Stefanie
AU - Kladnik, Aleš
AU - Zagorščak, Maja
AU - Riedle-Bauer, Monika
AU - Schönhuber, Christina
AU - Weckwerth, Wolfram
AU - Gruden, Kristina
AU - Roitsch, Thomas
AU - Pompe Novak, Maruša
AU - Brader, Günter
N1 - Publisher Copyright: Copyright © 2023 Dermastia, Tomaž, Strah, Lukan, Coll, Dušak, Anžič, Čepin, Wienkoop, Kladnik, Zagorščak, Riedle-Bauer, Schönhuber, Weckwerth, Gruden, Roitsch, Pompe Novak and Brader.
PY - 2023
Y1 - 2023
N2 - The pathogenicity of intracellular plant pathogenic bacteria is associated with the action of pathogenicity factors/effectors, but their physiological roles for most phytoplasma species, including ‘Candidiatus Phytoplasma solani’ are unknown. Six putative pathogenicity factors/effectors from six different strains of ‘Ca. P. solani’ were selected by bioinformatic analysis. The way in which they manipulate the host cellular machinery was elucidated by analyzing Nicotiana benthamiana leaves after Agrobacterium-mediated transient transformation with the pathogenicity factor/effector constructs using confocal microscopy, pull-down, and co-immunoprecipitation, and enzyme assays. Candidate pathogenicity factors/effectors were shown to modulate plant carbohydrate metabolism and the ascorbate–glutathione cycle and to induce autophagosomes. PoStoSP06, PoStoSP13, and PoStoSP28 were localized in the nucleus and cytosol. The most active effector in the processes studied was PoStoSP06. PoStoSP18 was associated with an increase in phosphoglucomutase activity, whereas PoStoSP28, previously annotated as an antigenic membrane protein StAMP, specifically interacted with phosphoglucomutase. PoStoSP04 induced only the ascorbate–glutathione cycle along with other pathogenicity factors/effectors. Candidate pathogenicity factors/effectors were involved in reprogramming host carbohydrate metabolism in favor of phytoplasma own growth and infection. They were specifically associated with three distinct metabolic pathways leading to fructose-6-phosphate as an input substrate for glycolysis. The possible significance of autophagosome induction by PoStoSP28 is discussed.
AB - The pathogenicity of intracellular plant pathogenic bacteria is associated with the action of pathogenicity factors/effectors, but their physiological roles for most phytoplasma species, including ‘Candidiatus Phytoplasma solani’ are unknown. Six putative pathogenicity factors/effectors from six different strains of ‘Ca. P. solani’ were selected by bioinformatic analysis. The way in which they manipulate the host cellular machinery was elucidated by analyzing Nicotiana benthamiana leaves after Agrobacterium-mediated transient transformation with the pathogenicity factor/effector constructs using confocal microscopy, pull-down, and co-immunoprecipitation, and enzyme assays. Candidate pathogenicity factors/effectors were shown to modulate plant carbohydrate metabolism and the ascorbate–glutathione cycle and to induce autophagosomes. PoStoSP06, PoStoSP13, and PoStoSP28 were localized in the nucleus and cytosol. The most active effector in the processes studied was PoStoSP06. PoStoSP18 was associated with an increase in phosphoglucomutase activity, whereas PoStoSP28, previously annotated as an antigenic membrane protein StAMP, specifically interacted with phosphoglucomutase. PoStoSP04 induced only the ascorbate–glutathione cycle along with other pathogenicity factors/effectors. Candidate pathogenicity factors/effectors were involved in reprogramming host carbohydrate metabolism in favor of phytoplasma own growth and infection. They were specifically associated with three distinct metabolic pathways leading to fructose-6-phosphate as an input substrate for glycolysis. The possible significance of autophagosome induction by PoStoSP28 is discussed.
KW - autophagosome
KW - effector
KW - glycolysis
KW - pathogenicity factor
KW - StAMP
KW - ‘Candidatus Phytoplasma solani’
U2 - 10.3389/fpls.2023.1232367
DO - 10.3389/fpls.2023.1232367
M3 - Journal article
C2 - 37662165
AN - SCOPUS:85169564860
VL - 14
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
M1 - 1232367
ER -
ID: 369253946