Analytical implications of different methods for preparing plant cell wall material

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Analytical implications of different methods for preparing plant cell wall material. / Fangel, Jonatan U.; Jones, Catherine Y.; Ulvskov, Peter; Harholt, Jesper; Willats, William G.T.

In: Carbohydrate Polymers, Vol. 261, 117866, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Fangel, JU, Jones, CY, Ulvskov, P, Harholt, J & Willats, WGT 2021, 'Analytical implications of different methods for preparing plant cell wall material', Carbohydrate Polymers, vol. 261, 117866. https://doi.org/10.1016/j.carbpol.2021.117866

APA

Fangel, J. U., Jones, C. Y., Ulvskov, P., Harholt, J., & Willats, W. G. T. (2021). Analytical implications of different methods for preparing plant cell wall material. Carbohydrate Polymers, 261, [117866]. https://doi.org/10.1016/j.carbpol.2021.117866

Vancouver

Fangel JU, Jones CY, Ulvskov P, Harholt J, Willats WGT. Analytical implications of different methods for preparing plant cell wall material. Carbohydrate Polymers. 2021;261. 117866. https://doi.org/10.1016/j.carbpol.2021.117866

Author

Fangel, Jonatan U. ; Jones, Catherine Y. ; Ulvskov, Peter ; Harholt, Jesper ; Willats, William G.T. / Analytical implications of different methods for preparing plant cell wall material. In: Carbohydrate Polymers. 2021 ; Vol. 261.

Bibtex

@article{1d1adbec6dc8467e8a109643ec1260e1,
title = "Analytical implications of different methods for preparing plant cell wall material",
abstract = "Almost all plant cells are surrounded by a wall constructed of co-extensive networks of polysaccharides and proteoglycans. The capability to analyse cell wall components is essential for both understanding their complex biology and to fully exploit their numerous practical applications. Several biochemical and immunological techniques are used to analyse cell walls and in almost all cases the first step is the preparation of an alcohol insoluble residue (AIR). There is significant variation in the protocols used for AIR preparation, which can have a notable impact on the downstream extractability and detection of cell wall components. To explore these effects, we have formally compared ten AIR preparation methods and analysed polysaccharides subsequently extracted using high-performance anion exchange chromatography (HPAEC-PAD) and Micro Array Polymer Profiling (MAPP). Our results reveal the impact that AIR preparation has on downstream detection of cell wall components and the need for optimisation and consistency when preparing AIR.",
keywords = "Alcohol Insoluble Residue (AIR), Micro Array Polymer Profiling (MAPP), Plant cell wall, Polysaccharide analysis",
author = "Fangel, {Jonatan U.} and Jones, {Catherine Y.} and Peter Ulvskov and Jesper Harholt and Willats, {William G.T.}",
year = "2021",
doi = "10.1016/j.carbpol.2021.117866",
language = "English",
volume = "261",
journal = "Carbohydrate Polymers",
issn = "0144-8617",
publisher = "Pergamon Press",

}

RIS

TY - JOUR

T1 - Analytical implications of different methods for preparing plant cell wall material

AU - Fangel, Jonatan U.

AU - Jones, Catherine Y.

AU - Ulvskov, Peter

AU - Harholt, Jesper

AU - Willats, William G.T.

PY - 2021

Y1 - 2021

N2 - Almost all plant cells are surrounded by a wall constructed of co-extensive networks of polysaccharides and proteoglycans. The capability to analyse cell wall components is essential for both understanding their complex biology and to fully exploit their numerous practical applications. Several biochemical and immunological techniques are used to analyse cell walls and in almost all cases the first step is the preparation of an alcohol insoluble residue (AIR). There is significant variation in the protocols used for AIR preparation, which can have a notable impact on the downstream extractability and detection of cell wall components. To explore these effects, we have formally compared ten AIR preparation methods and analysed polysaccharides subsequently extracted using high-performance anion exchange chromatography (HPAEC-PAD) and Micro Array Polymer Profiling (MAPP). Our results reveal the impact that AIR preparation has on downstream detection of cell wall components and the need for optimisation and consistency when preparing AIR.

AB - Almost all plant cells are surrounded by a wall constructed of co-extensive networks of polysaccharides and proteoglycans. The capability to analyse cell wall components is essential for both understanding their complex biology and to fully exploit their numerous practical applications. Several biochemical and immunological techniques are used to analyse cell walls and in almost all cases the first step is the preparation of an alcohol insoluble residue (AIR). There is significant variation in the protocols used for AIR preparation, which can have a notable impact on the downstream extractability and detection of cell wall components. To explore these effects, we have formally compared ten AIR preparation methods and analysed polysaccharides subsequently extracted using high-performance anion exchange chromatography (HPAEC-PAD) and Micro Array Polymer Profiling (MAPP). Our results reveal the impact that AIR preparation has on downstream detection of cell wall components and the need for optimisation and consistency when preparing AIR.

KW - Alcohol Insoluble Residue (AIR)

KW - Micro Array Polymer Profiling (MAPP)

KW - Plant cell wall

KW - Polysaccharide analysis

U2 - 10.1016/j.carbpol.2021.117866

DO - 10.1016/j.carbpol.2021.117866

M3 - Journal article

C2 - 33766354

AN - SCOPUS:85101954873

VL - 261

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

M1 - 117866

ER -

ID: 272645094