Transient expression of P-type ATPases in tobacco epidermal cells

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Transient expression of P-type ATPases in tobacco epidermal cells. / Pedas, Lisbeth Rosager; Palmgren, Michael Broberg; Lopez Marques, Rosa Laura.

P-Type ATPases: methods and protocols. ed. / Maike Bublitz. Vol. Part VI Springer, 2016. p. 383-393 (Methods in Molecular Biology, Vol. 1377).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Pedas, LR, Palmgren, MB & Lopez Marques, RL 2016, Transient expression of P-type ATPases in tobacco epidermal cells. in M Bublitz (ed.), P-Type ATPases: methods and protocols. vol. Part VI, Springer, Methods in Molecular Biology, vol. 1377, pp. 383-393. https://doi.org/10.1007/978-1-4939-3179-8_34

APA

Pedas, L. R., Palmgren, M. B., & Lopez Marques, R. L. (2016). Transient expression of P-type ATPases in tobacco epidermal cells. In M. Bublitz (Ed.), P-Type ATPases: methods and protocols (Vol. Part VI, pp. 383-393). Springer. Methods in Molecular Biology Vol. 1377 https://doi.org/10.1007/978-1-4939-3179-8_34

Vancouver

Pedas LR, Palmgren MB, Lopez Marques RL. Transient expression of P-type ATPases in tobacco epidermal cells. In Bublitz M, editor, P-Type ATPases: methods and protocols. Vol. Part VI. Springer. 2016. p. 383-393. (Methods in Molecular Biology, Vol. 1377). https://doi.org/10.1007/978-1-4939-3179-8_34

Author

Pedas, Lisbeth Rosager ; Palmgren, Michael Broberg ; Lopez Marques, Rosa Laura. / Transient expression of P-type ATPases in tobacco epidermal cells. P-Type ATPases: methods and protocols. editor / Maike Bublitz. Vol. Part VI Springer, 2016. pp. 383-393 (Methods in Molecular Biology, Vol. 1377).

Bibtex

@inbook{0b486f741b5a4d3aba27d5d7302f289e,
title = "Transient expression of P-type ATPases in tobacco epidermal cells",
abstract = "Transient expression in tobacco cells is a convenient method for several purposes such as analysis of protein-protein interactions and the subcellular localization of plant proteins. A suspension of Agrobacterium tumefaciens cells carrying the plasmid of interest is injected into the intracellular space between leaf epidermal cells, which results in DNA transfer from the bacteria to the plant and expression of the corresponding proteins. By injecting mixes of Agrobacterium strains, this system offers the possibility to co-express a number of target proteins simultaneously, thus allowing for example protein-protein interaction studies. In this chapter, we describe the procedure to transiently express P-type ATPases in tobacco epidermal cells, with focus on subcellular localization of the protein complexes formed by P4-ATPases and their β-subunits.",
keywords = "Adenosine Triphosphatases, Agrobacterium, Gene Expression, Genetic Engineering, Molecular Imaging, Plant Epidermis, Tobacco, Transformation, Genetic, Journal Article",
author = "Pedas, {Lisbeth Rosager} and Palmgren, {Michael Broberg} and {Lopez Marques}, {Rosa Laura}",
year = "2016",
doi = "10.1007/978-1-4939-3179-8_34",
language = "English",
isbn = "978-1-4939-3178-1",
volume = "Part VI",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "383--393",
editor = "Maike Bublitz",
booktitle = "P-Type ATPases",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - Transient expression of P-type ATPases in tobacco epidermal cells

AU - Pedas, Lisbeth Rosager

AU - Palmgren, Michael Broberg

AU - Lopez Marques, Rosa Laura

PY - 2016

Y1 - 2016

N2 - Transient expression in tobacco cells is a convenient method for several purposes such as analysis of protein-protein interactions and the subcellular localization of plant proteins. A suspension of Agrobacterium tumefaciens cells carrying the plasmid of interest is injected into the intracellular space between leaf epidermal cells, which results in DNA transfer from the bacteria to the plant and expression of the corresponding proteins. By injecting mixes of Agrobacterium strains, this system offers the possibility to co-express a number of target proteins simultaneously, thus allowing for example protein-protein interaction studies. In this chapter, we describe the procedure to transiently express P-type ATPases in tobacco epidermal cells, with focus on subcellular localization of the protein complexes formed by P4-ATPases and their β-subunits.

AB - Transient expression in tobacco cells is a convenient method for several purposes such as analysis of protein-protein interactions and the subcellular localization of plant proteins. A suspension of Agrobacterium tumefaciens cells carrying the plasmid of interest is injected into the intracellular space between leaf epidermal cells, which results in DNA transfer from the bacteria to the plant and expression of the corresponding proteins. By injecting mixes of Agrobacterium strains, this system offers the possibility to co-express a number of target proteins simultaneously, thus allowing for example protein-protein interaction studies. In this chapter, we describe the procedure to transiently express P-type ATPases in tobacco epidermal cells, with focus on subcellular localization of the protein complexes formed by P4-ATPases and their β-subunits.

KW - Adenosine Triphosphatases

KW - Agrobacterium

KW - Gene Expression

KW - Genetic Engineering

KW - Molecular Imaging

KW - Plant Epidermis

KW - Tobacco

KW - Transformation, Genetic

KW - Journal Article

U2 - 10.1007/978-1-4939-3179-8_34

DO - 10.1007/978-1-4939-3179-8_34

M3 - Book chapter

C2 - 26695049

SN - 978-1-4939-3178-1

VL - Part VI

T3 - Methods in Molecular Biology

SP - 383

EP - 393

BT - P-Type ATPases

A2 - Bublitz, Maike

PB - Springer

ER -

ID: 169883664