Transcriptional read-through of the long non-coding RNA SVALKA governs plant cold acclimation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Transcriptional read-through of the long non-coding RNA SVALKA governs plant cold acclimation. / Kindgren, Peter Robert; Ard, Ryan; Ivanov, Maxim; Marquardt, Sebastian.

In: Nature Communications, Vol. 9, 4561, 2018, p. 1-11.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kindgren, PR, Ard, R, Ivanov, M & Marquardt, S 2018, 'Transcriptional read-through of the long non-coding RNA SVALKA governs plant cold acclimation', Nature Communications, vol. 9, 4561, pp. 1-11. https://doi.org/10.1038/s41467-018-07010-6

APA

Kindgren, P. R., Ard, R., Ivanov, M., & Marquardt, S. (2018). Transcriptional read-through of the long non-coding RNA SVALKA governs plant cold acclimation. Nature Communications, 9, 1-11. [4561]. https://doi.org/10.1038/s41467-018-07010-6

Vancouver

Kindgren PR, Ard R, Ivanov M, Marquardt S. Transcriptional read-through of the long non-coding RNA SVALKA governs plant cold acclimation. Nature Communications. 2018;9:1-11. 4561. https://doi.org/10.1038/s41467-018-07010-6

Author

Kindgren, Peter Robert ; Ard, Ryan ; Ivanov, Maxim ; Marquardt, Sebastian. / Transcriptional read-through of the long non-coding RNA SVALKA governs plant cold acclimation. In: Nature Communications. 2018 ; Vol. 9. pp. 1-11.

Bibtex

@article{b86db5da60a54efc88e2646d1de76091,
title = "Transcriptional read-through of the long non-coding RNA SVALKA governs plant cold acclimation",
abstract = "Most DNA in the genomes of higher organisms does not encode proteins, yet much is transcribed by RNA polymerase II (RNAPII) into long non-coding RNAs (lncRNAs). The biological significance of most lncRNAs is largely unclear. Here, we identify a lncRNA (SVALKA) in a cold-sensitive region of the Arabidopsis genome. Mutations in SVALKA affect CBF1 expression and freezing tolerance. RNAPII read-through transcription of SVALKA results in a cryptic lncRNA overlapping CBF1 on the antisense strand, termed asCBF1. Our molecular dissection reveals that CBF1 is suppressed by RNAPII collision stemming from the SVALKA-asCBF1 lncRNA cascade. The SVALKA-asCBF1 cascade provides a mechanism to tightly control CBF1 expression and timing that could be exploited to maximize freezing tolerance with mitigated fitness costs. Our results provide a compelling example of local gene regulation by lncRNA transcription having a profound impact on the ability of plants to appropriately acclimate to challenging environmental conditions.",
author = "Kindgren, {Peter Robert} and Ryan Ard and Maxim Ivanov and Sebastian Marquardt",
note = "Author Correction: https://www.nature.com/articles/s41467-019-13269-0",
year = "2018",
doi = "10.1038/s41467-018-07010-6",
language = "English",
volume = "9",
pages = "1--11",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Transcriptional read-through of the long non-coding RNA SVALKA governs plant cold acclimation

AU - Kindgren, Peter Robert

AU - Ard, Ryan

AU - Ivanov, Maxim

AU - Marquardt, Sebastian

N1 - Author Correction: https://www.nature.com/articles/s41467-019-13269-0

PY - 2018

Y1 - 2018

N2 - Most DNA in the genomes of higher organisms does not encode proteins, yet much is transcribed by RNA polymerase II (RNAPII) into long non-coding RNAs (lncRNAs). The biological significance of most lncRNAs is largely unclear. Here, we identify a lncRNA (SVALKA) in a cold-sensitive region of the Arabidopsis genome. Mutations in SVALKA affect CBF1 expression and freezing tolerance. RNAPII read-through transcription of SVALKA results in a cryptic lncRNA overlapping CBF1 on the antisense strand, termed asCBF1. Our molecular dissection reveals that CBF1 is suppressed by RNAPII collision stemming from the SVALKA-asCBF1 lncRNA cascade. The SVALKA-asCBF1 cascade provides a mechanism to tightly control CBF1 expression and timing that could be exploited to maximize freezing tolerance with mitigated fitness costs. Our results provide a compelling example of local gene regulation by lncRNA transcription having a profound impact on the ability of plants to appropriately acclimate to challenging environmental conditions.

AB - Most DNA in the genomes of higher organisms does not encode proteins, yet much is transcribed by RNA polymerase II (RNAPII) into long non-coding RNAs (lncRNAs). The biological significance of most lncRNAs is largely unclear. Here, we identify a lncRNA (SVALKA) in a cold-sensitive region of the Arabidopsis genome. Mutations in SVALKA affect CBF1 expression and freezing tolerance. RNAPII read-through transcription of SVALKA results in a cryptic lncRNA overlapping CBF1 on the antisense strand, termed asCBF1. Our molecular dissection reveals that CBF1 is suppressed by RNAPII collision stemming from the SVALKA-asCBF1 lncRNA cascade. The SVALKA-asCBF1 cascade provides a mechanism to tightly control CBF1 expression and timing that could be exploited to maximize freezing tolerance with mitigated fitness costs. Our results provide a compelling example of local gene regulation by lncRNA transcription having a profound impact on the ability of plants to appropriately acclimate to challenging environmental conditions.

U2 - 10.1038/s41467-018-07010-6

DO - 10.1038/s41467-018-07010-6

M3 - Journal article

C2 - 30385760

VL - 9

SP - 1

EP - 11

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 4561

ER -

ID: 209116984