The Arabidopsis NPF3 protein is a GA transporter
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
The Arabidopsis NPF3 protein is a GA transporter. / Tal, Iris; Zhang, Yi; Jørgensen, Morten Egevang; Pisanty, Odelia; Barbosa, Inês C R; Zourelidou, Melina; Regnault, Thomas; Crocoll, Christoph; Olsen, Carl Erik; Weinstain, Roy; Schwechheimer, Claus; Halkier, Barbara Ann; Nour-Eldin, Hussam Hassan; Estelle, Mark; Shani, Eilon.
In: Nature Communications, Vol. 7, 11486, 2016.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - The Arabidopsis NPF3 protein is a GA transporter
AU - Tal, Iris
AU - Zhang, Yi
AU - Jørgensen, Morten Egevang
AU - Pisanty, Odelia
AU - Barbosa, Inês C R
AU - Zourelidou, Melina
AU - Regnault, Thomas
AU - Crocoll, Christoph
AU - Olsen, Carl Erik
AU - Weinstain, Roy
AU - Schwechheimer, Claus
AU - Halkier, Barbara Ann
AU - Nour-Eldin, Hussam Hassan
AU - Estelle, Mark
AU - Shani, Eilon
PY - 2016
Y1 - 2016
N2 - Gibberellins (GAs) are plant hormones that promote a wide range of developmental processes. While GA signalling is well understood, little is known about how GA is transported or how GA distribution is regulated. Here we utilize fluorescently labelled GAs (GA-Fl) to screen for Arabidopsis mutants deficient in GA transport. We show that the NPF3 transporter efficiently transports GA across cell membranes in vitro and GA-Fl in vivo. NPF3 is expressed in root endodermis and repressed by GA. NPF3 is targeted to the plasma membrane and subject to rapid BFA-dependent recycling. We show that abscisic acid (ABA), an antagonist of GA, is also transported by NPF3 in vitro. ABA promotes NPF3 expression and GA-Fl uptake in plants. On the basis of these results, we propose that GA distribution and activity in Arabidopsis is partly regulated by NPF3 acting as an influx carrier and that GA-ABA interaction may occur at the level of transport.
AB - Gibberellins (GAs) are plant hormones that promote a wide range of developmental processes. While GA signalling is well understood, little is known about how GA is transported or how GA distribution is regulated. Here we utilize fluorescently labelled GAs (GA-Fl) to screen for Arabidopsis mutants deficient in GA transport. We show that the NPF3 transporter efficiently transports GA across cell membranes in vitro and GA-Fl in vivo. NPF3 is expressed in root endodermis and repressed by GA. NPF3 is targeted to the plasma membrane and subject to rapid BFA-dependent recycling. We show that abscisic acid (ABA), an antagonist of GA, is also transported by NPF3 in vitro. ABA promotes NPF3 expression and GA-Fl uptake in plants. On the basis of these results, we propose that GA distribution and activity in Arabidopsis is partly regulated by NPF3 acting as an influx carrier and that GA-ABA interaction may occur at the level of transport.
U2 - 10.1038/ncomms11486
DO - 10.1038/ncomms11486
M3 - Journal article
C2 - 27139299
VL - 7
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
M1 - 11486
ER -
ID: 161192338