Synthesis and Purification of Lipid-conjugated Fluorescent pH Sensors

Research output: Contribution to journal › Journal article › Research › peer-review

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Synthesis and Purification of Lipid-conjugated Fluorescent pH Sensors. / Wiesner, Wiebke; Kühnel, Ronja Marie; Pomorski, Thomas Günther.

In: Bio-protocol, Vol. 13, No. 11, e4694, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Wiesner, W, Kühnel, RM & Pomorski, TG 2023, 'Synthesis and Purification of Lipid-conjugated Fluorescent pH Sensors', Bio-protocol, vol. 13, no. 11, e4694. https://doi.org/10.21769/BioProtoc.4694

APA

Wiesner, W., Kühnel, R. M., & Pomorski, T. G. (2023). Synthesis and Purification of Lipid-conjugated Fluorescent pH Sensors. Bio-protocol, 13(11), [e4694]. https://doi.org/10.21769/BioProtoc.4694

Vancouver

Wiesner W, Kühnel RM, Pomorski TG. Synthesis and Purification of Lipid-conjugated Fluorescent pH Sensors. Bio-protocol. 2023;13(11). e4694. https://doi.org/10.21769/BioProtoc.4694

Author

Wiesner, Wiebke ; Kühnel, Ronja Marie ; Pomorski, Thomas Günther. / Synthesis and Purification of Lipid-conjugated Fluorescent pH Sensors. In: Bio-protocol. 2023 ; Vol. 13, No. 11.

Bibtex

@article{42d08a78ca7e444ab28efc087359a88a,
title = "Synthesis and Purification of Lipid-conjugated Fluorescent pH Sensors",
abstract = "Lipid-conjugated pH sensors based on fluorophores coupled to lipids are a powerful tool for monitoring pH gradients in biological microcompartments and reconstituted membrane systems. This protocol describes the synthesis of pH sensors based on amine-reactive pHrodo esters and the amino phospholipid phosphatidylethanolamine. The major features of this sensor include efficient partitioning into membranes and strong fluorescence under acidic conditions. The protocol described here can be used as a template to couple other amine-reactive fluorophores to phosphatidylethanolamines.",
keywords = "Electrochemical gradients, Fluorescence, Labelled lipids, Liposome, Membrane transporter, pH sensor, Reconstitution",
author = "Wiebke Wiesner and K{\"u}hnel, {Ronja Marie} and Pomorski, {Thomas G{\"u}nther}",
note = "Publisher Copyright: {\textcopyright} 2023 The Authors.",
year = "2023",
doi = "10.21769/BioProtoc.4694",
language = "English",
volume = "13",
journal = "Bio-protocol",
issn = "2331-8325",
publisher = "bio-protocol",
number = "11",

}

RIS

TY - JOUR

T1 - Synthesis and Purification of Lipid-conjugated Fluorescent pH Sensors

AU - Wiesner, Wiebke

AU - Kühnel, Ronja Marie

AU - Pomorski, Thomas Günther

N1 - Publisher Copyright: © 2023 The Authors.

PY - 2023

Y1 - 2023

N2 - Lipid-conjugated pH sensors based on fluorophores coupled to lipids are a powerful tool for monitoring pH gradients in biological microcompartments and reconstituted membrane systems. This protocol describes the synthesis of pH sensors based on amine-reactive pHrodo esters and the amino phospholipid phosphatidylethanolamine. The major features of this sensor include efficient partitioning into membranes and strong fluorescence under acidic conditions. The protocol described here can be used as a template to couple other amine-reactive fluorophores to phosphatidylethanolamines.

AB - Lipid-conjugated pH sensors based on fluorophores coupled to lipids are a powerful tool for monitoring pH gradients in biological microcompartments and reconstituted membrane systems. This protocol describes the synthesis of pH sensors based on amine-reactive pHrodo esters and the amino phospholipid phosphatidylethanolamine. The major features of this sensor include efficient partitioning into membranes and strong fluorescence under acidic conditions. The protocol described here can be used as a template to couple other amine-reactive fluorophores to phosphatidylethanolamines.

KW - Electrochemical gradients

KW - Fluorescence

KW - Labelled lipids

KW - Liposome

KW - Membrane transporter

KW - pH sensor

KW - Reconstitution

U2 - 10.21769/BioProtoc.4694

DO - 10.21769/BioProtoc.4694

M3 - Journal article

C2 - 37342157

AN - SCOPUS:85164388315

VL - 13

JO - Bio-protocol

JF - Bio-protocol

SN - 2331-8325

IS - 11

M1 - e4694

ER -

ID: 372537343