Stabilization of dhurrin biosynthetic enzymes from Sorghum bicolor using a natural deep eutectic solvent
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Stabilization of dhurrin biosynthetic enzymes from Sorghum bicolor using a natural deep eutectic solvent. / Knudsen, Camilla; Bavishi, Krutika; Viborg, Ketil Mathiasen; Drew, Damian Paul; Simonsen, Henrik Toft; Motawia, Mohammed Saddik; Møller, Birger Lindberg; Laursen, Tomas.
In: Phytochemistry, Vol. 170, 112214, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Stabilization of dhurrin biosynthetic enzymes from Sorghum bicolor using a natural deep eutectic solvent
AU - Knudsen, Camilla
AU - Bavishi, Krutika
AU - Viborg, Ketil Mathiasen
AU - Drew, Damian Paul
AU - Simonsen, Henrik Toft
AU - Motawia, Mohammed Saddik
AU - Møller, Birger Lindberg
AU - Laursen, Tomas
PY - 2020
Y1 - 2020
N2 - In recent years, ionic liquids and deep eutectic solvents (DESs) have gained increasing attention due to their ability to extract and solubilize metabolites and biopolymers in quantities far beyond their solubility in oil and water. The hypothesis that naturally occurring metabolites are able to form a natural deep eutectic solvent (NADES), thereby constituting a third intracellular phase in addition to the aqueous and lipid phases, has prompted researchers to study the role of NADES in living systems. As an excellent solvent for specialized metabolites, formation of NADES in response to dehydration of plant cells could provide an appropriate environment for the functional storage of enzymes during drought. Using the enzymes catalyzing the biosynthesis of the defense compound dhurrin as an experimental model system, we demonstrate that enzymes involved in this pathway exhibit increased stability in NADES compared with aqueous buffer solutions, and that enzyme activity is restored upon rehydration. Inspired by nature, application of NADES provides a biotechnological approach for long-term storage of entire biosynthetic pathways including membrane-anchored enzymes.
AB - In recent years, ionic liquids and deep eutectic solvents (DESs) have gained increasing attention due to their ability to extract and solubilize metabolites and biopolymers in quantities far beyond their solubility in oil and water. The hypothesis that naturally occurring metabolites are able to form a natural deep eutectic solvent (NADES), thereby constituting a third intracellular phase in addition to the aqueous and lipid phases, has prompted researchers to study the role of NADES in living systems. As an excellent solvent for specialized metabolites, formation of NADES in response to dehydration of plant cells could provide an appropriate environment for the functional storage of enzymes during drought. Using the enzymes catalyzing the biosynthesis of the defense compound dhurrin as an experimental model system, we demonstrate that enzymes involved in this pathway exhibit increased stability in NADES compared with aqueous buffer solutions, and that enzyme activity is restored upon rehydration. Inspired by nature, application of NADES provides a biotechnological approach for long-term storage of entire biosynthetic pathways including membrane-anchored enzymes.
KW - Sorghum bicolor (L.) Moench
KW - Poaceae
KW - Natural deep eutectic solvents
KW - Metabolons
KW - Cytochromes P450
KW - Specialized metabolites
KW - Liquid-liquid phase separation
U2 - 10.1016/j.phytochem.2019.112214
DO - 10.1016/j.phytochem.2019.112214
M3 - Journal article
C2 - 31794881
VL - 170
JO - Phytochemistry
JF - Phytochemistry
SN - 0031-9422
M1 - 112214
ER -
ID: 231646189