Root-type ferredoxin-NADP+ oxidoreductase isoforms in Arabidopsis thaliana: Expression patterns, location and stress responses

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Root-type ferredoxin-NADP+ oxidoreductase isoforms in Arabidopsis thaliana : Expression patterns, location and stress responses. / Grabsztunowicz, Magda; Rantala, Marjaana; Ivanauskaite, Aiste; Blomster, Tiina; Koskela, Minna M.; Vuorinen, Katariina; Tyystjarvi, Esa; Burow, Meike; Overmyer, Kirk; Mahonen, Ari P.; Mulo, Paula.

In: Plant, Cell and Environment, Vol. 44, No. 2, 2021, p. 548-558.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Grabsztunowicz, M, Rantala, M, Ivanauskaite, A, Blomster, T, Koskela, MM, Vuorinen, K, Tyystjarvi, E, Burow, M, Overmyer, K, Mahonen, AP & Mulo, P 2021, 'Root-type ferredoxin-NADP+ oxidoreductase isoforms in Arabidopsis thaliana: Expression patterns, location and stress responses', Plant, Cell and Environment, vol. 44, no. 2, pp. 548-558. https://doi.org/10.1111/pce.13932

APA

Grabsztunowicz, M., Rantala, M., Ivanauskaite, A., Blomster, T., Koskela, M. M., Vuorinen, K., Tyystjarvi, E., Burow, M., Overmyer, K., Mahonen, A. P., & Mulo, P. (2021). Root-type ferredoxin-NADP+ oxidoreductase isoforms in Arabidopsis thaliana: Expression patterns, location and stress responses. Plant, Cell and Environment, 44(2), 548-558. https://doi.org/10.1111/pce.13932

Vancouver

Grabsztunowicz M, Rantala M, Ivanauskaite A, Blomster T, Koskela MM, Vuorinen K et al. Root-type ferredoxin-NADP+ oxidoreductase isoforms in Arabidopsis thaliana: Expression patterns, location and stress responses. Plant, Cell and Environment. 2021;44(2):548-558. https://doi.org/10.1111/pce.13932

Author

Grabsztunowicz, Magda ; Rantala, Marjaana ; Ivanauskaite, Aiste ; Blomster, Tiina ; Koskela, Minna M. ; Vuorinen, Katariina ; Tyystjarvi, Esa ; Burow, Meike ; Overmyer, Kirk ; Mahonen, Ari P. ; Mulo, Paula. / Root-type ferredoxin-NADP+ oxidoreductase isoforms in Arabidopsis thaliana : Expression patterns, location and stress responses. In: Plant, Cell and Environment. 2021 ; Vol. 44, No. 2. pp. 548-558.

Bibtex

@article{158bdf77311c43ec8cf016af7700fe2c,
title = "Root-type ferredoxin-NADP+ oxidoreductase isoforms in Arabidopsis thaliana: Expression patterns, location and stress responses",
abstract = "In Arabidopsis, two leaf-type ferredoxin-NADP(+) oxidoreductase (LFNR) isoforms function in photosynthetic electron flow in reduction of NADP(+), while two root-type FNR (RFNR) isoforms catalyse reduction of ferredoxin in non-photosynthetic plastids. As the key to understanding, the function of RFNRs might lie in their spatial and temporal distribution in different plant tissues and cell types, we examined expression of RFNR1 and RFNR2 genes using beta-glucuronidase (GUS) reporter lines and investigated accumulation of distinct RFNR isoforms using a GFP approach and Western blotting upon various stresses. We show that while RFNR1 promoter is active in leaf veins, root tips and in the stele of roots, RFNR2 promoter activity is present in leaf tips and root stele, epidermis and cortex. RFNR1 protein accumulates as a soluble protein within the plastids of root stele cells, while RFNR2 is mainly present in the outer root layers. Ozone treatment of plants enhanced accumulation of RFNR1, whereas low temperature treatment specifically affected RFNR2 accumulation in roots. We further discuss the physiological roles of RFNR1 and RFNR2 based on characterization of rfnr1 and rfnr2 knock-out plants and show that although the function of these proteins is partly redundant, the RFNR proteins are essential for plant development and survival.",
keywords = "Arabidopsis, ferredoxin&#8208, NADP(+) oxidoreductase, gene expression, low temperature, ozone, plastid, root, stress response, NADP+-OXIDOREDUCTASE, THYLAKOID MEMBRANES, GLUTAMATE SYNTHASE, NITRITE REDUCTION, ELECTRON-TRANSFER, DIFFERENT ORGANS, PLANTS, FNR, PURIFICATION, COMPLEXES",
author = "Magda Grabsztunowicz and Marjaana Rantala and Aiste Ivanauskaite and Tiina Blomster and Koskela, {Minna M.} and Katariina Vuorinen and Esa Tyystjarvi and Meike Burow and Kirk Overmyer and Mahonen, {Ari P.} and Paula Mulo",
year = "2021",
doi = "10.1111/pce.13932",
language = "English",
volume = "44",
pages = "548--558",
journal = "Plant, Cell and Environment",
issn = "0140-7791",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Root-type ferredoxin-NADP+ oxidoreductase isoforms in Arabidopsis thaliana

T2 - Expression patterns, location and stress responses

AU - Grabsztunowicz, Magda

AU - Rantala, Marjaana

AU - Ivanauskaite, Aiste

AU - Blomster, Tiina

AU - Koskela, Minna M.

AU - Vuorinen, Katariina

AU - Tyystjarvi, Esa

AU - Burow, Meike

AU - Overmyer, Kirk

AU - Mahonen, Ari P.

AU - Mulo, Paula

PY - 2021

Y1 - 2021

N2 - In Arabidopsis, two leaf-type ferredoxin-NADP(+) oxidoreductase (LFNR) isoforms function in photosynthetic electron flow in reduction of NADP(+), while two root-type FNR (RFNR) isoforms catalyse reduction of ferredoxin in non-photosynthetic plastids. As the key to understanding, the function of RFNRs might lie in their spatial and temporal distribution in different plant tissues and cell types, we examined expression of RFNR1 and RFNR2 genes using beta-glucuronidase (GUS) reporter lines and investigated accumulation of distinct RFNR isoforms using a GFP approach and Western blotting upon various stresses. We show that while RFNR1 promoter is active in leaf veins, root tips and in the stele of roots, RFNR2 promoter activity is present in leaf tips and root stele, epidermis and cortex. RFNR1 protein accumulates as a soluble protein within the plastids of root stele cells, while RFNR2 is mainly present in the outer root layers. Ozone treatment of plants enhanced accumulation of RFNR1, whereas low temperature treatment specifically affected RFNR2 accumulation in roots. We further discuss the physiological roles of RFNR1 and RFNR2 based on characterization of rfnr1 and rfnr2 knock-out plants and show that although the function of these proteins is partly redundant, the RFNR proteins are essential for plant development and survival.

AB - In Arabidopsis, two leaf-type ferredoxin-NADP(+) oxidoreductase (LFNR) isoforms function in photosynthetic electron flow in reduction of NADP(+), while two root-type FNR (RFNR) isoforms catalyse reduction of ferredoxin in non-photosynthetic plastids. As the key to understanding, the function of RFNRs might lie in their spatial and temporal distribution in different plant tissues and cell types, we examined expression of RFNR1 and RFNR2 genes using beta-glucuronidase (GUS) reporter lines and investigated accumulation of distinct RFNR isoforms using a GFP approach and Western blotting upon various stresses. We show that while RFNR1 promoter is active in leaf veins, root tips and in the stele of roots, RFNR2 promoter activity is present in leaf tips and root stele, epidermis and cortex. RFNR1 protein accumulates as a soluble protein within the plastids of root stele cells, while RFNR2 is mainly present in the outer root layers. Ozone treatment of plants enhanced accumulation of RFNR1, whereas low temperature treatment specifically affected RFNR2 accumulation in roots. We further discuss the physiological roles of RFNR1 and RFNR2 based on characterization of rfnr1 and rfnr2 knock-out plants and show that although the function of these proteins is partly redundant, the RFNR proteins are essential for plant development and survival.

KW - Arabidopsis

KW - ferredoxin&#8208

KW - NADP(+) oxidoreductase

KW - gene expression

KW - low temperature

KW - ozone

KW - plastid

KW - root

KW - stress response

KW - NADP+-OXIDOREDUCTASE

KW - THYLAKOID MEMBRANES

KW - GLUTAMATE SYNTHASE

KW - NITRITE REDUCTION

KW - ELECTRON-TRANSFER

KW - DIFFERENT ORGANS

KW - PLANTS

KW - FNR

KW - PURIFICATION

KW - COMPLEXES

U2 - 10.1111/pce.13932

DO - 10.1111/pce.13932

M3 - Journal article

C2 - 33131061

VL - 44

SP - 548

EP - 558

JO - Plant, Cell and Environment

JF - Plant, Cell and Environment

SN - 0140-7791

IS - 2

ER -

ID: 252470795