R-loops at microRNA encoding loci promote co-transcriptional processing of pri-miRNAs in plants
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
R-loops at microRNA encoding loci promote co-transcriptional processing of pri-miRNAs in plants. / Gonzalo, Lucia; Tossolini, Ileana; Gulanicz, Tomasz; Cambiagno, Damian A.; Kasprowicz-Maluski, Anna; Smolinski, Dariusz Jan; Mammarella, María Florencia; Ariel, Federico D.; Marquardt, Sebastian; Szweykowska-Kulinska, Zofia; Jarmolowski, Artur; Manavella, Pablo A.
In: Nature Plants, Vol. 8, No. 4, 2022, p. 402-418.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - R-loops at microRNA encoding loci promote co-transcriptional processing of pri-miRNAs in plants
AU - Gonzalo, Lucia
AU - Tossolini, Ileana
AU - Gulanicz, Tomasz
AU - Cambiagno, Damian A.
AU - Kasprowicz-Maluski, Anna
AU - Smolinski, Dariusz Jan
AU - Mammarella, María Florencia
AU - Ariel, Federico D.
AU - Marquardt, Sebastian
AU - Szweykowska-Kulinska, Zofia
AU - Jarmolowski, Artur
AU - Manavella, Pablo A.
N1 - Publisher Copyright: © 2022, The Author(s).
PY - 2022
Y1 - 2022
N2 - In most organisms, the maturation of nascent RNAs is coupled to transcription. Unlike in animals, the RNA polymerase II (RNAPII) transcribes microRNA genes (MIRNAs) as long and structurally variable pri-miRNAs in plants. Current evidence suggests that the miRNA biogenesis complex assembly initiates early during the transcription of pri-miRNAs in plants. However, it is unknown whether miRNA processing occurs co-transcriptionally. Here, we used native elongating transcript sequencing data and imaging techniques to demonstrate that plant miRNA biogenesis occurs coupled to transcription. We found that the entire biogenesis occurs co-transcriptionally for pri-miRNAs processed from the loop of the hairpin but requires a second nucleoplasmic step for those processed from the base. Furthermore, we found that co- and post-transcriptional miRNA processing mechanisms co-exist for most miRNAs in a dynamic balance. Notably, we discovered that R-loops, formed near the transcription start site region of MIRNAs, promote co-transcriptional pri-miRNA processing. Furthermore, our results suggest the neofunctionalization of co-transcriptionally processed miRNAs, boosting countless regulatory scenarios.
AB - In most organisms, the maturation of nascent RNAs is coupled to transcription. Unlike in animals, the RNA polymerase II (RNAPII) transcribes microRNA genes (MIRNAs) as long and structurally variable pri-miRNAs in plants. Current evidence suggests that the miRNA biogenesis complex assembly initiates early during the transcription of pri-miRNAs in plants. However, it is unknown whether miRNA processing occurs co-transcriptionally. Here, we used native elongating transcript sequencing data and imaging techniques to demonstrate that plant miRNA biogenesis occurs coupled to transcription. We found that the entire biogenesis occurs co-transcriptionally for pri-miRNAs processed from the loop of the hairpin but requires a second nucleoplasmic step for those processed from the base. Furthermore, we found that co- and post-transcriptional miRNA processing mechanisms co-exist for most miRNAs in a dynamic balance. Notably, we discovered that R-loops, formed near the transcription start site region of MIRNAs, promote co-transcriptional pri-miRNA processing. Furthermore, our results suggest the neofunctionalization of co-transcriptionally processed miRNAs, boosting countless regulatory scenarios.
U2 - 10.1038/s41477-022-01125-x
DO - 10.1038/s41477-022-01125-x
M3 - Journal article
C2 - 35449404
AN - SCOPUS:85128609985
VL - 8
SP - 402
EP - 418
JO - Nature Plants
JF - Nature Plants
SN - 2055-026X
IS - 4
ER -
ID: 306109479