Regulation and physiological function of proteins for heat tolerance in cowpea (Vigna unguiculata) genotypes under controlled and field conditions
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Regulation and physiological function of proteins for heat tolerance in cowpea (Vigna unguiculata) genotypes under controlled and field conditions. / Selinga, Tonny I.; Maseko, Sipho T.; Gabier, Hawwa; Rafudeen, Mohammed S.; Muasya, A. Muthama; Crespo, Olivier; Ogola, John B.O.; Valentine, Alex J.; Ottosen, Carl Otto; Rosenqvist, Eva; Chimphango, Samson B.M.
In: Frontiers in Plant Science, Vol. 13, 954527, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Regulation and physiological function of proteins for heat tolerance in cowpea (Vigna unguiculata) genotypes under controlled and field conditions
AU - Selinga, Tonny I.
AU - Maseko, Sipho T.
AU - Gabier, Hawwa
AU - Rafudeen, Mohammed S.
AU - Muasya, A. Muthama
AU - Crespo, Olivier
AU - Ogola, John B.O.
AU - Valentine, Alex J.
AU - Ottosen, Carl Otto
AU - Rosenqvist, Eva
AU - Chimphango, Samson B.M.
N1 - Publisher Copyright: Copyright © 2022 Selinga, Maseko, Gabier, Rafudeen, Muasya, Crespo, Ogola, Valentine, Ottosen, Rosenqvist and Chimphango.
PY - 2022
Y1 - 2022
N2 - The expression of heat shock proteins is considered a central adaptive mechanism to heat stress. This study investigated the expression of heat shock proteins (HSPs) and other stress-protective proteins against heat stress in cowpea genotypes under field (IT-96D-610 and IT-16) and controlled (IT-96D-610) conditions. Heat stress response analysis of proteins at 72 h in the controlled environment showed 270 differentially regulated proteins identified using label-free quantitative proteomics in IT-96D-610 plants. These plants expressed HSPs and chaperones [BAG family molecular chaperone 6 (BAG6), Multiprotein bridging factor1c (MBF1C) and cold shock domain protein 1 (CSDP1) in the controlled environment]. However, IT-96D-610 plants expressed a wider variety of small HSPs and more HSPs in the field. IT-96D-610 plants also responded to heat stress by exclusively expressing chaperones [DnaJ chaperones, universal stress protein and heat shock binding protein (HSBP)] and non-HSP proteins (Deg1, EGY3, ROS protective proteins, temperature-induced lipocalin and succinic dehydrogenase). Photosynthesis recovery and induction of proteins related to photosynthesis were better in IT-96D-610 because of the concurrent induction of heat stress response proteins for chaperone functions, protein degradation for repair and ROS scavenging proteins and PSII operating efficiency (Fq’/Fm′) than IT-16. This study contributes to identification of thermotolerance mechanisms in cowpea that can be useful in knowledge-based crop improvement.
AB - The expression of heat shock proteins is considered a central adaptive mechanism to heat stress. This study investigated the expression of heat shock proteins (HSPs) and other stress-protective proteins against heat stress in cowpea genotypes under field (IT-96D-610 and IT-16) and controlled (IT-96D-610) conditions. Heat stress response analysis of proteins at 72 h in the controlled environment showed 270 differentially regulated proteins identified using label-free quantitative proteomics in IT-96D-610 plants. These plants expressed HSPs and chaperones [BAG family molecular chaperone 6 (BAG6), Multiprotein bridging factor1c (MBF1C) and cold shock domain protein 1 (CSDP1) in the controlled environment]. However, IT-96D-610 plants expressed a wider variety of small HSPs and more HSPs in the field. IT-96D-610 plants also responded to heat stress by exclusively expressing chaperones [DnaJ chaperones, universal stress protein and heat shock binding protein (HSBP)] and non-HSP proteins (Deg1, EGY3, ROS protective proteins, temperature-induced lipocalin and succinic dehydrogenase). Photosynthesis recovery and induction of proteins related to photosynthesis were better in IT-96D-610 because of the concurrent induction of heat stress response proteins for chaperone functions, protein degradation for repair and ROS scavenging proteins and PSII operating efficiency (Fq’/Fm′) than IT-16. This study contributes to identification of thermotolerance mechanisms in cowpea that can be useful in knowledge-based crop improvement.
KW - cowpea
KW - heat shock proteins
KW - label-free protein quantification
KW - plant proteomics
KW - thermotolerance mechanisms
U2 - 10.3389/fpls.2022.954527
DO - 10.3389/fpls.2022.954527
M3 - Journal article
C2 - 36072323
AN - SCOPUS:85137752246
VL - 13
JO - Frontiers in Plant Science
JF - Frontiers in Plant Science
SN - 1664-462X
M1 - 954527
ER -
ID: 320875284