Psychoactive plant- and mushroom-associated alkaloids from two behavior modifying cicada pathogens
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Psychoactive plant- and mushroom-associated alkaloids from two behavior modifying cicada pathogens. / Boyce, Greg R.; Gluck-Thaler, Emile; Slot, Jason C.; Stajich, Jason E.; Davis, William J.; James, Tim Y.; Cooley, John R.; Panaccione, Daniel G.; Eilenberg, Jørgen; De Fine Licht, Henrik H.; Macias, Angie M.; Berger, Matthew C.; Wickert, Kristen L.; Stauder, Cameron M.; Spahr, Ellie J.; Maust, Matthew D.; Metheny, Amy M.; Simon, Chris; Kritsky, Gene; Hodge, Kathie T.; Humber, Richard A.; Gullion, Terry; Short, Dylan P.G.; Kijimoto, Teiya; Mozgai, Dan; Arguedas, Nidia; Kasson, Matt T.
In: Fungal Ecology, Vol. 41, 01.10.2019, p. 147-164.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Psychoactive plant- and mushroom-associated alkaloids from two behavior modifying cicada pathogens
AU - Boyce, Greg R.
AU - Gluck-Thaler, Emile
AU - Slot, Jason C.
AU - Stajich, Jason E.
AU - Davis, William J.
AU - James, Tim Y.
AU - Cooley, John R.
AU - Panaccione, Daniel G.
AU - Eilenberg, Jørgen
AU - De Fine Licht, Henrik H.
AU - Macias, Angie M.
AU - Berger, Matthew C.
AU - Wickert, Kristen L.
AU - Stauder, Cameron M.
AU - Spahr, Ellie J.
AU - Maust, Matthew D.
AU - Metheny, Amy M.
AU - Simon, Chris
AU - Kritsky, Gene
AU - Hodge, Kathie T.
AU - Humber, Richard A.
AU - Gullion, Terry
AU - Short, Dylan P.G.
AU - Kijimoto, Teiya
AU - Mozgai, Dan
AU - Arguedas, Nidia
AU - Kasson, Matt T.
PY - 2019/10/1
Y1 - 2019/10/1
N2 - Entomopathogenic fungi routinely kill their hosts before releasing infectious spores, but a few species keep insects alive while sporulating, which enhances dispersal. Transcriptomics- and metabolomics-based studies of entomopathogens with post-mortem dissemination from their parasitized hosts have unraveled infection processes and host responses. However, the mechanisms underlying active spore transmission by Entomophthoralean fungi in living insects remain elusive. Here we report the discovery, through metabolomics, of the plant-associated amphetamine, cathinone, in four Massospora cicadina-infected periodical cicada populations, and the mushroom-associated tryptamine, psilocybin, in annual cicadas infected with Massospora platypediae or Massospora levispora, which likely represent a single fungal species. The absence of some fungal enzymes necessary for cathinone and psilocybin biosynthesis along with the inability to detect intermediate metabolites or gene orthologs are consistent with possibly novel biosynthesis pathways in Massospora. The neurogenic activities of these compounds suggest the extended phenotype of Massospora that modifies cicada behavior to maximize dissemination is chemically-induced.
AB - Entomopathogenic fungi routinely kill their hosts before releasing infectious spores, but a few species keep insects alive while sporulating, which enhances dispersal. Transcriptomics- and metabolomics-based studies of entomopathogens with post-mortem dissemination from their parasitized hosts have unraveled infection processes and host responses. However, the mechanisms underlying active spore transmission by Entomophthoralean fungi in living insects remain elusive. Here we report the discovery, through metabolomics, of the plant-associated amphetamine, cathinone, in four Massospora cicadina-infected periodical cicada populations, and the mushroom-associated tryptamine, psilocybin, in annual cicadas infected with Massospora platypediae or Massospora levispora, which likely represent a single fungal species. The absence of some fungal enzymes necessary for cathinone and psilocybin biosynthesis along with the inability to detect intermediate metabolites or gene orthologs are consistent with possibly novel biosynthesis pathways in Massospora. The neurogenic activities of these compounds suggest the extended phenotype of Massospora that modifies cicada behavior to maximize dissemination is chemically-induced.
KW - Amphetamine
KW - Cathinone
KW - Entomopathogen
KW - Entomophthorales
KW - Invertebrate pathology
KW - Magicicada
KW - Massospora
KW - Okanagana
KW - Platypedia
KW - Psilocin
KW - Psilocybin
KW - Tryptamine
KW - Zoopagomycota
UR - http://www.scopus.com/inward/record.url?scp=85067621985&partnerID=8YFLogxK
U2 - 10.1016/j.funeco.2019.06.002
DO - 10.1016/j.funeco.2019.06.002
M3 - Journal article
C2 - 31768192
AN - SCOPUS:85067621985
VL - 41
SP - 147
EP - 164
JO - Fungal Ecology
JF - Fungal Ecology
SN - 1754-5048
ER -
ID: 223675732