TY - JOUR

T1 - Prevalence of antibiotic resistance genes in Pangasianodon hypophthalmus and Oreochromis niloticus aquaculture production systems in Bangladesh

AU - Lassen, Simon Bo

AU - Ahsan, Md Emranul

AU - Islam, Seikh Razibul

AU - Zhou, Xin Yuan

AU - Razzak, Muhammad Abdur

AU - Su, Jian Qiang

AU - Brandt, Kristian Koefoed

N1 - Publisher Copyright: © 2021 The Authors

PY - 2022

Y1 - 2022

N2 - Antibiotic resistance genes (ARGs) constitute emerging pollutants of significant public health concern. Antibiotics applied in aquaculture may stimulate the proliferation and dissemination of ARGs. This study investigated the prevalence and diversity of ARGs in Pangasianodon hypophthalmus (formerly Pangasius) and Oreochromis niloticus (formerly Tilapia) commercial aquaculture ponds from four economically important divisions (i.e. regions) of Bangladesh using a high-throughput qPCR ARG SmartChip and further aimed to explore effects of aquaculture pond management and water quality on the observed ARG prevalence patterns. A total of 160 ARGs and 10 mobile genetic elements (MGEs) were detected across all samples (n = 33), of which 76 ARGs and MGEs were shared between all regions. Multidrug resistance genes were the most frequently encountered ARGs, followed by ARGs conferring resistance to β-lactams, aminoglycosides, tetracyclines, and macrolide-lincosamide-streptogramin B (MLSB). Research ponds managed by the Bangladesh Agricultural University had the lowest abundance and diversity of ARGs, suggesting that proper management such as regular water quality monitoring, fortnightly water exchange and use of probiotics instead of antibiotics may mitigate the dissemination of antibiotic resistance from aquaculture ponds. The Adonis test (R2 = 0.35, p < 0.001) and distance decay relationships revealed that the ARGs composition displayed a significant biogeographical pattern (i.e., separation based on geographic origin). However, this effect could possibly be due to feed type as different feed types were used in different regions. In conclusion, our results indicate that there is a vast potential for improving aquaculture pond management practices in Bangladesh to mitigate the environmental dissemination of ARGs and their subsequent transmission to humans.

AB - Antibiotic resistance genes (ARGs) constitute emerging pollutants of significant public health concern. Antibiotics applied in aquaculture may stimulate the proliferation and dissemination of ARGs. This study investigated the prevalence and diversity of ARGs in Pangasianodon hypophthalmus (formerly Pangasius) and Oreochromis niloticus (formerly Tilapia) commercial aquaculture ponds from four economically important divisions (i.e. regions) of Bangladesh using a high-throughput qPCR ARG SmartChip and further aimed to explore effects of aquaculture pond management and water quality on the observed ARG prevalence patterns. A total of 160 ARGs and 10 mobile genetic elements (MGEs) were detected across all samples (n = 33), of which 76 ARGs and MGEs were shared between all regions. Multidrug resistance genes were the most frequently encountered ARGs, followed by ARGs conferring resistance to β-lactams, aminoglycosides, tetracyclines, and macrolide-lincosamide-streptogramin B (MLSB). Research ponds managed by the Bangladesh Agricultural University had the lowest abundance and diversity of ARGs, suggesting that proper management such as regular water quality monitoring, fortnightly water exchange and use of probiotics instead of antibiotics may mitigate the dissemination of antibiotic resistance from aquaculture ponds. The Adonis test (R2 = 0.35, p < 0.001) and distance decay relationships revealed that the ARGs composition displayed a significant biogeographical pattern (i.e., separation based on geographic origin). However, this effect could possibly be due to feed type as different feed types were used in different regions. In conclusion, our results indicate that there is a vast potential for improving aquaculture pond management practices in Bangladesh to mitigate the environmental dissemination of ARGs and their subsequent transmission to humans.

KW - Antibiotic resistance

KW - Aquaculture

KW - HT-qPCR

KW - Public health

KW - Water quality

U2 - 10.1016/j.scitotenv.2021.151915

DO - 10.1016/j.scitotenv.2021.151915

M3 - Journal article

C2 - 34826462

AN - SCOPUS:85120808347

VL - 813

JO - Science of the Total Environment

JF - Science of the Total Environment

SN - 0048-9697

M1 - 151915

ER -