Origin and evolution of transporter substrate specificity within the NPF family: [with Correction]

Research output: Contribution to journal › Journal article › Research › peer-review

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Origin and evolution of transporter substrate specificity within the NPF family : [with Correction]. / Jørgensen, Morten Egevang; Xu, Deyang; Crocoll, Christoph; Ernst, Heidi Asschenfeldt; Ramírez, David; Motawie, Mohammed Saddik; Olsen, Carl Erik; Mirza, Osman Asghar; Nour-Eldin, Hussam Hassan; Halkier, Barbara Ann.

In: eLife, Vol. 6, e19466, 2017.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jørgensen, ME, Xu, D, Crocoll, C, Ernst, HA, Ramírez, D, Motawie, MS, Olsen, CE, Mirza, OA, Nour-Eldin, HH & Halkier, BA 2017, 'Origin and evolution of transporter substrate specificity within the NPF family: [with Correction]', eLife, vol. 6, e19466. https://doi.org/10.7554/eLife.19466

APA

Jørgensen, M. E., Xu, D., Crocoll, C., Ernst, H. A., Ramírez, D., Motawie, M. S., Olsen, C. E., Mirza, O. A., Nour-Eldin, H. H., & Halkier, B. A. (2017). Origin and evolution of transporter substrate specificity within the NPF family: [with Correction]. eLife, 6, [e19466]. https://doi.org/10.7554/eLife.19466

Vancouver

Jørgensen ME, Xu D, Crocoll C, Ernst HA, Ramírez D, Motawie MS et al. Origin and evolution of transporter substrate specificity within the NPF family: [with Correction]. eLife. 2017;6. e19466. https://doi.org/10.7554/eLife.19466

Author

Jørgensen, Morten Egevang ; Xu, Deyang ; Crocoll, Christoph ; Ernst, Heidi Asschenfeldt ; Ramírez, David ; Motawie, Mohammed Saddik ; Olsen, Carl Erik ; Mirza, Osman Asghar ; Nour-Eldin, Hussam Hassan ; Halkier, Barbara Ann. / Origin and evolution of transporter substrate specificity within the NPF family : [with Correction]. In: eLife. 2017 ; Vol. 6.

Bibtex

@article{0319da72842641c0a7d3f34fd13d7410,
title = "Origin and evolution of transporter substrate specificity within the NPF family: [with Correction]",
abstract = "Despite vast diversity in metabolites and the matching substrate specificity of their transporters, little is known about how evolution of transporter substrate specificities is linked to emergence of substrates via evolution of biosynthetic pathways. Transporter specificity towards the recently evolved glucosinolates characteristic of Brassicales is shown to evolve prior to emergence of glucosinolate biosynthesis. Furthermore, we show that glucosinolate transporters belonging to the ubiquitous NRT1/PTR FAMILY (NPF) likely evolved from transporters of the ancestral cyanogenic glucosides found across more than 2500 species outside of the Brassicales. Biochemical characterization of orthologs along the phylogenetic lineage from cassava to A. thaliana, suggests that alterations in the electrogenicity of the transporters accompanied changes in substrate specificity. Linking the evolutionary path of transporter substrate specificities to that of the biosynthetic pathways, exemplify how transporter substrate specificities originate and evolve as new biosynthesis pathways emerge.",
author = "J{\o}rgensen, {Morten Egevang} and Deyang Xu and Christoph Crocoll and Ernst, {Heidi Asschenfeldt} and David Ram{\'i}rez and Motawie, {Mohammed Saddik} and Olsen, {Carl Erik} and Mirza, {Osman Asghar} and Nour-Eldin, {Hussam Hassan} and Halkier, {Barbara Ann}",
note = "Correction: https://doi.org/10.7554/eLife.46989",
year = "2017",
doi = "10.7554/eLife.19466",
language = "English",
volume = "6",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications Ltd.",

}

RIS

TY - JOUR

T1 - Origin and evolution of transporter substrate specificity within the NPF family

T2 - [with Correction]

AU - Jørgensen, Morten Egevang

AU - Xu, Deyang

AU - Crocoll, Christoph

AU - Ernst, Heidi Asschenfeldt

AU - Ramírez, David

AU - Motawie, Mohammed Saddik

AU - Olsen, Carl Erik

AU - Mirza, Osman Asghar

AU - Nour-Eldin, Hussam Hassan

AU - Halkier, Barbara Ann

N1 - Correction: https://doi.org/10.7554/eLife.46989

PY - 2017

Y1 - 2017

N2 - Despite vast diversity in metabolites and the matching substrate specificity of their transporters, little is known about how evolution of transporter substrate specificities is linked to emergence of substrates via evolution of biosynthetic pathways. Transporter specificity towards the recently evolved glucosinolates characteristic of Brassicales is shown to evolve prior to emergence of glucosinolate biosynthesis. Furthermore, we show that glucosinolate transporters belonging to the ubiquitous NRT1/PTR FAMILY (NPF) likely evolved from transporters of the ancestral cyanogenic glucosides found across more than 2500 species outside of the Brassicales. Biochemical characterization of orthologs along the phylogenetic lineage from cassava to A. thaliana, suggests that alterations in the electrogenicity of the transporters accompanied changes in substrate specificity. Linking the evolutionary path of transporter substrate specificities to that of the biosynthetic pathways, exemplify how transporter substrate specificities originate and evolve as new biosynthesis pathways emerge.

AB - Despite vast diversity in metabolites and the matching substrate specificity of their transporters, little is known about how evolution of transporter substrate specificities is linked to emergence of substrates via evolution of biosynthetic pathways. Transporter specificity towards the recently evolved glucosinolates characteristic of Brassicales is shown to evolve prior to emergence of glucosinolate biosynthesis. Furthermore, we show that glucosinolate transporters belonging to the ubiquitous NRT1/PTR FAMILY (NPF) likely evolved from transporters of the ancestral cyanogenic glucosides found across more than 2500 species outside of the Brassicales. Biochemical characterization of orthologs along the phylogenetic lineage from cassava to A. thaliana, suggests that alterations in the electrogenicity of the transporters accompanied changes in substrate specificity. Linking the evolutionary path of transporter substrate specificities to that of the biosynthetic pathways, exemplify how transporter substrate specificities originate and evolve as new biosynthesis pathways emerge.

UR - https://elifesciences.org/articles/46989

UR - https://doi.org/10.7554/eLife.46989

U2 - 10.7554/eLife.19466

DO - 10.7554/eLife.19466

M3 - Journal article

C2 - 28257001

VL - 6

JO - eLife

JF - eLife

SN - 2050-084X

M1 - e19466

ER -

ID: 173820422