Modification of ethylene sensitivity in ornamental plants using CRISPR/Cas9
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Modification of ethylene sensitivity in ornamental plants using CRISPR/Cas9. / Kemp, Oliver; Favero, Bruno Trevenzoli; Hegelund, Josefine Nymark; Möller, Svenning Rune; Müller, Renate; Petersen, Bent L.; Lütken, Henrik Vlk.
I International Symposium on Tropical and Subtropical Ornamentals. International Society for Horticultural Science (ISHS), 2017. p. 271-280 (Acta Horticulturae, Vol. 1167).Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review
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TY - GEN
T1 - Modification of ethylene sensitivity in ornamental plants using CRISPR/Cas9
AU - Kemp, Oliver
AU - Favero, Bruno Trevenzoli
AU - Hegelund, Josefine Nymark
AU - Möller, Svenning Rune
AU - Müller, Renate
AU - Petersen, Bent L.
AU - Lütken, Henrik Vlk
N1 - Conference code: 1
PY - 2017
Y1 - 2017
N2 - Ethylene sensitivity has for long been of interest in improving ornamental plants e.g., Kalanchöe and Campanula. We aim to investigate changes in ethylene sensitivity in economically important ornamental plants by targeting genes in the ethylene pathway using the novel precise genome editing tool; the Clustered Regularly Interspaced Palindromic Repeats (CRISPR) RNA guided Cas9 DNA nuclease (CRISPR/Cas9). CRISPR/Cas9 may be employed to introduce targeted double-stranded breaks (DSBs) at desired sites in the host genome. The DSBs will be repaired by the non-homologous end-joining (NHEJ) repair mechanism which often results in small indels and consequently gene knockout. The CRISPR/Cas9 system consists of a protein DNA nuclease (Cas9) which is guided to the target sequence by a small RNA molecule (sgRNA) that recognizes a 20 bp target sequence in the genome situated immediately downstream of a 3 bp protospacer adjacent motif (PAM). The sgRNA confers the sequence specificity of the CRISPR/Cas9 complex and may thus be designed to target virtually any sequence, a feature that has made it the method of choice within precise genetic engineering. Although most research with CRISPR/Cas9 has been conducted in prokaryote and mammalian cells, steps have been taken to implement the system in plants. Proof of function has been obtained in various plant species e.g., Arabidopsis, wheat, soybean and orange which makes it plausible that this technique could be applied to ornamental plants as well. The CRISPR/Cas9 system will be delivered using Agrobacterium tumefaciens and explant regeneration of tissue cultures to create stable transformation and mutation events.
AB - Ethylene sensitivity has for long been of interest in improving ornamental plants e.g., Kalanchöe and Campanula. We aim to investigate changes in ethylene sensitivity in economically important ornamental plants by targeting genes in the ethylene pathway using the novel precise genome editing tool; the Clustered Regularly Interspaced Palindromic Repeats (CRISPR) RNA guided Cas9 DNA nuclease (CRISPR/Cas9). CRISPR/Cas9 may be employed to introduce targeted double-stranded breaks (DSBs) at desired sites in the host genome. The DSBs will be repaired by the non-homologous end-joining (NHEJ) repair mechanism which often results in small indels and consequently gene knockout. The CRISPR/Cas9 system consists of a protein DNA nuclease (Cas9) which is guided to the target sequence by a small RNA molecule (sgRNA) that recognizes a 20 bp target sequence in the genome situated immediately downstream of a 3 bp protospacer adjacent motif (PAM). The sgRNA confers the sequence specificity of the CRISPR/Cas9 complex and may thus be designed to target virtually any sequence, a feature that has made it the method of choice within precise genetic engineering. Although most research with CRISPR/Cas9 has been conducted in prokaryote and mammalian cells, steps have been taken to implement the system in plants. Proof of function has been obtained in various plant species e.g., Arabidopsis, wheat, soybean and orange which makes it plausible that this technique could be applied to ornamental plants as well. The CRISPR/Cas9 system will be delivered using Agrobacterium tumefaciens and explant regeneration of tissue cultures to create stable transformation and mutation events.
U2 - 10.17660/ActaHortic.2017.1167.40
DO - 10.17660/ActaHortic.2017.1167.40
M3 - Article in proceedings
SN - 978-94-62611-63-4
T3 - Acta Horticulturae
SP - 271
EP - 280
BT - I International Symposium on Tropical and Subtropical Ornamentals
PB - International Society for Horticultural Science (ISHS)
T2 - International Symposium on Tropical and Subtropical Ornamentals
Y2 - 7 March 2016 through 9 March 2016
ER -
ID: 184774790