Metabolic changes and increased levels of bioactive compounds in white radish (Raphanus sativus L. Cv. 01) sprouts elicited by oligochitosan
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Metabolic changes and increased levels of bioactive compounds in white radish (Raphanus sativus L. Cv. 01) sprouts elicited by oligochitosan. / Rakpenthai, Apidet; Khaksar, Gholamreza; Burow, Meike; Olsen, Carl Erik; Sirikantaramas, Supaart.
In: Agronomy, Vol. 9, No. 8, 467, 19.08.2019, p. 1-14.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Metabolic changes and increased levels of bioactive compounds in white radish (Raphanus sativus L. Cv. 01) sprouts elicited by oligochitosan
AU - Rakpenthai, Apidet
AU - Khaksar, Gholamreza
AU - Burow, Meike
AU - Olsen, Carl Erik
AU - Sirikantaramas, Supaart
PY - 2019/8/19
Y1 - 2019/8/19
N2 - The effect of oligochitosan O-80 treatment on metabolic changes in white radish (Raphanus sativus L.) sprouts (WRS) was investigated for its potential to enhance bioactive compounds accumulation. The seeds were germinated in deionized water containing different concentrations of oligochitosan O-80 (0 (control), 5, 10, 20 and 40 ppm). Seven-day old sprouts were harvested for metabolome analysis using liquid chromatography-mass spectrometry (LC-MS) and high-performance liquid chromatography (HPLC) for phenolic compounds and glucosinolate analysis, respectively, and spectrophotometric assays to determine the total phenolic content and antioxidant capacity. Partial least squares discriminant analysis was adopted to model the data from the LC-MS and revealed that O-80 at all tested concentrations affected the metabolite profiles of the treated WRS samples. The UV chromatogram at 320 nm showed increased levels of most sinapoyl derivatives, consistent with the increased total phenolic contents. Interestingly, glucoraphasatin (4-methylthio-3-butenyl glucosinolate), a major glucosinolate detected by HPLC, was increased by 40% in the sprouts treated with 10 ppm O-80. Our results provide compelling evidence regarding the exogenous application of oligochitosan O-80 as an elicitor of bioactive metabolites in WRS.
AB - The effect of oligochitosan O-80 treatment on metabolic changes in white radish (Raphanus sativus L.) sprouts (WRS) was investigated for its potential to enhance bioactive compounds accumulation. The seeds were germinated in deionized water containing different concentrations of oligochitosan O-80 (0 (control), 5, 10, 20 and 40 ppm). Seven-day old sprouts were harvested for metabolome analysis using liquid chromatography-mass spectrometry (LC-MS) and high-performance liquid chromatography (HPLC) for phenolic compounds and glucosinolate analysis, respectively, and spectrophotometric assays to determine the total phenolic content and antioxidant capacity. Partial least squares discriminant analysis was adopted to model the data from the LC-MS and revealed that O-80 at all tested concentrations affected the metabolite profiles of the treated WRS samples. The UV chromatogram at 320 nm showed increased levels of most sinapoyl derivatives, consistent with the increased total phenolic contents. Interestingly, glucoraphasatin (4-methylthio-3-butenyl glucosinolate), a major glucosinolate detected by HPLC, was increased by 40% in the sprouts treated with 10 ppm O-80. Our results provide compelling evidence regarding the exogenous application of oligochitosan O-80 as an elicitor of bioactive metabolites in WRS.
KW - Bioactive metabolite
KW - Glucosinolate
KW - Metabolomics
KW - Oligochitosan
KW - Total phenolic content
KW - White radish sprout
U2 - 10.3390/agronomy9080467
DO - 10.3390/agronomy9080467
M3 - Journal article
AN - SCOPUS:85071115839
VL - 9
SP - 1
EP - 14
JO - Agronomy
JF - Agronomy
SN - 2073-4395
IS - 8
M1 - 467
ER -
ID: 234149038