Limited impacts of high doses of dietary copper on the gut bacterial metal resistome explain negligible co-selection of antibiotic resistance

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Limited impacts of high doses of dietary copper on the gut bacterial metal resistome explain negligible co-selection of antibiotic resistance. / Forouzandeh, Asal; Lassen, Simon Bo; Brinck, Julius Emil; Zhou, Yan Yan; Zhu, Jiaojiao; Solà-Oriol, David; Monteiro, Alessandra; Hao, Xiuli; Su, Jian Qiang; Stein, Hans H.; Pérez, J. Francisco; Brandt, Kristian K.

In: Science of the Total Environment, Vol. 889, 164183, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Forouzandeh, A, Lassen, SB, Brinck, JE, Zhou, YY, Zhu, J, Solà-Oriol, D, Monteiro, A, Hao, X, Su, JQ, Stein, HH, Pérez, JF & Brandt, KK 2023, 'Limited impacts of high doses of dietary copper on the gut bacterial metal resistome explain negligible co-selection of antibiotic resistance', Science of the Total Environment, vol. 889, 164183. https://doi.org/10.1016/j.scitotenv.2023.164183

APA

Forouzandeh, A., Lassen, S. B., Brinck, J. E., Zhou, Y. Y., Zhu, J., Solà-Oriol, D., Monteiro, A., Hao, X., Su, J. Q., Stein, H. H., Pérez, J. F., & Brandt, K. K. (2023). Limited impacts of high doses of dietary copper on the gut bacterial metal resistome explain negligible co-selection of antibiotic resistance. Science of the Total Environment, 889, [164183]. https://doi.org/10.1016/j.scitotenv.2023.164183

Vancouver

Forouzandeh A, Lassen SB, Brinck JE, Zhou YY, Zhu J, Solà-Oriol D et al. Limited impacts of high doses of dietary copper on the gut bacterial metal resistome explain negligible co-selection of antibiotic resistance. Science of the Total Environment. 2023;889. 164183. https://doi.org/10.1016/j.scitotenv.2023.164183

Author

Forouzandeh, Asal ; Lassen, Simon Bo ; Brinck, Julius Emil ; Zhou, Yan Yan ; Zhu, Jiaojiao ; Solà-Oriol, David ; Monteiro, Alessandra ; Hao, Xiuli ; Su, Jian Qiang ; Stein, Hans H. ; Pérez, J. Francisco ; Brandt, Kristian K. / Limited impacts of high doses of dietary copper on the gut bacterial metal resistome explain negligible co-selection of antibiotic resistance. In: Science of the Total Environment. 2023 ; Vol. 889.

Bibtex

@article{1554ff13020f48128298bd1862962cb4,
title = "Limited impacts of high doses of dietary copper on the gut bacterial metal resistome explain negligible co-selection of antibiotic resistance",
abstract = "High dietary intake of Cu has previously been linked to the selection of Cu resistance and co-selection of antibiotic resistance in specific gut bacteria. Based on a novel HT-qPCR metal resistance gene chip as combined with 16S rRNA gene amplicon sequencing and phenotypic resistance typing of Escherichia coli isolates, we here report the impacts of two contrasting Cu-based feed additives on the swine gut bacterial metal resistome and community assembly. DNA was extracted from fecal samples (n = 80) collected at day 26 and 116 of the experiment from 200 pigs allotted to five dietary treatments: negative control (NC) diet with 20 μg CuSO4 g−1 and four diets added 125 or 250 μg CuSO4 g−1 feed or 125 or 250 μg Cu2O g−1 feed to the NC diet. Dietary Cu supplementation reduced the relative abundance of Lactobacillus, but it had negligible impacts on bacterial community composition relative to the gut microbiome maturation effect (time). The relative importance of different bacterial community assembly processes was not markedly affected by the dietary Cu treatments, and differences in swine gut metal resistome composition could be explained primarily by differences in bacterial community composition rather than by dietary Cu treatments. High dietary Cu intake (250 μg Cu g−1) selected for phenotypic Cu resistance in E. coli isolates, but surprisingly it did not result in increased prevalence of the Cu resistance genes targeted by the HT-qPCR chip. In conclusion, the lacking impacts of dietary Cu on the gut bacterial metal resistome explain results from a previous study showing that even high therapeutic doses of dietary Cu did not cause co-selection of antibiotic resistance genes and mobile genetic elements known to harbor these genes.",
keywords = "Antibiotic resistance, Bacterial community assembly, Co-selection, Escherichia coli, Gut microbiome, Metal resistance",
author = "Asal Forouzandeh and Lassen, {Simon Bo} and Brinck, {Julius Emil} and Zhou, {Yan Yan} and Jiaojiao Zhu and David Sol{\`a}-Oriol and Alessandra Monteiro and Xiuli Hao and Su, {Jian Qiang} and Stein, {Hans H.} and P{\'e}rez, {J. Francisco} and Brandt, {Kristian K.}",
note = "Publisher Copyright: {\textcopyright} 2023 The Authors",
year = "2023",
doi = "10.1016/j.scitotenv.2023.164183",
language = "English",
volume = "889",
journal = "Science of the Total Environment",
issn = "0048-9697",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Limited impacts of high doses of dietary copper on the gut bacterial metal resistome explain negligible co-selection of antibiotic resistance

AU - Forouzandeh, Asal

AU - Lassen, Simon Bo

AU - Brinck, Julius Emil

AU - Zhou, Yan Yan

AU - Zhu, Jiaojiao

AU - Solà-Oriol, David

AU - Monteiro, Alessandra

AU - Hao, Xiuli

AU - Su, Jian Qiang

AU - Stein, Hans H.

AU - Pérez, J. Francisco

AU - Brandt, Kristian K.

N1 - Publisher Copyright: © 2023 The Authors

PY - 2023

Y1 - 2023

N2 - High dietary intake of Cu has previously been linked to the selection of Cu resistance and co-selection of antibiotic resistance in specific gut bacteria. Based on a novel HT-qPCR metal resistance gene chip as combined with 16S rRNA gene amplicon sequencing and phenotypic resistance typing of Escherichia coli isolates, we here report the impacts of two contrasting Cu-based feed additives on the swine gut bacterial metal resistome and community assembly. DNA was extracted from fecal samples (n = 80) collected at day 26 and 116 of the experiment from 200 pigs allotted to five dietary treatments: negative control (NC) diet with 20 μg CuSO4 g−1 and four diets added 125 or 250 μg CuSO4 g−1 feed or 125 or 250 μg Cu2O g−1 feed to the NC diet. Dietary Cu supplementation reduced the relative abundance of Lactobacillus, but it had negligible impacts on bacterial community composition relative to the gut microbiome maturation effect (time). The relative importance of different bacterial community assembly processes was not markedly affected by the dietary Cu treatments, and differences in swine gut metal resistome composition could be explained primarily by differences in bacterial community composition rather than by dietary Cu treatments. High dietary Cu intake (250 μg Cu g−1) selected for phenotypic Cu resistance in E. coli isolates, but surprisingly it did not result in increased prevalence of the Cu resistance genes targeted by the HT-qPCR chip. In conclusion, the lacking impacts of dietary Cu on the gut bacterial metal resistome explain results from a previous study showing that even high therapeutic doses of dietary Cu did not cause co-selection of antibiotic resistance genes and mobile genetic elements known to harbor these genes.

AB - High dietary intake of Cu has previously been linked to the selection of Cu resistance and co-selection of antibiotic resistance in specific gut bacteria. Based on a novel HT-qPCR metal resistance gene chip as combined with 16S rRNA gene amplicon sequencing and phenotypic resistance typing of Escherichia coli isolates, we here report the impacts of two contrasting Cu-based feed additives on the swine gut bacterial metal resistome and community assembly. DNA was extracted from fecal samples (n = 80) collected at day 26 and 116 of the experiment from 200 pigs allotted to five dietary treatments: negative control (NC) diet with 20 μg CuSO4 g−1 and four diets added 125 or 250 μg CuSO4 g−1 feed or 125 or 250 μg Cu2O g−1 feed to the NC diet. Dietary Cu supplementation reduced the relative abundance of Lactobacillus, but it had negligible impacts on bacterial community composition relative to the gut microbiome maturation effect (time). The relative importance of different bacterial community assembly processes was not markedly affected by the dietary Cu treatments, and differences in swine gut metal resistome composition could be explained primarily by differences in bacterial community composition rather than by dietary Cu treatments. High dietary Cu intake (250 μg Cu g−1) selected for phenotypic Cu resistance in E. coli isolates, but surprisingly it did not result in increased prevalence of the Cu resistance genes targeted by the HT-qPCR chip. In conclusion, the lacking impacts of dietary Cu on the gut bacterial metal resistome explain results from a previous study showing that even high therapeutic doses of dietary Cu did not cause co-selection of antibiotic resistance genes and mobile genetic elements known to harbor these genes.

KW - Antibiotic resistance

KW - Bacterial community assembly

KW - Co-selection

KW - Escherichia coli

KW - Gut microbiome

KW - Metal resistance

U2 - 10.1016/j.scitotenv.2023.164183

DO - 10.1016/j.scitotenv.2023.164183

M3 - Journal article

C2 - 37201857

AN - SCOPUS:85160576386

VL - 889

JO - Science of the Total Environment

JF - Science of the Total Environment

SN - 0048-9697

M1 - 164183

ER -

ID: 360826524