In vitro characterization of the Ac locus in white clover (Trifolium repens L.)
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Microsomal fractions from developing shoots of adult white clover plants (of genotype Ac Ac) and cotyledons of dark germinated clover seedlings can synthesize 2-hydroxy-2-methylpropanenitrile and 2-hydroxy-2-methylbutanenitrile, the aglycone precursors of the cyanogenic glucosides, linamarin and lotaustralin, from various precursors in the presence of NADPH. l-Valine, 2-methylpropanal oxime, and 2-methylpropanenitrile are converted to 2-hydroxy-2-methylpropanenitrile and are detected as cyanide and acetone. l-Isoleucine and 2-methylbutanal oxime are converted to 2-hydroxy-2-methylbutanenitrile and are detected as cyanide and 2-butanone. At least two steps in these conversions are missing in microsomes from plants of genotype ac ac.
Original language | English |
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Journal | Archives of Biochemistry and Biophysics |
Volume | 218 |
Issue number | 1 |
Pages (from-to) | 38-45 |
Number of pages | 8 |
ISSN | 0003-9861 |
DOIs | |
Publication status | Published - 1 Oct 1982 |
ID: 201511980