ICP-MS and LC-ICP-MS for analysis of trace element content and speciation in cereal grains

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

ICP-MS and LC-ICP-MS for analysis of trace element content and speciation in cereal grains. / Persson, D. P.; Hansen, T. H.; Laursen, K. H.; Husted, S.; Schjørring, J. K.

Plant Metabolomics: Methods and Protocols. ed. / Nigel Hardy; Robert Hall; Robert Hall. Springer, 2012. p. 193-211 (Methods in Molecular Biology, Vol. 860).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Persson, DP, Hansen, TH, Laursen, KH, Husted, S & Schjørring, JK 2012, ICP-MS and LC-ICP-MS for analysis of trace element content and speciation in cereal grains. in N Hardy, R Hall & R Hall (eds), Plant Metabolomics: Methods and Protocols. Springer, Methods in Molecular Biology, vol. 860, pp. 193-211. https://doi.org/10.1007/978-1-61779-594-7_13

APA

Persson, D. P., Hansen, T. H., Laursen, K. H., Husted, S., & Schjørring, J. K. (2012). ICP-MS and LC-ICP-MS for analysis of trace element content and speciation in cereal grains. In N. Hardy, R. Hall, & R. Hall (Eds.), Plant Metabolomics: Methods and Protocols (pp. 193-211). Springer. Methods in Molecular Biology Vol. 860 https://doi.org/10.1007/978-1-61779-594-7_13

Vancouver

Persson DP, Hansen TH, Laursen KH, Husted S, Schjørring JK. ICP-MS and LC-ICP-MS for analysis of trace element content and speciation in cereal grains. In Hardy N, Hall R, Hall R, editors, Plant Metabolomics: Methods and Protocols. Springer. 2012. p. 193-211. (Methods in Molecular Biology, Vol. 860). https://doi.org/10.1007/978-1-61779-594-7_13

Author

Persson, D. P. ; Hansen, T. H. ; Laursen, K. H. ; Husted, S. ; Schjørring, J. K. / ICP-MS and LC-ICP-MS for analysis of trace element content and speciation in cereal grains. Plant Metabolomics: Methods and Protocols. editor / Nigel Hardy ; Robert Hall ; Robert Hall. Springer, 2012. pp. 193-211 (Methods in Molecular Biology, Vol. 860).

Bibtex

@inbook{f96bce3e2c654d5084683597457180af,
title = "ICP-MS and LC-ICP-MS for analysis of trace element content and speciation in cereal grains",
abstract = "Trace elements are unevenly distributed and speciated throughout the cereal grain. The germ and the outer layers of the grain have the highest concentrations of trace elements. A large fraction of the trace elements is therefore lost during the milling process. The bioavailability of the remaining trace elements is very low. This is usually ascribed to the formation of poorly soluble complexes with the phosphorus storage compound phytic acid. Hence, analysis of the total concentration of trace elements in grain tissues must be combined with a speciation analysis in order to assess their contribution to human nutrition. This chapter deals with the fractionation of anatomically very different cereal tissues. Procedures for microscaling of digestion procedures are outlined together with requirements for the use of certified reference materials in elemental profiling of grain tissue fractions. Methods for extraction and analysis of complexes containing trace elements in the grain tissue fractions are described. Finally, the chapter concludes with criteria for choice of chromatographic methods and setting of ICP-MS instrument parameters.",
keywords = "Aleurone layer, Cereal grain, Chromatography, Endosperm, ICP-MS, Iron, Micro-nutrients, Microscaled digestion, Polyatomic interference, SEC-ICP-MS, Size exclusion, Speciation, Trace elements, Zinc",
author = "Persson, {D. P.} and Hansen, {T. H.} and Laursen, {K. H.} and S. Husted and Schj{\o}rring, {J. K.}",
year = "2012",
doi = "10.1007/978-1-61779-594-7_13",
language = "English",
isbn = "9781617795930",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "193--211",
editor = "Nigel Hardy and Robert Hall and Robert Hall",
booktitle = "Plant Metabolomics",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - ICP-MS and LC-ICP-MS for analysis of trace element content and speciation in cereal grains

AU - Persson, D. P.

AU - Hansen, T. H.

AU - Laursen, K. H.

AU - Husted, S.

AU - Schjørring, J. K.

PY - 2012

Y1 - 2012

N2 - Trace elements are unevenly distributed and speciated throughout the cereal grain. The germ and the outer layers of the grain have the highest concentrations of trace elements. A large fraction of the trace elements is therefore lost during the milling process. The bioavailability of the remaining trace elements is very low. This is usually ascribed to the formation of poorly soluble complexes with the phosphorus storage compound phytic acid. Hence, analysis of the total concentration of trace elements in grain tissues must be combined with a speciation analysis in order to assess their contribution to human nutrition. This chapter deals with the fractionation of anatomically very different cereal tissues. Procedures for microscaling of digestion procedures are outlined together with requirements for the use of certified reference materials in elemental profiling of grain tissue fractions. Methods for extraction and analysis of complexes containing trace elements in the grain tissue fractions are described. Finally, the chapter concludes with criteria for choice of chromatographic methods and setting of ICP-MS instrument parameters.

AB - Trace elements are unevenly distributed and speciated throughout the cereal grain. The germ and the outer layers of the grain have the highest concentrations of trace elements. A large fraction of the trace elements is therefore lost during the milling process. The bioavailability of the remaining trace elements is very low. This is usually ascribed to the formation of poorly soluble complexes with the phosphorus storage compound phytic acid. Hence, analysis of the total concentration of trace elements in grain tissues must be combined with a speciation analysis in order to assess their contribution to human nutrition. This chapter deals with the fractionation of anatomically very different cereal tissues. Procedures for microscaling of digestion procedures are outlined together with requirements for the use of certified reference materials in elemental profiling of grain tissue fractions. Methods for extraction and analysis of complexes containing trace elements in the grain tissue fractions are described. Finally, the chapter concludes with criteria for choice of chromatographic methods and setting of ICP-MS instrument parameters.

KW - Aleurone layer

KW - Cereal grain

KW - Chromatography

KW - Endosperm

KW - ICP-MS

KW - Iron

KW - Micro-nutrients

KW - Microscaled digestion

KW - Polyatomic interference

KW - SEC-ICP-MS

KW - Size exclusion

KW - Speciation

KW - Trace elements

KW - Zinc

U2 - 10.1007/978-1-61779-594-7_13

DO - 10.1007/978-1-61779-594-7_13

M3 - Book chapter

C2 - 22351179

AN - SCOPUS:84858113062

SN - 9781617795930

T3 - Methods in Molecular Biology

SP - 193

EP - 211

BT - Plant Metabolomics

A2 - Hardy, Nigel

A2 - Hall, Robert

A2 - Hall, Robert

PB - Springer

ER -

ID: 226545653