Histone chaperone ASF1 mediates H3.3-H4 deposition in Arabidopsis
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Histone chaperone ASF1 mediates H3.3-H4 deposition in Arabidopsis. / Zhong, Zhenhui; Wang, Yafei; Wang, Ming; Yang, Fan; Thomas, Quentin Angelo; Xue, Yan; Zhang, Yaxin; Liu, Wanlu; Jami-Alahmadi, Yasaman; Xu, Linhao; Feng, Suhua; Marquardt, Sebastian; Wohlschlegel, James A; Ausin, Israel; Jacobsen, Steven E.
In: Nature Communications, Vol. 13, 6970, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Histone chaperone ASF1 mediates H3.3-H4 deposition in Arabidopsis
AU - Zhong, Zhenhui
AU - Wang, Yafei
AU - Wang, Ming
AU - Yang, Fan
AU - Thomas, Quentin Angelo
AU - Xue, Yan
AU - Zhang, Yaxin
AU - Liu, Wanlu
AU - Jami-Alahmadi, Yasaman
AU - Xu, Linhao
AU - Feng, Suhua
AU - Marquardt, Sebastian
AU - Wohlschlegel, James A
AU - Ausin, Israel
AU - Jacobsen, Steven E
N1 - © 2022. The Author(s).
PY - 2022
Y1 - 2022
N2 - Histone chaperones and chromatin remodelers control nucleosome dynamics, which are essential for transcription, replication, and DNA repair. The histone chaperone Anti-Silencing Factor 1 (ASF1) plays a central role in facilitating CAF-1-mediated replication-dependent H3.1 deposition and HIRA-mediated replication-independent H3.3 deposition in yeast and metazoans. Whether ASF1 function is evolutionarily conserved in plants is unknown. Here, we show that Arabidopsis ASF1 proteins display a preference for the HIRA complex. Simultaneous mutation of both Arabidopsis ASF1 genes caused a decrease in chromatin density and ectopic H3.1 occupancy at loci typically enriched with H3.3. Genetic, transcriptomic, and proteomic data indicate that ASF1 proteins strongly prefers the HIRA complex over CAF-1. asf1 mutants also displayed an increase in spurious Pol II transcriptional initiation and showed defects in the maintenance of gene body CG DNA methylation and in the distribution of histone modifications. Furthermore, ectopic targeting of ASF1 caused excessive histone deposition, less accessible chromatin, and gene silencing. These findings reveal the importance of ASF1-mediated histone deposition for proper epigenetic regulation of the genome.
AB - Histone chaperones and chromatin remodelers control nucleosome dynamics, which are essential for transcription, replication, and DNA repair. The histone chaperone Anti-Silencing Factor 1 (ASF1) plays a central role in facilitating CAF-1-mediated replication-dependent H3.1 deposition and HIRA-mediated replication-independent H3.3 deposition in yeast and metazoans. Whether ASF1 function is evolutionarily conserved in plants is unknown. Here, we show that Arabidopsis ASF1 proteins display a preference for the HIRA complex. Simultaneous mutation of both Arabidopsis ASF1 genes caused a decrease in chromatin density and ectopic H3.1 occupancy at loci typically enriched with H3.3. Genetic, transcriptomic, and proteomic data indicate that ASF1 proteins strongly prefers the HIRA complex over CAF-1. asf1 mutants also displayed an increase in spurious Pol II transcriptional initiation and showed defects in the maintenance of gene body CG DNA methylation and in the distribution of histone modifications. Furthermore, ectopic targeting of ASF1 caused excessive histone deposition, less accessible chromatin, and gene silencing. These findings reveal the importance of ASF1-mediated histone deposition for proper epigenetic regulation of the genome.
KW - Arabidopsis/genetics
KW - Cell Cycle Proteins/metabolism
KW - Chromatin/genetics
KW - Chromatin Assembly Factor-1/genetics
KW - Epigenesis, Genetic
KW - Histone Chaperones/genetics
KW - Histones/genetics
KW - Molecular Chaperones/genetics
KW - Proteomics
KW - Arabidopsis Proteins
U2 - 10.1038/s41467-022-34648-0
DO - 10.1038/s41467-022-34648-0
M3 - Journal article
C2 - 36379930
VL - 13
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
M1 - 6970
ER -
ID: 327942140