High-throughput analysis of amino acids in plant materials by single quadrupole mass spectrometry
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
High-throughput analysis of amino acids in plant materials by single quadrupole mass spectrometry. / Dahl-Lassen, Rasmus; van Hecke, Jan Julien Josef; Jørgensen, Henning; Bukh, Christian; Andersen, Birgit; Schjørring, Jan Kofod.
In: Plant Methods, Vol. 14, 8, 2018.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - High-throughput analysis of amino acids in plant materials by single quadrupole mass spectrometry
AU - Dahl-Lassen, Rasmus
AU - van Hecke, Jan Julien Josef
AU - Jørgensen, Henning
AU - Bukh, Christian
AU - Andersen, Birgit
AU - Schjørring, Jan Kofod
PY - 2018
Y1 - 2018
N2 - Background: The amino acid profile of plants is an important parameter in assessments of their growth potential, resource-use efficiency and/or quality as food and feed. Screening studies may involve large number of samples but the classical amino acid analysis is limited by the fact that it is very time consuming with typical chromatographic run times of 70 min or more. Results: We have here developed a high-throughput method for analysis of amino acid profiles in plant materials. The method combines classical protein hydrolysis and derivatization with fast separation by UHPLC and detection by a single quadrupole (QDa) mass spectrometer. The chromatographic run time is reduced to 10 min and the precision, accuracy and sensitivity of the method are in line with other recent methods utilizing advanced and more expensive mass spectrometers. The sensitivity of the method is at least a factor 10 better than that of methods relying on detection by fluorescence or UV. It is possible to downscale sample size to 20 mg without compromising reproducibility, which makes the method ideal for analysis of very small sample amounts. Conclusion: The developed method allows high-throughput analysis of amino acid profiles in plant materials. The analysis is robust and accurate as well as compatible with both free amino acids and protein hydrolysates. The QDa detector offers high sensitivity and accuracy, while at the same time being relatively simple to operate and cheap to purchase, thus significantly reducing the overall analytical costs compared to methods based on more advanced mass spectrometers.
AB - Background: The amino acid profile of plants is an important parameter in assessments of their growth potential, resource-use efficiency and/or quality as food and feed. Screening studies may involve large number of samples but the classical amino acid analysis is limited by the fact that it is very time consuming with typical chromatographic run times of 70 min or more. Results: We have here developed a high-throughput method for analysis of amino acid profiles in plant materials. The method combines classical protein hydrolysis and derivatization with fast separation by UHPLC and detection by a single quadrupole (QDa) mass spectrometer. The chromatographic run time is reduced to 10 min and the precision, accuracy and sensitivity of the method are in line with other recent methods utilizing advanced and more expensive mass spectrometers. The sensitivity of the method is at least a factor 10 better than that of methods relying on detection by fluorescence or UV. It is possible to downscale sample size to 20 mg without compromising reproducibility, which makes the method ideal for analysis of very small sample amounts. Conclusion: The developed method allows high-throughput analysis of amino acid profiles in plant materials. The analysis is robust and accurate as well as compatible with both free amino acids and protein hydrolysates. The QDa detector offers high sensitivity and accuracy, while at the same time being relatively simple to operate and cheap to purchase, thus significantly reducing the overall analytical costs compared to methods based on more advanced mass spectrometers.
KW - Amino acid analysis
KW - Biomass
KW - Biorefinery
KW - Green leaves
KW - Mass spectrometry
KW - Protein extraction
U2 - 10.1186/s13007-018-0277-8
DO - 10.1186/s13007-018-0277-8
M3 - Journal article
C2 - 29375649
AN - SCOPUS:85040795931
VL - 14
JO - Plant Methods
JF - Plant Methods
SN - 1746-4811
M1 - 8
ER -
ID: 196737347