Grandmother's pesticide exposure revealed bi-generational effects in Daphnia magna
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Grandmother's pesticide exposure revealed bi-generational effects in Daphnia magna. / Poulsen, Rikke; De Fine Licht, Henrik H.; Hansen, Martin; Cedergreen, Nina.
In: Aquatic Toxicology, Vol. 236, 105861, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Grandmother's pesticide exposure revealed bi-generational effects in Daphnia magna
AU - Poulsen, Rikke
AU - De Fine Licht, Henrik H.
AU - Hansen, Martin
AU - Cedergreen, Nina
N1 - Publisher Copyright: © 2021 The Authors
PY - 2021
Y1 - 2021
N2 - Man-made chemicals are a significant contributor to the ongoing deterioration of numerous ecosystems. Currently, risk assessment of these chemicals is based on observations in a single generation of animals, despite potential adverse intergenerational effects. Here, we investigate the effect of the fungicide prochloraz across three generations of Daphnia magna. We studied both the effects of continuous exposure over all generations and the effects of first-generation (F0) exposure on two subsequent generations. Effects at different levels of biological organization from genome-wide gene expression, whole organism metabolite levels, CYP enzyme activity and key phenotypic effects, such as reproduction, were monitored. Acclimation to prochloraz was found after continuous exposure. Following F0-exposure, embryonically exposed F1-offspring showed no significant effects. However, in the potentially germline exposed F2 animals, several parameters differed significantly from controls. A direct association between these F2 effects and the toxic mode of action of prochloraz was found, showing that chemicals can be harmful not only to the directly exposed generation, but also to prenatally exposed generations and in that way effects may even appear to skip a generation. This implies that current risk assessment practices are neglecting an important aspect of toxicity, such as delayed effects across generations due to a time gap between chemical exposure and emergence of effects.
AB - Man-made chemicals are a significant contributor to the ongoing deterioration of numerous ecosystems. Currently, risk assessment of these chemicals is based on observations in a single generation of animals, despite potential adverse intergenerational effects. Here, we investigate the effect of the fungicide prochloraz across three generations of Daphnia magna. We studied both the effects of continuous exposure over all generations and the effects of first-generation (F0) exposure on two subsequent generations. Effects at different levels of biological organization from genome-wide gene expression, whole organism metabolite levels, CYP enzyme activity and key phenotypic effects, such as reproduction, were monitored. Acclimation to prochloraz was found after continuous exposure. Following F0-exposure, embryonically exposed F1-offspring showed no significant effects. However, in the potentially germline exposed F2 animals, several parameters differed significantly from controls. A direct association between these F2 effects and the toxic mode of action of prochloraz was found, showing that chemicals can be harmful not only to the directly exposed generation, but also to prenatally exposed generations and in that way effects may even appear to skip a generation. This implies that current risk assessment practices are neglecting an important aspect of toxicity, such as delayed effects across generations due to a time gap between chemical exposure and emergence of effects.
KW - Acclimation
KW - Daphnia magna
KW - Environmental toxicology
KW - Intergenerational effects
KW - Prochloraz
KW - Toxic mechanisms
U2 - 10.1016/j.aquatox.2021.105861
DO - 10.1016/j.aquatox.2021.105861
M3 - Journal article
C2 - 34049113
AN - SCOPUS:85106964683
VL - 236
JO - Aquatic Toxicology
JF - Aquatic Toxicology
SN - 0166-445X
M1 - 105861
ER -
ID: 274061313