Fungal artillery of zombie flies: Infectious spore dispersal using a soft water cannon
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Fungal artillery of zombie flies : Infectious spore dispersal using a soft water cannon. / De Ruiter, Jolet; Arnbjerg-Nielsen, Sif Fink; Herren, Pascal; Høier, Freja; De Fine Licht, Henrik H.; Jensen, Kaare H.
In: Journal of the Royal Society Interface, Vol. 16, No. 159, 20190448, 02.10.2019, p. 1-10.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Fungal artillery of zombie flies
T2 - Infectious spore dispersal using a soft water cannon
AU - De Ruiter, Jolet
AU - Arnbjerg-Nielsen, Sif Fink
AU - Herren, Pascal
AU - Høier, Freja
AU - De Fine Licht, Henrik H.
AU - Jensen, Kaare H.
PY - 2019/10/2
Y1 - 2019/10/2
N2 - Dead sporulating female fly cadavers infected by the house fly-pathogenic fungus Entomophthora muscae are attractive to healthy male flies, which by their physical inspection may mechanically trigger spore release and by their movement create whirlwind airflows that covers them in infectious conidia. The fungal artillery of E. muscae protrudes outward from the fly cadaver, and consists of a plethora of micrometric stalks that each uses a liquid-based turgor pressure build-up to eject a jet of protoplasm and the initially attached spore. The biophysical processes that regulate the release and range of spores, however, are unknown. To study the physics of ejection, we design a biomimetic ‘soft cannon’ that consists of a millimetric elastomeric barrel filled with fluid and plugged with a projectile. We precisely control the maximum pressure leading up to the ejection, and study the cannon efficiency as a function of its geometry and wall elasticity. In particular, we predict that ejection velocity decreases with spore size. The calculated flight trajectories under aerodynamic drag predict that the minimum spore size required to traverse a quiescent layer of a few millimetres around the fly cadaver is approximately 10 µm. This corroborates with the natural size of E. muscae conidia (approx. 27 µm) being large enough to traverse the boundary layer but small enough (less than 40 µm) to be lifted by air currents. Based on this understanding, we show how the fungal spores are able to reach a new host.
AB - Dead sporulating female fly cadavers infected by the house fly-pathogenic fungus Entomophthora muscae are attractive to healthy male flies, which by their physical inspection may mechanically trigger spore release and by their movement create whirlwind airflows that covers them in infectious conidia. The fungal artillery of E. muscae protrudes outward from the fly cadaver, and consists of a plethora of micrometric stalks that each uses a liquid-based turgor pressure build-up to eject a jet of protoplasm and the initially attached spore. The biophysical processes that regulate the release and range of spores, however, are unknown. To study the physics of ejection, we design a biomimetic ‘soft cannon’ that consists of a millimetric elastomeric barrel filled with fluid and plugged with a projectile. We precisely control the maximum pressure leading up to the ejection, and study the cannon efficiency as a function of its geometry and wall elasticity. In particular, we predict that ejection velocity decreases with spore size. The calculated flight trajectories under aerodynamic drag predict that the minimum spore size required to traverse a quiescent layer of a few millimetres around the fly cadaver is approximately 10 µm. This corroborates with the natural size of E. muscae conidia (approx. 27 µm) being large enough to traverse the boundary layer but small enough (less than 40 µm) to be lifted by air currents. Based on this understanding, we show how the fungal spores are able to reach a new host.
KW - Biomimetic soft cannon
KW - Dispersal range
KW - Entomophthora muscae
KW - Force-balance model
KW - Fungal spore ejection
KW - High-speed videography
U2 - 10.1098/rsif.2019.0448
DO - 10.1098/rsif.2019.0448
M3 - Journal article
C2 - 31662074
AN - SCOPUS:85074261424
VL - 16
SP - 1
EP - 10
JO - Journal of the Royal Society. Interface
JF - Journal of the Royal Society. Interface
SN - 1742-5689
IS - 159
M1 - 20190448
ER -
ID: 234148549