Functional consequences of splicing of the antisense transcript COOLAIR on FLC transcription
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Functional consequences of splicing of the antisense transcript COOLAIR on FLC transcription. / Marquardt, Sebastian; Raitskin, Oleg; Wu, Zhe; Liu, Fuquan; Sun, Qianwen; Dean, Caroline.
In: Molecular Cell, Vol. 54, No. 1, 2014, p. 156-165.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Functional consequences of splicing of the antisense transcript COOLAIR on FLC transcription
AU - Marquardt, Sebastian
AU - Raitskin, Oleg
AU - Wu, Zhe
AU - Liu, Fuquan
AU - Sun, Qianwen
AU - Dean, Caroline
N1 - Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.
PY - 2014
Y1 - 2014
N2 - Antisense transcription is widespread in many genomes; however, how much is functional is hotly debated. We are investigating functionality of a set of long noncoding antisense transcripts, collectively called COOLAIR, produced at Arabidopsis FLOWERING LOCUS C (FLC). COOLAIR initiates just downstream of the major sense transcript poly(A) site and terminates either early or extends into the FLC promoter region. We now show that splicing of COOLAIR is functionally important. This was revealed through analysis of a hypomorphic mutation in the core spliceosome component PRP8. The prp8 mutation perturbs a cotranscriptional feedback mechanism linking COOLAIR processing to FLC gene body histone demethylation and reduced FLC transcription. The importance of COOLAIR splicing in this repression mechanism was confirmed by disrupting COOLAIR production and mutating the COOLAIR proximal splice acceptor site. Our findings suggest that altered splicing of a long noncoding transcript can quantitatively modulate gene expression through cotranscriptional coupling mechanisms.
AB - Antisense transcription is widespread in many genomes; however, how much is functional is hotly debated. We are investigating functionality of a set of long noncoding antisense transcripts, collectively called COOLAIR, produced at Arabidopsis FLOWERING LOCUS C (FLC). COOLAIR initiates just downstream of the major sense transcript poly(A) site and terminates either early or extends into the FLC promoter region. We now show that splicing of COOLAIR is functionally important. This was revealed through analysis of a hypomorphic mutation in the core spliceosome component PRP8. The prp8 mutation perturbs a cotranscriptional feedback mechanism linking COOLAIR processing to FLC gene body histone demethylation and reduced FLC transcription. The importance of COOLAIR splicing in this repression mechanism was confirmed by disrupting COOLAIR production and mutating the COOLAIR proximal splice acceptor site. Our findings suggest that altered splicing of a long noncoding transcript can quantitatively modulate gene expression through cotranscriptional coupling mechanisms.
KW - Arabidopsis
KW - Arabidopsis Proteins
KW - Dealkylation
KW - Feedback, Physiological
KW - Flowers
KW - Gene Expression Regulation, Plant
KW - Histones
KW - MADS Domain Proteins
KW - Mutation
KW - RNA Splicing
KW - RNA, Antisense
KW - RNA, Long Noncoding
KW - RNA-Binding Proteins
KW - Seedlings
KW - Time Factors
KW - Transcription, Genetic
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1016/j.molcel.2014.03.026
DO - 10.1016/j.molcel.2014.03.026
M3 - Journal article
C2 - 24725596
VL - 54
SP - 156
EP - 165
JO - Molecular Cell
JF - Molecular Cell
SN - 1097-2765
IS - 1
ER -
ID: 183164621