FIONA1-mediated methylation of the 3'UTR of FLC affects FLC transcript levels and flowering in Arabidopsis

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Standard

FIONA1-mediated methylation of the 3'UTR of FLC affects FLC transcript levels and flowering in Arabidopsis. / Sun, Bin; Bhati, Kaushal Kumar; Song, Peizhe; Edwards, Ashleigh; Petri, Louise; Kruusvee, Valdeko; Blaakmeer, Anko; Dolde, Ulla; Rodrigues, Vandasue; Straub, Daniel; Yang, Junbo; Jia, Guifang; Wenkel, Stephan.

In: PLOS Genetics, Vol. 18, No. 9, e1010386, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Sun, B, Bhati, KK, Song, P, Edwards, A, Petri, L, Kruusvee, V, Blaakmeer, A, Dolde, U, Rodrigues, V, Straub, D, Yang, J, Jia, G & Wenkel, S 2022, 'FIONA1-mediated methylation of the 3'UTR of FLC affects FLC transcript levels and flowering in Arabidopsis', PLOS Genetics, vol. 18, no. 9, e1010386. https://doi.org/10.1371/journal.pgen.1010386

APA

Sun, B., Bhati, K. K., Song, P., Edwards, A., Petri, L., Kruusvee, V., Blaakmeer, A., Dolde, U., Rodrigues, V., Straub, D., Yang, J., Jia, G., & Wenkel, S. (2022). FIONA1-mediated methylation of the 3'UTR of FLC affects FLC transcript levels and flowering in Arabidopsis. PLOS Genetics, 18(9), [e1010386]. https://doi.org/10.1371/journal.pgen.1010386

Vancouver

Sun B, Bhati KK, Song P, Edwards A, Petri L, Kruusvee V et al. FIONA1-mediated methylation of the 3'UTR of FLC affects FLC transcript levels and flowering in Arabidopsis. PLOS Genetics. 2022;18(9). e1010386. https://doi.org/10.1371/journal.pgen.1010386

Author

Sun, Bin ; Bhati, Kaushal Kumar ; Song, Peizhe ; Edwards, Ashleigh ; Petri, Louise ; Kruusvee, Valdeko ; Blaakmeer, Anko ; Dolde, Ulla ; Rodrigues, Vandasue ; Straub, Daniel ; Yang, Junbo ; Jia, Guifang ; Wenkel, Stephan. / FIONA1-mediated methylation of the 3'UTR of FLC affects FLC transcript levels and flowering in Arabidopsis. In: PLOS Genetics. 2022 ; Vol. 18, No. 9.

Bibtex

@article{4ad2da63229c47db843713f93da31d35,
title = "FIONA1-mediated methylation of the 3'UTR of FLC affects FLC transcript levels and flowering in Arabidopsis",
abstract = "Adenosine bases of RNA can be transiently modified by the deposition of a methyl-group to form N6-methyladenosine (m6A). This adenosine-methylation is an ancient process and the enzymes involved are evolutionary highly conserved. A genetic screen designed to identify suppressors of late flowering transgenic Arabidopsis plants overexpressing the miP1a microProtein yielded a new allele of the FIONA1 (FIO1) m6A-methyltransferase. To characterize the early flowering phenotype of fio1 mutant plants we employed an integrative approach of mRNA-seq, Nanopore direct RNA-sequencing and meRIP-seq to identify differentially expressed transcripts as well as differentially methylated RNAs. We provide evidence that FIO1 is the elusive methyltransferase responsible for the 3'-end methylation of the FLOWERING LOCUS C (FLC) transcript. Furthermore, our genetic and biochemical data suggest that 3′-methylation stabilizes FLC mRNAs and non-methylated FLC is a target for rapid degradatio. ",
author = "Bin Sun and Bhati, {Kaushal Kumar} and Peizhe Song and Ashleigh Edwards and Louise Petri and Valdeko Kruusvee and Anko Blaakmeer and Ulla Dolde and Vandasue Rodrigues and Daniel Straub and Junbo Yang and Guifang Jia and Stephan Wenkel",
note = "Publisher Copyright: Copyright {\textcopyright} 2022 Sun et al.",
year = "2022",
doi = "10.1371/journal.pgen.1010386",
language = "English",
volume = "18",
journal = "P L o S Genetics",
issn = "1553-7390",
publisher = "Public Library of Science",
number = "9",

}

RIS

TY - JOUR

T1 - FIONA1-mediated methylation of the 3'UTR of FLC affects FLC transcript levels and flowering in Arabidopsis

AU - Sun, Bin

AU - Bhati, Kaushal Kumar

AU - Song, Peizhe

AU - Edwards, Ashleigh

AU - Petri, Louise

AU - Kruusvee, Valdeko

AU - Blaakmeer, Anko

AU - Dolde, Ulla

AU - Rodrigues, Vandasue

AU - Straub, Daniel

AU - Yang, Junbo

AU - Jia, Guifang

AU - Wenkel, Stephan

N1 - Publisher Copyright: Copyright © 2022 Sun et al.

PY - 2022

Y1 - 2022

N2 - Adenosine bases of RNA can be transiently modified by the deposition of a methyl-group to form N6-methyladenosine (m6A). This adenosine-methylation is an ancient process and the enzymes involved are evolutionary highly conserved. A genetic screen designed to identify suppressors of late flowering transgenic Arabidopsis plants overexpressing the miP1a microProtein yielded a new allele of the FIONA1 (FIO1) m6A-methyltransferase. To characterize the early flowering phenotype of fio1 mutant plants we employed an integrative approach of mRNA-seq, Nanopore direct RNA-sequencing and meRIP-seq to identify differentially expressed transcripts as well as differentially methylated RNAs. We provide evidence that FIO1 is the elusive methyltransferase responsible for the 3'-end methylation of the FLOWERING LOCUS C (FLC) transcript. Furthermore, our genetic and biochemical data suggest that 3′-methylation stabilizes FLC mRNAs and non-methylated FLC is a target for rapid degradatio.

AB - Adenosine bases of RNA can be transiently modified by the deposition of a methyl-group to form N6-methyladenosine (m6A). This adenosine-methylation is an ancient process and the enzymes involved are evolutionary highly conserved. A genetic screen designed to identify suppressors of late flowering transgenic Arabidopsis plants overexpressing the miP1a microProtein yielded a new allele of the FIONA1 (FIO1) m6A-methyltransferase. To characterize the early flowering phenotype of fio1 mutant plants we employed an integrative approach of mRNA-seq, Nanopore direct RNA-sequencing and meRIP-seq to identify differentially expressed transcripts as well as differentially methylated RNAs. We provide evidence that FIO1 is the elusive methyltransferase responsible for the 3'-end methylation of the FLOWERING LOCUS C (FLC) transcript. Furthermore, our genetic and biochemical data suggest that 3′-methylation stabilizes FLC mRNAs and non-methylated FLC is a target for rapid degradatio.

U2 - 10.1371/journal.pgen.1010386

DO - 10.1371/journal.pgen.1010386

M3 - Journal article

C2 - 36166469

AN - SCOPUS:85139572029

VL - 18

JO - P L o S Genetics

JF - P L o S Genetics

SN - 1553-7390

IS - 9

M1 - e1010386

ER -

ID: 337987571