Evidence for conifer sucrose transporters’ functioning in the light-dependent adjustment of sugar allocation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Evidence for conifer sucrose transporters’ functioning in the light-dependent adjustment of sugar allocation. / Han, Xiaoyu; Gao, Chen; Liang, Buyou; Cui, Jingxuan; Xu, Qiyu; Schulz, Alexander; Liesche, Johannes.

In: Tree Physiology, Vol. 42, No. 3, 2022, p. 488-500.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Han, X, Gao, C, Liang, B, Cui, J, Xu, Q, Schulz, A & Liesche, J 2022, 'Evidence for conifer sucrose transporters’ functioning in the light-dependent adjustment of sugar allocation', Tree Physiology, vol. 42, no. 3, pp. 488-500. https://doi.org/10.1093/treephys/tpab149

APA

Han, X., Gao, C., Liang, B., Cui, J., Xu, Q., Schulz, A., & Liesche, J. (2022). Evidence for conifer sucrose transporters’ functioning in the light-dependent adjustment of sugar allocation. Tree Physiology, 42(3), 488-500. https://doi.org/10.1093/treephys/tpab149

Vancouver

Han X, Gao C, Liang B, Cui J, Xu Q, Schulz A et al. Evidence for conifer sucrose transporters’ functioning in the light-dependent adjustment of sugar allocation. Tree Physiology. 2022;42(3):488-500. https://doi.org/10.1093/treephys/tpab149

Author

Han, Xiaoyu ; Gao, Chen ; Liang, Buyou ; Cui, Jingxuan ; Xu, Qiyu ; Schulz, Alexander ; Liesche, Johannes. / Evidence for conifer sucrose transporters’ functioning in the light-dependent adjustment of sugar allocation. In: Tree Physiology. 2022 ; Vol. 42, No. 3. pp. 488-500.

Bibtex

@article{f01c78b2d1bd412e8b6e5c35f40b456c,
title = "Evidence for conifer sucrose transporters{\textquoteright} functioning in the light-dependent adjustment of sugar allocation",
abstract = "Sucrose is the central unit of carbon and energy in plants. Active intercellular transport of sucrose is mediated by sucrose transporters (SUTs), genes for which have been found in the genomes of all land plants. However, they have only been assigned functions in angiosperm species. Here, we cloned two types of SUTs from two gymnosperms, the conifers Cedrus deodara (Roxb. G. Don) and Pinus massoniana Lambert, and analyzed their sucrose transport activities. Uptake of the fluorescent sucrose-analog esculin into tobacco epidermis cells expressing the conifer SUT confirmed their transport ability. To determine their function in planta, we investigated their mRNA abundance in relation to photosynthesis and sugar levels in leaves, inner bark, wood and roots. Combined with measurements of protein abundance and immunolocalization of C. deodara SUTs, our results suggest a role for CdSUT1G and CdSUT2 in supporting phloem transport under varying environmental conditions. The implications of these findings regarding conifer physiology and SUT evolution are discussed.",
author = "Xiaoyu Han and Chen Gao and Buyou Liang and Jingxuan Cui and Qiyu Xu and Alexander Schulz and Johannes Liesche",
year = "2022",
doi = "10.1093/treephys/tpab149",
language = "English",
volume = "42",
pages = "488--500",
journal = "Tree Physiology",
issn = "0829-318X",
publisher = "Oxford University Press",
number = "3",

}

RIS

TY - JOUR

T1 - Evidence for conifer sucrose transporters’ functioning in the light-dependent adjustment of sugar allocation

AU - Han, Xiaoyu

AU - Gao, Chen

AU - Liang, Buyou

AU - Cui, Jingxuan

AU - Xu, Qiyu

AU - Schulz, Alexander

AU - Liesche, Johannes

PY - 2022

Y1 - 2022

N2 - Sucrose is the central unit of carbon and energy in plants. Active intercellular transport of sucrose is mediated by sucrose transporters (SUTs), genes for which have been found in the genomes of all land plants. However, they have only been assigned functions in angiosperm species. Here, we cloned two types of SUTs from two gymnosperms, the conifers Cedrus deodara (Roxb. G. Don) and Pinus massoniana Lambert, and analyzed their sucrose transport activities. Uptake of the fluorescent sucrose-analog esculin into tobacco epidermis cells expressing the conifer SUT confirmed their transport ability. To determine their function in planta, we investigated their mRNA abundance in relation to photosynthesis and sugar levels in leaves, inner bark, wood and roots. Combined with measurements of protein abundance and immunolocalization of C. deodara SUTs, our results suggest a role for CdSUT1G and CdSUT2 in supporting phloem transport under varying environmental conditions. The implications of these findings regarding conifer physiology and SUT evolution are discussed.

AB - Sucrose is the central unit of carbon and energy in plants. Active intercellular transport of sucrose is mediated by sucrose transporters (SUTs), genes for which have been found in the genomes of all land plants. However, they have only been assigned functions in angiosperm species. Here, we cloned two types of SUTs from two gymnosperms, the conifers Cedrus deodara (Roxb. G. Don) and Pinus massoniana Lambert, and analyzed their sucrose transport activities. Uptake of the fluorescent sucrose-analog esculin into tobacco epidermis cells expressing the conifer SUT confirmed their transport ability. To determine their function in planta, we investigated their mRNA abundance in relation to photosynthesis and sugar levels in leaves, inner bark, wood and roots. Combined with measurements of protein abundance and immunolocalization of C. deodara SUTs, our results suggest a role for CdSUT1G and CdSUT2 in supporting phloem transport under varying environmental conditions. The implications of these findings regarding conifer physiology and SUT evolution are discussed.

U2 - 10.1093/treephys/tpab149

DO - 10.1093/treephys/tpab149

M3 - Journal article

C2 - 35020944

VL - 42

SP - 488

EP - 500

JO - Tree Physiology

JF - Tree Physiology

SN - 0829-318X

IS - 3

ER -

ID: 289910258