Differential influence of Bacillus subtilis strains on Arabidopsis root architecture through common and distinct plant hormonal pathways
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Differential influence of Bacillus subtilis strains on Arabidopsis root architecture through common and distinct plant hormonal pathways. / Jensen, Camilla Niketa Gadomska; Pang, Janet Ka Yan; Hahn, Charlotte Marie; Gottardi, Michele; Husted, Søren; Moelbak, Lars; Kovács, Ákos T.; Fimognari, Lorenzo; Schulz, Alexander.
In: Plant Science, Vol. 339, 111936, 2024.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Differential influence of Bacillus subtilis strains on Arabidopsis root architecture through common and distinct plant hormonal pathways
AU - Jensen, Camilla Niketa Gadomska
AU - Pang, Janet Ka Yan
AU - Hahn, Charlotte Marie
AU - Gottardi, Michele
AU - Husted, Søren
AU - Moelbak, Lars
AU - Kovács, Ákos T.
AU - Fimognari, Lorenzo
AU - Schulz, Alexander
N1 - Publisher Copyright: © 2023 The Authors
PY - 2024
Y1 - 2024
N2 - Plant growth-promoting microbes (PGPM) can enhance crop yield and health, but knowledge of their mode-of-action is limited. We studied the influence of two Bacillus subtilis strains, the natural isolate ALC_02 and the domesticated 168 Gö, on Arabidopsis and hypothesized that they modify the root architecture by modulating hormone transport or signaling. Both bacteria promoted increase of shoot and root surface area in vitro, but through different root anatomical traits. Mutant plants deficient in auxin transport or signaling responded less to the bacterial strains than the wild-type, and application of the auxin transport inhibitor NPA strongly reduced the influence of the strains. Both bacteria produced auxin and enhanced shoot auxin levels in DR5::GUS reporter plants. Accordingly, most of the beneficial effects of the strains were dependent on functional auxin transport and signaling, while only 168 Gö depended on functional ethylene signaling. As expected, only ALC_02 stimulated plant growth in soil, unlike 168 Gö that was previously reported to have reduced biofilms. Collectively, the results highlight that B. subtilis strains can have strikingly different plant growth-promoting properties, dependent on what experimental setup they are tested in, and the importance of choosing the right PGPM for a desired root phenotype.
AB - Plant growth-promoting microbes (PGPM) can enhance crop yield and health, but knowledge of their mode-of-action is limited. We studied the influence of two Bacillus subtilis strains, the natural isolate ALC_02 and the domesticated 168 Gö, on Arabidopsis and hypothesized that they modify the root architecture by modulating hormone transport or signaling. Both bacteria promoted increase of shoot and root surface area in vitro, but through different root anatomical traits. Mutant plants deficient in auxin transport or signaling responded less to the bacterial strains than the wild-type, and application of the auxin transport inhibitor NPA strongly reduced the influence of the strains. Both bacteria produced auxin and enhanced shoot auxin levels in DR5::GUS reporter plants. Accordingly, most of the beneficial effects of the strains were dependent on functional auxin transport and signaling, while only 168 Gö depended on functional ethylene signaling. As expected, only ALC_02 stimulated plant growth in soil, unlike 168 Gö that was previously reported to have reduced biofilms. Collectively, the results highlight that B. subtilis strains can have strikingly different plant growth-promoting properties, dependent on what experimental setup they are tested in, and the importance of choosing the right PGPM for a desired root phenotype.
KW - Auxin
KW - Beneficial bacteria
KW - Microbe-associated molecular pattern
KW - Plant-growth promoting microbes
KW - Root architecture
KW - Root hairs
U2 - 10.1016/j.plantsci.2023.111936
DO - 10.1016/j.plantsci.2023.111936
M3 - Journal article
C2 - 38042415
AN - SCOPUS:85179618252
VL - 339
JO - Plant Science
JF - Plant Science
SN - 0168-9452
M1 - 111936
ER -
ID: 380151646