Design and direct assembly of synthesized uracil-containing non-clonal DNA fragments into vectors by USERTM cloning
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Design and direct assembly of synthesized uracil-containing non-clonal DNA fragments into vectors by USERTM cloning. / Jørgensen, Morten Egevang; Wulff, Nikolai; Nafisi, Majse; Xu, Deyang; Wang, Cuiwei; Lambertz, Sophie Konstanze; Belew, Zeinu Mussa; Nour-Eldin, Hussam Hassan.
In: Bio-protocol, Vol. 7, No. 22, e2615, 2017.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Design and direct assembly of synthesized uracil-containing non-clonal DNA fragments into vectors by USERTM cloning
AU - Jørgensen, Morten Egevang
AU - Wulff, Nikolai
AU - Nafisi, Majse
AU - Xu, Deyang
AU - Wang, Cuiwei
AU - Lambertz, Sophie Konstanze
AU - Belew, Zeinu Mussa
AU - Nour-Eldin, Hussam Hassan
PY - 2017
Y1 - 2017
N2 - This protocol describes how to order and directly assemble uracil-containing non-clonal DNAfragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal ofmaking synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol ishighly efficient and would be compatible with uracil-containing non-clonal DNA fragments obtained fromany synthesizing company. The protocol drastically reduces time and handling between receiving thesynthesized DNA fragments and transforming with vector and DNA fragment(s).
AB - This protocol describes how to order and directly assemble uracil-containing non-clonal DNAfragments by uracil excision based cloning (USER cloning). The protocol was generated with the goal ofmaking synthesized non-clonal DNA fragments directly compatible with USERTM cloning. The protocol ishighly efficient and would be compatible with uracil-containing non-clonal DNA fragments obtained fromany synthesizing company. The protocol drastically reduces time and handling between receiving thesynthesized DNA fragments and transforming with vector and DNA fragment(s).
U2 - 10.21769/BioProtoc.2615
DO - 10.21769/BioProtoc.2615
M3 - Journal article
C2 - 34595288
VL - 7
JO - Bio-protocol
JF - Bio-protocol
SN - 2331-8325
IS - 22
M1 - e2615
ER -
ID: 188708667