Cullin 4-ring finger-ligase plays a key role in the control of endoreplication cycles in Arabidopsis trichomes

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Cullin 4-ring finger-ligase plays a key role in the control of endoreplication cycles in Arabidopsis trichomes. / Roodbarkelari, Farshad; Bramsiepe, Jonathan; Weinl, Christina; Marquardt, Sebastian; Novák, Béla; Jakoby, Marc J; Lechner, Esther; Genschik, Pascal; Schnittger, Arp.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 34, 2010, p. 15275-15280.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Roodbarkelari, F, Bramsiepe, J, Weinl, C, Marquardt, S, Novák, B, Jakoby, MJ, Lechner, E, Genschik, P & Schnittger, A 2010, 'Cullin 4-ring finger-ligase plays a key role in the control of endoreplication cycles in Arabidopsis trichomes', Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 34, pp. 15275-15280. https://doi.org/10.1073/pnas.1006941107

APA

Roodbarkelari, F., Bramsiepe, J., Weinl, C., Marquardt, S., Novák, B., Jakoby, M. J., Lechner, E., Genschik, P., & Schnittger, A. (2010). Cullin 4-ring finger-ligase plays a key role in the control of endoreplication cycles in Arabidopsis trichomes. Proceedings of the National Academy of Sciences of the United States of America, 107(34), 15275-15280. https://doi.org/10.1073/pnas.1006941107

Vancouver

Roodbarkelari F, Bramsiepe J, Weinl C, Marquardt S, Novák B, Jakoby MJ et al. Cullin 4-ring finger-ligase plays a key role in the control of endoreplication cycles in Arabidopsis trichomes. Proceedings of the National Academy of Sciences of the United States of America. 2010;107(34):15275-15280. https://doi.org/10.1073/pnas.1006941107

Author

Roodbarkelari, Farshad ; Bramsiepe, Jonathan ; Weinl, Christina ; Marquardt, Sebastian ; Novák, Béla ; Jakoby, Marc J ; Lechner, Esther ; Genschik, Pascal ; Schnittger, Arp. / Cullin 4-ring finger-ligase plays a key role in the control of endoreplication cycles in Arabidopsis trichomes. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 34. pp. 15275-15280.

Bibtex

@article{1fb715a402ae49a4bd5ffcc59cad3c75,
title = "Cullin 4-ring finger-ligase plays a key role in the control of endoreplication cycles in Arabidopsis trichomes",
abstract = "One of the predominant cell-cycle programs found in mature tissues is endoreplication, also known as endoreduplication, that leads to cellular polyploidy. A key question for the understanding of endoreplication cycles is how oscillating levels of cyclin-dependent kinase activity are generated that control repeated rounds of DNA replication. The APC/C performs a pivotal function in the mitotic cell cycle by promoting anaphase and paving the road for a new round of DNA replication. However, using marker lines and plants in which APC/C components are knocked down, we show here that outgrowing and endoreplicating Arabidopsis leaf hairs display no or very little APC/C activity. Instead we find that RBX1-containing Cullin-RING E3 ubiquitin-Ligases (CRLs) are of central importance for the progression through endoreplication cycles; in particular, we have identified CULLIN4 as a major regulator of endoreplication in Arabidopsis trichomes. We have incorporated our findings into a bio-mathematical simulation presenting a robust two-step model of endoreplication control with one type of cyclin-dependent kinase inhibitor function for entry and a CRL-dependent oscillation of cyclin-dependent kinase activity via degradation of a second type of CDK inhibitor during endoreplication cycles.",
keywords = "Arabidopsis, Arabidopsis Proteins, Carrier Proteins, Cell Cycle, Cullin Proteins, DNA Replication, DNA, Plant, Genes, Plant, Microscopy, Electron, Scanning, Models, Biological, Mutation, Plant Structures, Plants, Genetically Modified, Polyploidy, RNA Interference, Journal Article, Research Support, Non-U.S. Gov't",
author = "Farshad Roodbarkelari and Jonathan Bramsiepe and Christina Weinl and Sebastian Marquardt and B{\'e}la Nov{\'a}k and Jakoby, {Marc J} and Esther Lechner and Pascal Genschik and Arp Schnittger",
year = "2010",
doi = "10.1073/pnas.1006941107",
language = "English",
volume = "107",
pages = "15275--15280",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "34",

}

RIS

TY - JOUR

T1 - Cullin 4-ring finger-ligase plays a key role in the control of endoreplication cycles in Arabidopsis trichomes

AU - Roodbarkelari, Farshad

AU - Bramsiepe, Jonathan

AU - Weinl, Christina

AU - Marquardt, Sebastian

AU - Novák, Béla

AU - Jakoby, Marc J

AU - Lechner, Esther

AU - Genschik, Pascal

AU - Schnittger, Arp

PY - 2010

Y1 - 2010

N2 - One of the predominant cell-cycle programs found in mature tissues is endoreplication, also known as endoreduplication, that leads to cellular polyploidy. A key question for the understanding of endoreplication cycles is how oscillating levels of cyclin-dependent kinase activity are generated that control repeated rounds of DNA replication. The APC/C performs a pivotal function in the mitotic cell cycle by promoting anaphase and paving the road for a new round of DNA replication. However, using marker lines and plants in which APC/C components are knocked down, we show here that outgrowing and endoreplicating Arabidopsis leaf hairs display no or very little APC/C activity. Instead we find that RBX1-containing Cullin-RING E3 ubiquitin-Ligases (CRLs) are of central importance for the progression through endoreplication cycles; in particular, we have identified CULLIN4 as a major regulator of endoreplication in Arabidopsis trichomes. We have incorporated our findings into a bio-mathematical simulation presenting a robust two-step model of endoreplication control with one type of cyclin-dependent kinase inhibitor function for entry and a CRL-dependent oscillation of cyclin-dependent kinase activity via degradation of a second type of CDK inhibitor during endoreplication cycles.

AB - One of the predominant cell-cycle programs found in mature tissues is endoreplication, also known as endoreduplication, that leads to cellular polyploidy. A key question for the understanding of endoreplication cycles is how oscillating levels of cyclin-dependent kinase activity are generated that control repeated rounds of DNA replication. The APC/C performs a pivotal function in the mitotic cell cycle by promoting anaphase and paving the road for a new round of DNA replication. However, using marker lines and plants in which APC/C components are knocked down, we show here that outgrowing and endoreplicating Arabidopsis leaf hairs display no or very little APC/C activity. Instead we find that RBX1-containing Cullin-RING E3 ubiquitin-Ligases (CRLs) are of central importance for the progression through endoreplication cycles; in particular, we have identified CULLIN4 as a major regulator of endoreplication in Arabidopsis trichomes. We have incorporated our findings into a bio-mathematical simulation presenting a robust two-step model of endoreplication control with one type of cyclin-dependent kinase inhibitor function for entry and a CRL-dependent oscillation of cyclin-dependent kinase activity via degradation of a second type of CDK inhibitor during endoreplication cycles.

KW - Arabidopsis

KW - Arabidopsis Proteins

KW - Carrier Proteins

KW - Cell Cycle

KW - Cullin Proteins

KW - DNA Replication

KW - DNA, Plant

KW - Genes, Plant

KW - Microscopy, Electron, Scanning

KW - Models, Biological

KW - Mutation

KW - Plant Structures

KW - Plants, Genetically Modified

KW - Polyploidy

KW - RNA Interference

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1073/pnas.1006941107

DO - 10.1073/pnas.1006941107

M3 - Journal article

C2 - 20696906

VL - 107

SP - 15275

EP - 15280

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 34

ER -

ID: 183164910