Cellulose amendment promotes P solubilization by Penicillium aculeatum in non-sterilized soil
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Cellulose amendment promotes P solubilization by Penicillium aculeatum in non-sterilized soil. / Gómez-Muñoz, Beatriz; Efthymiou, Aikaterini; Dubey, Mukesh; Sølve, Jonathan; Nicolaisen, Mette; Jensen, Dan F.; Nybroe, Ole; Larsen, John.
In: Fungal Biology, Vol. 126, No. 5, 2022, p. 356-365.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Cellulose amendment promotes P solubilization by Penicillium aculeatum in non-sterilized soil
AU - Gómez-Muñoz, Beatriz
AU - Efthymiou, Aikaterini
AU - Dubey, Mukesh
AU - Sølve, Jonathan
AU - Nicolaisen, Mette
AU - Jensen, Dan F.
AU - Nybroe, Ole
AU - Larsen, John
N1 - Publisher Copyright: © 2022
PY - 2022
Y1 - 2022
N2 - Successful application of microbial biofertilizers, such as phosphorus (P) solubilizing fungi to agroecosystems, is constrained from the lack of knowledge about their ecology; for example in terms of how they respond to an external input of carbon (C) to get established in the soil. In two soil incubation experiments we examined the performance of the P solubilizing fungus Penicillium aculeatum in non-sterile and semi-sterile (γ-irradiated) soil with different C and P sources. Results from the first experiment with C sources showed that starch and cellulose generally improved P solubilization by P. aculeatum measured as water extractable P (Pwep), though only significantly in non-sterile soil. This coincided with an increased population density of P. aculeatum measured with a hygromycin B resistant strain of this fungus. Soil respiration used to measure soil microbial activity was overall much higher in treatments with C compounds than without C in both non-sterile and semi-sterile soil. However, soil respiration was highest with cellulose in semi-sterile soil, especially in combination with P. aculeatum. Hence, for the second experiment with P sources (tricalcium phosphate (TCP) and sewage sludge ash) cellulose was used as a C source for P. aculeatum growth in all treatments. Main results showed that P. aculeatum in combination with cellulose soil amendment increased soil Pwep independent of soil sterilization and P source treatments. Soil resin P (Pres) and microbial P (Pmic), which represents stocks of potentially plant available P, were also affected from P. aculeatum inoculation. Increased soil Pres from TCP and sewage sludge ash was observed with P. aculeatum independent of soil type. On the other hand soil Pmic was higher after P. aculeatum inoculation only in semi-sterile soil. Population density of P. aculeatum measured with qPCR was maintained or increased in non-sterile and semi-sterile soil, respectively, compared to the original inoculum load of P. aculeatum. In conclusion, our results underline the importance of C source addition for P. aculeatum if used as a biofertilizer. For this, cellulose seems to be a promising option promoting P. aculeatum growth and P solubilization also in non-sterilized soil.
AB - Successful application of microbial biofertilizers, such as phosphorus (P) solubilizing fungi to agroecosystems, is constrained from the lack of knowledge about their ecology; for example in terms of how they respond to an external input of carbon (C) to get established in the soil. In two soil incubation experiments we examined the performance of the P solubilizing fungus Penicillium aculeatum in non-sterile and semi-sterile (γ-irradiated) soil with different C and P sources. Results from the first experiment with C sources showed that starch and cellulose generally improved P solubilization by P. aculeatum measured as water extractable P (Pwep), though only significantly in non-sterile soil. This coincided with an increased population density of P. aculeatum measured with a hygromycin B resistant strain of this fungus. Soil respiration used to measure soil microbial activity was overall much higher in treatments with C compounds than without C in both non-sterile and semi-sterile soil. However, soil respiration was highest with cellulose in semi-sterile soil, especially in combination with P. aculeatum. Hence, for the second experiment with P sources (tricalcium phosphate (TCP) and sewage sludge ash) cellulose was used as a C source for P. aculeatum growth in all treatments. Main results showed that P. aculeatum in combination with cellulose soil amendment increased soil Pwep independent of soil sterilization and P source treatments. Soil resin P (Pres) and microbial P (Pmic), which represents stocks of potentially plant available P, were also affected from P. aculeatum inoculation. Increased soil Pres from TCP and sewage sludge ash was observed with P. aculeatum independent of soil type. On the other hand soil Pmic was higher after P. aculeatum inoculation only in semi-sterile soil. Population density of P. aculeatum measured with qPCR was maintained or increased in non-sterile and semi-sterile soil, respectively, compared to the original inoculum load of P. aculeatum. In conclusion, our results underline the importance of C source addition for P. aculeatum if used as a biofertilizer. For this, cellulose seems to be a promising option promoting P. aculeatum growth and P solubilization also in non-sterilized soil.
KW - Microbial P
KW - Phosphorous solubilizing microorganisms
KW - Resin P
KW - Sewage sludge ash
KW - Water extractable P
U2 - 10.1016/j.funbio.2022.03.003
DO - 10.1016/j.funbio.2022.03.003
M3 - Journal article
C2 - 35501031
AN - SCOPUS:85127341091
VL - 126
SP - 356
EP - 365
JO - Fungal Biology
JF - Fungal Biology
SN - 1878-6146
IS - 5
ER -
ID: 310144405