Biodegradation kinetics testing of two hydrophobic UVCBs - potential for substrate toxicity supports testing at low concentrations

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Biodegradation kinetics testing of two hydrophobic UVCBs - potential for substrate toxicity supports testing at low concentrations. / Hammershoj, Rikke; Sjøholm, Karina K.; Birch, Heidi; Brandt, Kristian K.; Mayer, Philipp.

In: Environmental Science Processes & Impacts, Vol. 22, No. 11, 2020, p. 2172-2180.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hammershoj, R, Sjøholm, KK, Birch, H, Brandt, KK & Mayer, P 2020, 'Biodegradation kinetics testing of two hydrophobic UVCBs - potential for substrate toxicity supports testing at low concentrations', Environmental Science Processes & Impacts, vol. 22, no. 11, pp. 2172-2180. https://doi.org/10.1039/d0em00288g

APA

Hammershoj, R., Sjøholm, K. K., Birch, H., Brandt, K. K., & Mayer, P. (2020). Biodegradation kinetics testing of two hydrophobic UVCBs - potential for substrate toxicity supports testing at low concentrations. Environmental Science Processes & Impacts, 22(11), 2172-2180. https://doi.org/10.1039/d0em00288g

Vancouver

Hammershoj R, Sjøholm KK, Birch H, Brandt KK, Mayer P. Biodegradation kinetics testing of two hydrophobic UVCBs - potential for substrate toxicity supports testing at low concentrations. Environmental Science Processes & Impacts. 2020;22(11):2172-2180. https://doi.org/10.1039/d0em00288g

Author

Hammershoj, Rikke ; Sjøholm, Karina K. ; Birch, Heidi ; Brandt, Kristian K. ; Mayer, Philipp. / Biodegradation kinetics testing of two hydrophobic UVCBs - potential for substrate toxicity supports testing at low concentrations. In: Environmental Science Processes & Impacts. 2020 ; Vol. 22, No. 11. pp. 2172-2180.

Bibtex

@article{fa86d1a219c84e70b717d67df9d8e725,
title = "Biodegradation kinetics testing of two hydrophobic UVCBs - potential for substrate toxicity supports testing at low concentrations",
abstract = "The biodegradation kinetics of UVCB substances (unknown or variable composition, complex reaction products or biological materials) should be determined below the solubility limit to avoid experimental artefacts by the non-dissolved mixture. Recently, we reported delayed biodegradation kinetics of single petroleum hydrocarbons even at concentrations just below the solubility limit and attributed this to toxicity. The present study aimed to determine the concentration effect on biodegradation kinetics for constituents in two UVCBs, using surface water from a rural stream as the inoculum. Parallel biodegradation tests of diesel and lavender oil were conducted at concentrations just below the solubility limit and two orders of magnitude lower. The biodegradation kinetics of diesel oil constituents were generally similar at the two concentrations, which coincided with the stimulation of bacterial productivity (growth) at both concentrations, determined by [H-3]leucine incorporation. By contrast, the biodegradation of lavender oil constituents was significantly delayed or even halted at the high test concentration. This was consistent with lavender oil stimulating bacterial growth at low concentration but inhibiting it at high concentration. The delayed biodegradation kinetics of lavender oil constituents at high concentration was best explained by mixture toxicity near the solubility limit. Consequently, biodegradation testing of hydrophobic UVCBs should be conducted at low, environmentally relevant concentrations ensuring that mixture toxicity does not affect the biodegradation kinetics.",
keywords = "PRIMARY AEROBIC BIODEGRADATION, ORGANIC-CHEMICALS, PROTEIN-SYNTHESIS, CRUDE-OIL, GASOLINE, HYDROCARBONS, ANTIBIOTICS, THYMIDINE, LEUCINE, BALANCE",
author = "Rikke Hammershoj and Sj{\o}holm, {Karina K.} and Heidi Birch and Brandt, {Kristian K.} and Philipp Mayer",
year = "2020",
doi = "10.1039/d0em00288g",
language = "English",
volume = "22",
pages = "2172--2180",
journal = "Journal of Environmental Monitoring",
issn = "1464-0325",
publisher = "Royal Society of Chemistry",
number = "11",

}

RIS

TY - JOUR

T1 - Biodegradation kinetics testing of two hydrophobic UVCBs - potential for substrate toxicity supports testing at low concentrations

AU - Hammershoj, Rikke

AU - Sjøholm, Karina K.

AU - Birch, Heidi

AU - Brandt, Kristian K.

AU - Mayer, Philipp

PY - 2020

Y1 - 2020

N2 - The biodegradation kinetics of UVCB substances (unknown or variable composition, complex reaction products or biological materials) should be determined below the solubility limit to avoid experimental artefacts by the non-dissolved mixture. Recently, we reported delayed biodegradation kinetics of single petroleum hydrocarbons even at concentrations just below the solubility limit and attributed this to toxicity. The present study aimed to determine the concentration effect on biodegradation kinetics for constituents in two UVCBs, using surface water from a rural stream as the inoculum. Parallel biodegradation tests of diesel and lavender oil were conducted at concentrations just below the solubility limit and two orders of magnitude lower. The biodegradation kinetics of diesel oil constituents were generally similar at the two concentrations, which coincided with the stimulation of bacterial productivity (growth) at both concentrations, determined by [H-3]leucine incorporation. By contrast, the biodegradation of lavender oil constituents was significantly delayed or even halted at the high test concentration. This was consistent with lavender oil stimulating bacterial growth at low concentration but inhibiting it at high concentration. The delayed biodegradation kinetics of lavender oil constituents at high concentration was best explained by mixture toxicity near the solubility limit. Consequently, biodegradation testing of hydrophobic UVCBs should be conducted at low, environmentally relevant concentrations ensuring that mixture toxicity does not affect the biodegradation kinetics.

AB - The biodegradation kinetics of UVCB substances (unknown or variable composition, complex reaction products or biological materials) should be determined below the solubility limit to avoid experimental artefacts by the non-dissolved mixture. Recently, we reported delayed biodegradation kinetics of single petroleum hydrocarbons even at concentrations just below the solubility limit and attributed this to toxicity. The present study aimed to determine the concentration effect on biodegradation kinetics for constituents in two UVCBs, using surface water from a rural stream as the inoculum. Parallel biodegradation tests of diesel and lavender oil were conducted at concentrations just below the solubility limit and two orders of magnitude lower. The biodegradation kinetics of diesel oil constituents were generally similar at the two concentrations, which coincided with the stimulation of bacterial productivity (growth) at both concentrations, determined by [H-3]leucine incorporation. By contrast, the biodegradation of lavender oil constituents was significantly delayed or even halted at the high test concentration. This was consistent with lavender oil stimulating bacterial growth at low concentration but inhibiting it at high concentration. The delayed biodegradation kinetics of lavender oil constituents at high concentration was best explained by mixture toxicity near the solubility limit. Consequently, biodegradation testing of hydrophobic UVCBs should be conducted at low, environmentally relevant concentrations ensuring that mixture toxicity does not affect the biodegradation kinetics.

KW - PRIMARY AEROBIC BIODEGRADATION

KW - ORGANIC-CHEMICALS

KW - PROTEIN-SYNTHESIS

KW - CRUDE-OIL

KW - GASOLINE

KW - HYDROCARBONS

KW - ANTIBIOTICS

KW - THYMIDINE

KW - LEUCINE

KW - BALANCE

U2 - 10.1039/d0em00288g

DO - 10.1039/d0em00288g

M3 - Journal article

C2 - 33000828

VL - 22

SP - 2172

EP - 2180

JO - Journal of Environmental Monitoring

JF - Journal of Environmental Monitoring

SN - 1464-0325

IS - 11

ER -

ID: 252770319