Assay of flippase activity in proteoliposomes using fluorescent lipid derivatives

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Assay of flippase activity in proteoliposomes using fluorescent lipid derivatives. / Marek, Magdalena; Günther-Pomorski, Thomas.

P-Type ATPases: methods and protocols. ed. / Maike Bublitz. Vol. 2. Springer, 2016. p. 181-191 (Methods in Molecular Biology, Vol. 1377).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Marek, M & Günther-Pomorski, T 2016, Assay of flippase activity in proteoliposomes using fluorescent lipid derivatives. in M Bublitz (ed.), P-Type ATPases: methods and protocols. vol. 2., Springer, Methods in Molecular Biology, vol. 1377, pp. 181-191. https://doi.org/10.1007/978-1-4939-3179-8_18

APA

Marek, M., & Günther-Pomorski, T. (2016). Assay of flippase activity in proteoliposomes using fluorescent lipid derivatives. In M. Bublitz (Ed.), P-Type ATPases: methods and protocols (Vol. 2., pp. 181-191). Springer. Methods in Molecular Biology Vol. 1377 https://doi.org/10.1007/978-1-4939-3179-8_18

Vancouver

Marek M, Günther-Pomorski T. Assay of flippase activity in proteoliposomes using fluorescent lipid derivatives. In Bublitz M, editor, P-Type ATPases: methods and protocols. Vol. 2. Springer. 2016. p. 181-191. (Methods in Molecular Biology, Vol. 1377). https://doi.org/10.1007/978-1-4939-3179-8_18

Author

Marek, Magdalena ; Günther-Pomorski, Thomas. / Assay of flippase activity in proteoliposomes using fluorescent lipid derivatives. P-Type ATPases: methods and protocols. editor / Maike Bublitz. Vol. 2. Springer, 2016. pp. 181-191 (Methods in Molecular Biology, Vol. 1377).

Bibtex

@inbook{0ad588e067fe443292ba0a63ca95ec70,
title = "Assay of flippase activity in proteoliposomes using fluorescent lipid derivatives",
abstract = "Specific membrane proteins, termed lipid flippases, play a central role in facilitating the movement of lipids across cellular membranes. In this protocol, we describe the reconstitution of ATP-driven lipid flippases in liposomes and the analysis of their in vitro flippase activity based on the use of fluorescent lipid derivatives. Working with purified and reconstituted systems provides a well-defined experimental setup and allows to directly characterize these membrane proteins at the molecular level.",
keywords = "ATP-Binding Cassette Transporters, Adenosine Triphosphate, Biological Transport, Cell Membrane, Dithionite, Fluorescence, Lipids, Membrane Proteins, Molecular Biology, Proteolipids, Journal Article, Research Support, Non-U.S. Gov't",
author = "Magdalena Marek and Thomas G{\"u}nther-Pomorski",
year = "2016",
doi = "10.1007/978-1-4939-3179-8_18",
language = "English",
isbn = "978-1-4939-3178-1",
volume = "2.",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "181--191",
editor = "Maike Bublitz",
booktitle = "P-Type ATPases",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - Assay of flippase activity in proteoliposomes using fluorescent lipid derivatives

AU - Marek, Magdalena

AU - Günther-Pomorski, Thomas

PY - 2016

Y1 - 2016

N2 - Specific membrane proteins, termed lipid flippases, play a central role in facilitating the movement of lipids across cellular membranes. In this protocol, we describe the reconstitution of ATP-driven lipid flippases in liposomes and the analysis of their in vitro flippase activity based on the use of fluorescent lipid derivatives. Working with purified and reconstituted systems provides a well-defined experimental setup and allows to directly characterize these membrane proteins at the molecular level.

AB - Specific membrane proteins, termed lipid flippases, play a central role in facilitating the movement of lipids across cellular membranes. In this protocol, we describe the reconstitution of ATP-driven lipid flippases in liposomes and the analysis of their in vitro flippase activity based on the use of fluorescent lipid derivatives. Working with purified and reconstituted systems provides a well-defined experimental setup and allows to directly characterize these membrane proteins at the molecular level.

KW - ATP-Binding Cassette Transporters

KW - Adenosine Triphosphate

KW - Biological Transport

KW - Cell Membrane

KW - Dithionite

KW - Fluorescence

KW - Lipids

KW - Membrane Proteins

KW - Molecular Biology

KW - Proteolipids

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1007/978-1-4939-3179-8_18

DO - 10.1007/978-1-4939-3179-8_18

M3 - Book chapter

C2 - 26695033

SN - 978-1-4939-3178-1

VL - 2.

T3 - Methods in Molecular Biology

SP - 181

EP - 191

BT - P-Type ATPases

A2 - Bublitz, Maike

PB - Springer

ER -

ID: 169109211