An H+-ATPase assay: Proton pumping and ATPase activity determined simultaneously in the same sample
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
An H+-ATPase assay : Proton pumping and ATPase activity determined simultaneously in the same sample. / Palmgren, Michael Gjedde.
In: Plant Physiology, Vol. 94, No. 3, 1990, p. 882-886.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - An H+-ATPase assay
T2 - Proton pumping and ATPase activity determined simultaneously in the same sample
AU - Palmgren, Michael Gjedde
PY - 1990
Y1 - 1990
N2 - A continuous spectrophotometric assay of H+-ATPase activity was developed by combining two well-known methods for measuring proton pumping and ATPase activity. Proton uptake into plasma membrane vesicles from Avena sativa L. (cv Rhiannon) was monitored as the absorbance decrease at 495 nm of the ΔpH probe acridine orange. Simultaneously, ATPase activity was measured by following the absorbance decrease at 340 nanometers by coupling ATP hydrolysis enzymatically to the oxidation of NADH. This H+-ATPase assay is convenient for determining the relative relationship between ATP hydrolysis and proton pumping.
AB - A continuous spectrophotometric assay of H+-ATPase activity was developed by combining two well-known methods for measuring proton pumping and ATPase activity. Proton uptake into plasma membrane vesicles from Avena sativa L. (cv Rhiannon) was monitored as the absorbance decrease at 495 nm of the ΔpH probe acridine orange. Simultaneously, ATPase activity was measured by following the absorbance decrease at 340 nanometers by coupling ATP hydrolysis enzymatically to the oxidation of NADH. This H+-ATPase assay is convenient for determining the relative relationship between ATP hydrolysis and proton pumping.
UR - http://www.scopus.com/inward/record.url?scp=0000001959&partnerID=8YFLogxK
U2 - 10.1104/pp.94.3.882
DO - 10.1104/pp.94.3.882
M3 - Journal article
AN - SCOPUS:0000001959
VL - 94
SP - 882
EP - 886
JO - Plant Physiology
JF - Plant Physiology
SN - 0032-0889
IS - 3
ER -
ID: 245001679