AEF1/MPR25 is implicated in RNA editing of plastid atpF and mitochondrial nad5, and also promotes atpF splicing in Arabidopsis and rice
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AEF1/MPR25 is implicated in RNA editing of plastid atpF and mitochondrial nad5, and also promotes atpF splicing in Arabidopsis and rice. / Yap, Aaron; Kindgren, Peter Robert; Colas des Francs-Small, Catherine; Kazama, Tomohiko; Tanz, Sandra K; Toriyama, Kinya; Small, Ian.
In: Plant Journal, Vol. 81, No. 5, 2015, p. 661-9.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - AEF1/MPR25 is implicated in RNA editing of plastid atpF and mitochondrial nad5, and also promotes atpF splicing in Arabidopsis and rice
AU - Yap, Aaron
AU - Kindgren, Peter Robert
AU - Colas des Francs-Small, Catherine
AU - Kazama, Tomohiko
AU - Tanz, Sandra K
AU - Toriyama, Kinya
AU - Small, Ian
N1 - © 2015 The Authors The Plant Journal © 2015 John Wiley & Sons Ltd.
PY - 2015
Y1 - 2015
N2 - RNA editing is an essential mechanism that modifies target cytidines to uridine in both mitochondrial and plastid mRNA. Target sites are recognized by pentatricopeptide repeat (PPR) proteins. Using bioinformatics predictions based on the code describing sequence recognition by PPR proteins, we have identified an Arabidopsis editing factor required for editing of atpF in plastids. A loss-of-function mutation in ATPF EDITING FACTOR 1 (AEF1, AT3G22150) results in severe variegation, presumably due to decreased plastid ATP synthase levels. Loss of editing at the atpF site is coupled with a large decrease in splicing of the atpF transcript, even though the editing site is within an exon and 53 nucleotides distant from the splice site. The rice orthologue of AEF1, MPR25, has been reported to be required for editing of a site in mitochondrial nad5 transcripts, and we confirm that editing of the same site is affected in the Arabidopsis aef1 mutant. We also show that splicing of chloroplast atpF transcripts is affected in the rice mpr25 mutant. AEF1 is thus highly unusual for an RNA editing specificity factor in that it has functions in both organelles.
AB - RNA editing is an essential mechanism that modifies target cytidines to uridine in both mitochondrial and plastid mRNA. Target sites are recognized by pentatricopeptide repeat (PPR) proteins. Using bioinformatics predictions based on the code describing sequence recognition by PPR proteins, we have identified an Arabidopsis editing factor required for editing of atpF in plastids. A loss-of-function mutation in ATPF EDITING FACTOR 1 (AEF1, AT3G22150) results in severe variegation, presumably due to decreased plastid ATP synthase levels. Loss of editing at the atpF site is coupled with a large decrease in splicing of the atpF transcript, even though the editing site is within an exon and 53 nucleotides distant from the splice site. The rice orthologue of AEF1, MPR25, has been reported to be required for editing of a site in mitochondrial nad5 transcripts, and we confirm that editing of the same site is affected in the Arabidopsis aef1 mutant. We also show that splicing of chloroplast atpF transcripts is affected in the rice mpr25 mutant. AEF1 is thus highly unusual for an RNA editing specificity factor in that it has functions in both organelles.
KW - Arabidopsis
KW - Arabidopsis Proteins
KW - Chloroplast Proteins
KW - DNA-Binding Proteins
KW - Mitochondrial Proteins
KW - Mutation
KW - Oryza
KW - Plant Proteins
KW - Plastids
KW - RNA Editing
KW - RNA Splicing
KW - RNA, Chloroplast
KW - RNA, Messenger
KW - Transcription Factors
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
U2 - 10.1111/tpj.12756
DO - 10.1111/tpj.12756
M3 - Journal article
C2 - 25585673
VL - 81
SP - 661
EP - 669
JO - Plant Journal
JF - Plant Journal
SN - 0960-7412
IS - 5
ER -
ID: 183163710