Activity of root-knot nematodes associated with composition of a nematode-attached microbiome and the surrounding soil microbiota
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Activity of root-knot nematodes associated with composition of a nematode-attached microbiome and the surrounding soil microbiota. / Topalović, Olivera; Bak, Frederik; Santos, Susana; Sikder, Md Maniruzzaman; Sapkota, Rumakanta; Ekelund, Flemming; Nicolaisen, Mette Haubjerg; Vestergård, Mette.
In: FEMS Microbiology Ecology, Vol. 99, No. 9, fiad091, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Activity of root-knot nematodes associated with composition of a nematode-attached microbiome and the surrounding soil microbiota
AU - Topalović, Olivera
AU - Bak, Frederik
AU - Santos, Susana
AU - Sikder, Md Maniruzzaman
AU - Sapkota, Rumakanta
AU - Ekelund, Flemming
AU - Nicolaisen, Mette Haubjerg
AU - Vestergård, Mette
N1 - Publisher Copyright: © 2023 The Author(s).
PY - 2023
Y1 - 2023
N2 - We investigated if activity of the pre-infective juveniles (J2s) of root-knot nematodes is linked to the recruitment of a specific microbiome on the nematode surface and/or to the composition of the surrounding microbiota. For this, we determined the J2 activity (active vs. non-motile, which referred to dead and immobile J2s) upon a 3-day incubation in soil suspensions and studied the composition of bacteria, protists, and fungi present on the nematode surface and in the suspensions using amplicon sequencing of the 16S/18S rRNA genes, and ITS region. We also amended suspensions with Pseudomonas protegens strain CHA0 to study its effects on J2 activity and microbial composition. The J2 activity was suppressed in soil suspensions, but increased when suspensions were amended with P. protegens CHA0. The active and non-motile J2s differed in the composition of surface-attached bacteria, which was altered by the presence of P. protegens CHA0 in the soil suspensions. The bacterial genera Algoriphagus, Pedobacter, and Bdellovibrio were enriched on active J2s and may have protected the J2s against antagonists. The incubation time appeared short for attachment of fungi and protists. Altogether, our study is a step forward in disentangling the complex nematode-microbe interactions in soil for more successful nematode control.
AB - We investigated if activity of the pre-infective juveniles (J2s) of root-knot nematodes is linked to the recruitment of a specific microbiome on the nematode surface and/or to the composition of the surrounding microbiota. For this, we determined the J2 activity (active vs. non-motile, which referred to dead and immobile J2s) upon a 3-day incubation in soil suspensions and studied the composition of bacteria, protists, and fungi present on the nematode surface and in the suspensions using amplicon sequencing of the 16S/18S rRNA genes, and ITS region. We also amended suspensions with Pseudomonas protegens strain CHA0 to study its effects on J2 activity and microbial composition. The J2 activity was suppressed in soil suspensions, but increased when suspensions were amended with P. protegens CHA0. The active and non-motile J2s differed in the composition of surface-attached bacteria, which was altered by the presence of P. protegens CHA0 in the soil suspensions. The bacterial genera Algoriphagus, Pedobacter, and Bdellovibrio were enriched on active J2s and may have protected the J2s against antagonists. The incubation time appeared short for attachment of fungi and protists. Altogether, our study is a step forward in disentangling the complex nematode-microbe interactions in soil for more successful nematode control.
KW - 16S/18S rRNA genes
KW - ITS
KW - Meloidogyne spp
KW - nematode protective microbiome
KW - nematode suppressive microbiome
KW - Pseudomonas protegens CHA0
U2 - 10.1093/femsec/fiad091
DO - 10.1093/femsec/fiad091
M3 - Journal article
C2 - 37553158
AN - SCOPUS:85168797223
VL - 99
JO - F E M S Microbiology Ecology
JF - F E M S Microbiology Ecology
SN - 0168-6496
IS - 9
M1 - fiad091
ER -
ID: 374642528