A 2-kb Mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A 2-kb Mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement. / Zhang, Hongxiang; Xie, Jiatao; Fu, Yanping; Cheng, Jiasen; Qu, Zheng; Zhao, Zhenzhen; Cheng, Shufen; Chen, Tao; Li, Bo; Wang, Qianqian; Liu, Xinqiang; Tian, Binnian; Collinge, David B.; Jiang, Daohong.

In: Molecular Plant, Vol. 13, No. 10, 2020, p. 1420-1433.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zhang, H, Xie, J, Fu, Y, Cheng, J, Qu, Z, Zhao, Z, Cheng, S, Chen, T, Li, B, Wang, Q, Liu, X, Tian, B, Collinge, DB & Jiang, D 2020, 'A 2-kb Mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement', Molecular Plant, vol. 13, no. 10, pp. 1420-1433. https://doi.org/10.1016/j.molp.2020.08.016

APA

Zhang, H., Xie, J., Fu, Y., Cheng, J., Qu, Z., Zhao, Z., Cheng, S., Chen, T., Li, B., Wang, Q., Liu, X., Tian, B., Collinge, D. B., & Jiang, D. (2020). A 2-kb Mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement. Molecular Plant, 13(10), 1420-1433. https://doi.org/10.1016/j.molp.2020.08.016

Vancouver

Zhang H, Xie J, Fu Y, Cheng J, Qu Z, Zhao Z et al. A 2-kb Mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement. Molecular Plant. 2020;13(10):1420-1433. https://doi.org/10.1016/j.molp.2020.08.016

Author

Zhang, Hongxiang ; Xie, Jiatao ; Fu, Yanping ; Cheng, Jiasen ; Qu, Zheng ; Zhao, Zhenzhen ; Cheng, Shufen ; Chen, Tao ; Li, Bo ; Wang, Qianqian ; Liu, Xinqiang ; Tian, Binnian ; Collinge, David B. ; Jiang, Daohong. / A 2-kb Mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement. In: Molecular Plant. 2020 ; Vol. 13, No. 10. pp. 1420-1433.

Bibtex

@article{56047fb3f2174046b30b38abd18e47e8,
title = "A 2-kb Mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement",
abstract = "Mycoviruses are viruses that infect fungi, and hypovirulence-associated mycoviruses have the potential to control fungal diseases. However, it is unclear how mycovirus-mediated hypovirulent strains live and survive in the field, and no mycovirus has been applied for field crop protection. In this study, we found that a previously identified small DNA mycovirus (SsHADV-1) can convert its host, Sclerotinia sclerotiorum, from a typical necrotrophic pathogen to a beneficial endophytic fungus. SsHADV-1 downregulates the expression of key pathogenicity factor genes in S. sclerotiorum during infection. When growing in rapeseed, the SsHADV-1-infected strain DT-8 significantly regulates the expression of rapeseed genes involved in defense, hormone signaling, and circadian rhythm pathways. As a result, plant growth is promoted and disease resistance is enhanced. Field experiments showed that spraying DT-8 at the early flowering stage can reduce the disease severity of rapeseed stem rot by 67.6% and improve yield by 14.9%. Moreover, we discovered that SsHADV-1 could also infect other S. sclerotiorum strains on DT-8-inoculated plants and that DT-8 could be recovered from dead plants. These findings suggest that the mycoviruses may have the ability to shape the origin of endophytism. Our discoveries suggest that mycoviruses may influence the origin of endophytism and may also offer a novel strategy for disease control in which mycovirus-infected strains are used to improve crop health and release mycoviruses into the field.",
keywords = "mycovirus, Sclerotinia sclerotiorum, endophyte, rapeseed stem rot, biological control, Brassica napus, CHESTNUT BLIGHT FUNGUS, BIOLOGICAL-CONTROL, SCLEROTINIA-SCLEROTIORUM, DNA MYCOVIRUS, PLANT, GENE, HYPOVIRULENCE, RESISTANCE, GROWTH, VIRUS",
author = "Hongxiang Zhang and Jiatao Xie and Yanping Fu and Jiasen Cheng and Zheng Qu and Zhenzhen Zhao and Shufen Cheng and Tao Chen and Bo Li and Qianqian Wang and Xinqiang Liu and Binnian Tian and Collinge, {David B.} and Daohong Jiang",
year = "2020",
doi = "10.1016/j.molp.2020.08.016",
language = "English",
volume = "13",
pages = "1420--1433",
journal = "Molecular Plant",
issn = "1674-2052",
publisher = "Cell Press",
number = "10",

}

RIS

TY - JOUR

T1 - A 2-kb Mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement

AU - Zhang, Hongxiang

AU - Xie, Jiatao

AU - Fu, Yanping

AU - Cheng, Jiasen

AU - Qu, Zheng

AU - Zhao, Zhenzhen

AU - Cheng, Shufen

AU - Chen, Tao

AU - Li, Bo

AU - Wang, Qianqian

AU - Liu, Xinqiang

AU - Tian, Binnian

AU - Collinge, David B.

AU - Jiang, Daohong

PY - 2020

Y1 - 2020

N2 - Mycoviruses are viruses that infect fungi, and hypovirulence-associated mycoviruses have the potential to control fungal diseases. However, it is unclear how mycovirus-mediated hypovirulent strains live and survive in the field, and no mycovirus has been applied for field crop protection. In this study, we found that a previously identified small DNA mycovirus (SsHADV-1) can convert its host, Sclerotinia sclerotiorum, from a typical necrotrophic pathogen to a beneficial endophytic fungus. SsHADV-1 downregulates the expression of key pathogenicity factor genes in S. sclerotiorum during infection. When growing in rapeseed, the SsHADV-1-infected strain DT-8 significantly regulates the expression of rapeseed genes involved in defense, hormone signaling, and circadian rhythm pathways. As a result, plant growth is promoted and disease resistance is enhanced. Field experiments showed that spraying DT-8 at the early flowering stage can reduce the disease severity of rapeseed stem rot by 67.6% and improve yield by 14.9%. Moreover, we discovered that SsHADV-1 could also infect other S. sclerotiorum strains on DT-8-inoculated plants and that DT-8 could be recovered from dead plants. These findings suggest that the mycoviruses may have the ability to shape the origin of endophytism. Our discoveries suggest that mycoviruses may influence the origin of endophytism and may also offer a novel strategy for disease control in which mycovirus-infected strains are used to improve crop health and release mycoviruses into the field.

AB - Mycoviruses are viruses that infect fungi, and hypovirulence-associated mycoviruses have the potential to control fungal diseases. However, it is unclear how mycovirus-mediated hypovirulent strains live and survive in the field, and no mycovirus has been applied for field crop protection. In this study, we found that a previously identified small DNA mycovirus (SsHADV-1) can convert its host, Sclerotinia sclerotiorum, from a typical necrotrophic pathogen to a beneficial endophytic fungus. SsHADV-1 downregulates the expression of key pathogenicity factor genes in S. sclerotiorum during infection. When growing in rapeseed, the SsHADV-1-infected strain DT-8 significantly regulates the expression of rapeseed genes involved in defense, hormone signaling, and circadian rhythm pathways. As a result, plant growth is promoted and disease resistance is enhanced. Field experiments showed that spraying DT-8 at the early flowering stage can reduce the disease severity of rapeseed stem rot by 67.6% and improve yield by 14.9%. Moreover, we discovered that SsHADV-1 could also infect other S. sclerotiorum strains on DT-8-inoculated plants and that DT-8 could be recovered from dead plants. These findings suggest that the mycoviruses may have the ability to shape the origin of endophytism. Our discoveries suggest that mycoviruses may influence the origin of endophytism and may also offer a novel strategy for disease control in which mycovirus-infected strains are used to improve crop health and release mycoviruses into the field.

KW - mycovirus

KW - Sclerotinia sclerotiorum

KW - endophyte

KW - rapeseed stem rot

KW - biological control

KW - Brassica napus

KW - CHESTNUT BLIGHT FUNGUS

KW - BIOLOGICAL-CONTROL

KW - SCLEROTINIA-SCLEROTIORUM

KW - DNA MYCOVIRUS

KW - PLANT

KW - GENE

KW - HYPOVIRULENCE

KW - RESISTANCE

KW - GROWTH

KW - VIRUS

U2 - 10.1016/j.molp.2020.08.016

DO - 10.1016/j.molp.2020.08.016

M3 - Journal article

C2 - 32998002

VL - 13

SP - 1420

EP - 1433

JO - Molecular Plant

JF - Molecular Plant

SN - 1674-2052

IS - 10

ER -

ID: 250866244