Response of microbial taxonomic and nitrogen functional attributes to elevated nitrate in suburban groundwater
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Response of microbial taxonomic and nitrogen functional attributes to elevated nitrate in suburban groundwater. / Zhang, Ling-Zhi; He, Wei; Huang, Fu-Yi; He, Wei; Zhou, Pengpeng; Chen, Cuibai; Rensing, Christopher; Brandt, Kristian Koefoed; He, Jiangtao; Liu, Fei; Zhao, Yi; Guo, Huaming.
In: Science of the Total Environment, Vol. 874, 162524, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Response of microbial taxonomic and nitrogen functional attributes to elevated nitrate in suburban groundwater
AU - Zhang, Ling-Zhi
AU - He, Wei
AU - Huang, Fu-Yi
AU - He, Wei
AU - Zhou, Pengpeng
AU - Chen, Cuibai
AU - Rensing, Christopher
AU - Brandt, Kristian Koefoed
AU - He, Jiangtao
AU - Liu, Fei
AU - Zhao, Yi
AU - Guo, Huaming
N1 - Publisher Copyright: © 2023 Elsevier B.V.
PY - 2023
Y1 - 2023
N2 - Anthropogenic nitrogen (N) input has led to elevated levels of nitrate nitrogen (NO3−-N) in the groundwater. However, insights into the responses of the microbial community and its N metabolic functionality to elevated NO3−-N in suburban groundwater are still limited. Here, we explored the microbial taxonomy, N metabolic attributes, and their responses to NO3−-N pollution in groundwaters from Chaobai River catchment (CR) and Huai River catchment (HR) in Beijing, China. Results showed that average NO3−-N and NH4+-N concentrations in CR groundwater were 1.7 and 3.0 folds of those in HR. NO3−-N was the dominant nitrogen specie both in HR and CR groundwater (over 80 %). Significantly different structures and compositions of the microbial communities and N cycling gene profiles were found between CR groundwater and HR groundwater (p < 0.05), with CR groundwater harboring significantly lower microbial richness and abundance of N metabolic genes. However, denitrification was the dominant microbial N cycling process in both CR and HR groundwater. Strong associations among NO3−-N, NH4+-N, microbial taxonomic, and N functional attributes were found (p < 0.05), suggesting denitrifiers and Candidatus_Brocadia might serve as potential featured biomarkers for the elevated NO3−-N and NH4+-N concentration in groundwater. Path analysis further revealed the significant effect of NO3−-N on the overall microbial N functionality and microbial denitrification (p < 0.05). Collectively, our results provide field evidence that elevated levels of NO3−-N and NH4+-N under different hydrogeologic conditions had a significant effect on the microbial taxonomic and N functional attributes in groundwater, with potential implications for improving sustainable N management and risk assessment of groundwater.
AB - Anthropogenic nitrogen (N) input has led to elevated levels of nitrate nitrogen (NO3−-N) in the groundwater. However, insights into the responses of the microbial community and its N metabolic functionality to elevated NO3−-N in suburban groundwater are still limited. Here, we explored the microbial taxonomy, N metabolic attributes, and their responses to NO3−-N pollution in groundwaters from Chaobai River catchment (CR) and Huai River catchment (HR) in Beijing, China. Results showed that average NO3−-N and NH4+-N concentrations in CR groundwater were 1.7 and 3.0 folds of those in HR. NO3−-N was the dominant nitrogen specie both in HR and CR groundwater (over 80 %). Significantly different structures and compositions of the microbial communities and N cycling gene profiles were found between CR groundwater and HR groundwater (p < 0.05), with CR groundwater harboring significantly lower microbial richness and abundance of N metabolic genes. However, denitrification was the dominant microbial N cycling process in both CR and HR groundwater. Strong associations among NO3−-N, NH4+-N, microbial taxonomic, and N functional attributes were found (p < 0.05), suggesting denitrifiers and Candidatus_Brocadia might serve as potential featured biomarkers for the elevated NO3−-N and NH4+-N concentration in groundwater. Path analysis further revealed the significant effect of NO3−-N on the overall microbial N functionality and microbial denitrification (p < 0.05). Collectively, our results provide field evidence that elevated levels of NO3−-N and NH4+-N under different hydrogeologic conditions had a significant effect on the microbial taxonomic and N functional attributes in groundwater, with potential implications for improving sustainable N management and risk assessment of groundwater.
KW - Ammonium
KW - Denitrification
KW - Groundwater
KW - Microbial nitrogen cycling
KW - Nitrate
U2 - 10.1016/j.scitotenv.2023.162524
DO - 10.1016/j.scitotenv.2023.162524
M3 - Journal article
C2 - 36868285
AN - SCOPUS:85149938080
VL - 874
JO - Science of the Total Environment
JF - Science of the Total Environment
SN - 0048-9697
M1 - 162524
ER -
ID: 342678960