Increasing flexibility in two-dimensional liquid chromatography by pulsed elution of the first dimension: a proof of concept
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Increasing flexibility in two-dimensional liquid chromatography by pulsed elution of the first dimension : a proof of concept. / Jakobsen, Simon S.; Christensen, Jan H.; Verdier, Sylvain; Mallet, Claude R.; Nielsen, Nikoline Juul.
In: Analytical Chemistry, Vol. 89, No. 17, 2017, p. 8723-8730.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Increasing flexibility in two-dimensional liquid chromatography by pulsed elution of the first dimension
T2 - a proof of concept
AU - Jakobsen, Simon S.
AU - Christensen, Jan H.
AU - Verdier, Sylvain
AU - Mallet, Claude R.
AU - Nielsen, Nikoline Juul
PY - 2017
Y1 - 2017
N2 - This work demonstrates the development of an online two-dimensional liquid chromatography (2D-LC) method where the first dimension column is eluted by a sequence of pulses of increasing eluotropic strength generated by the LC pumps (pulsed-elution 2D-LC). Between the pulses, the first dimension is kept in a no-elution state using low eluent strength. The eluate from the first dimension is actively modulated using trap columns and subsequently analyzed in the second dimension. We demonstrate that by tuning the length and eluotropic strength of the pulses, peaks with retention factors in water, kw, above 150 can be manipulated to elute in 3-4 pulses. The no-elution state can be kept for 1-10 min with only minor changes as to which and how many pulses the peaks elute in. Pulsed-elution 2D-LC combined with active modulation tackles three of the main challenges encountered in 2D-LC and specifically online comprehensive 2D-LC: undersampling, difficulties in refocusing, and lack of flexibility in the selection of column dimensions and flow rates because the two dimensions constrain each other. The pulsed-elution 2D-LC was applied for the analysis of a basic fraction of vacuum gas oil. Peak capacity was 4018 for a 540 min analysis and 4610 for a 1040 min analysis.
AB - This work demonstrates the development of an online two-dimensional liquid chromatography (2D-LC) method where the first dimension column is eluted by a sequence of pulses of increasing eluotropic strength generated by the LC pumps (pulsed-elution 2D-LC). Between the pulses, the first dimension is kept in a no-elution state using low eluent strength. The eluate from the first dimension is actively modulated using trap columns and subsequently analyzed in the second dimension. We demonstrate that by tuning the length and eluotropic strength of the pulses, peaks with retention factors in water, kw, above 150 can be manipulated to elute in 3-4 pulses. The no-elution state can be kept for 1-10 min with only minor changes as to which and how many pulses the peaks elute in. Pulsed-elution 2D-LC combined with active modulation tackles three of the main challenges encountered in 2D-LC and specifically online comprehensive 2D-LC: undersampling, difficulties in refocusing, and lack of flexibility in the selection of column dimensions and flow rates because the two dimensions constrain each other. The pulsed-elution 2D-LC was applied for the analysis of a basic fraction of vacuum gas oil. Peak capacity was 4018 for a 540 min analysis and 4610 for a 1040 min analysis.
KW - Journal Article
U2 - 10.1021/acs.analchem.7b00758
DO - 10.1021/acs.analchem.7b00758
M3 - Journal article
C2 - 28758730
VL - 89
SP - 8723
EP - 8730
JO - Industrial And Engineering Chemistry Analytical Edition
JF - Industrial And Engineering Chemistry Analytical Edition
SN - 0003-2700
IS - 17
ER -
ID: 182933286