Cloning and characterization of a pathogen-induced chitinase in Brassica napus

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Cloning and characterization of a pathogen-induced chitinase in Brassica napus. / Rasmussen, Ulla; Bojsen, Kirsten; Collinge, David B.

In: Plant Molecular Biology, Vol. 20, No. 2, 01.10.1992, p. 277-287.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Rasmussen, U, Bojsen, K & Collinge, DB 1992, 'Cloning and characterization of a pathogen-induced chitinase in Brassica napus', Plant Molecular Biology, vol. 20, no. 2, pp. 277-287. https://doi.org/10.1007/BF00014495

APA

Rasmussen, U., Bojsen, K., & Collinge, D. B. (1992). Cloning and characterization of a pathogen-induced chitinase in Brassica napus. Plant Molecular Biology, 20(2), 277-287. https://doi.org/10.1007/BF00014495

Vancouver

Rasmussen U, Bojsen K, Collinge DB. Cloning and characterization of a pathogen-induced chitinase in Brassica napus. Plant Molecular Biology. 1992 Oct 1;20(2):277-287. https://doi.org/10.1007/BF00014495

Author

Rasmussen, Ulla ; Bojsen, Kirsten ; Collinge, David B. / Cloning and characterization of a pathogen-induced chitinase in Brassica napus. In: Plant Molecular Biology. 1992 ; Vol. 20, No. 2. pp. 277-287.

Bibtex

@article{d5c74d53a38842b6b78a25b9725517ac,
title = "Cloning and characterization of a pathogen-induced chitinase in Brassica napus",
abstract = "A chitinase cDNA clone from rapeseed (Brassica napus L. ssp. Oleifera) was isolated. The cDNA clone, ChB4, represents a previously purified and characterized basic chitinase isozyme. The longest open reading frame in ChB4 encodes a polypeptide of 268 amino acids. This polypeptide consists of a 24 amino acid N-terminal signal peptide, a cysteine-rich domain and a catalytic domain. The primary structure of the mature ChB4 shows a low degree of identity with class I and II chitinases, 43-48% and 35% respectively. In contrast, ChB4 shows 62% identity to a basic sugar-beet chitinase and 63% identity to an acidic chitinase from bean. The expression of chitinase messenger RNA (mRNA) in response to infection with Phoma lingam (Tode ex. Fr.) Desm. was examined by northern hybridization and scintilation counting. A differential induction was seen between resistant and susceptible cultivars where 3-fold higher chitinase transcript levels were estimated one day after inoculation in resistant as compared to susceptible cultivar. This difference diminished eight days after inoculation. Southern hybridization analysis indicates that the chitinase is encoded by a small family of genes.",
keywords = "cDNA cloning, Leptosphaeria maculans, mRNA induction, PCR, Phoma lingam, plant defence",
author = "Ulla Rasmussen and Kirsten Bojsen and Collinge, {David B.}",
year = "1992",
month = oct,
day = "1",
doi = "10.1007/BF00014495",
language = "English",
volume = "20",
pages = "277--287",
journal = "Plant Molecular Biology",
issn = "0167-4412",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - Cloning and characterization of a pathogen-induced chitinase in Brassica napus

AU - Rasmussen, Ulla

AU - Bojsen, Kirsten

AU - Collinge, David B.

PY - 1992/10/1

Y1 - 1992/10/1

N2 - A chitinase cDNA clone from rapeseed (Brassica napus L. ssp. Oleifera) was isolated. The cDNA clone, ChB4, represents a previously purified and characterized basic chitinase isozyme. The longest open reading frame in ChB4 encodes a polypeptide of 268 amino acids. This polypeptide consists of a 24 amino acid N-terminal signal peptide, a cysteine-rich domain and a catalytic domain. The primary structure of the mature ChB4 shows a low degree of identity with class I and II chitinases, 43-48% and 35% respectively. In contrast, ChB4 shows 62% identity to a basic sugar-beet chitinase and 63% identity to an acidic chitinase from bean. The expression of chitinase messenger RNA (mRNA) in response to infection with Phoma lingam (Tode ex. Fr.) Desm. was examined by northern hybridization and scintilation counting. A differential induction was seen between resistant and susceptible cultivars where 3-fold higher chitinase transcript levels were estimated one day after inoculation in resistant as compared to susceptible cultivar. This difference diminished eight days after inoculation. Southern hybridization analysis indicates that the chitinase is encoded by a small family of genes.

AB - A chitinase cDNA clone from rapeseed (Brassica napus L. ssp. Oleifera) was isolated. The cDNA clone, ChB4, represents a previously purified and characterized basic chitinase isozyme. The longest open reading frame in ChB4 encodes a polypeptide of 268 amino acids. This polypeptide consists of a 24 amino acid N-terminal signal peptide, a cysteine-rich domain and a catalytic domain. The primary structure of the mature ChB4 shows a low degree of identity with class I and II chitinases, 43-48% and 35% respectively. In contrast, ChB4 shows 62% identity to a basic sugar-beet chitinase and 63% identity to an acidic chitinase from bean. The expression of chitinase messenger RNA (mRNA) in response to infection with Phoma lingam (Tode ex. Fr.) Desm. was examined by northern hybridization and scintilation counting. A differential induction was seen between resistant and susceptible cultivars where 3-fold higher chitinase transcript levels were estimated one day after inoculation in resistant as compared to susceptible cultivar. This difference diminished eight days after inoculation. Southern hybridization analysis indicates that the chitinase is encoded by a small family of genes.

KW - cDNA cloning

KW - Leptosphaeria maculans

KW - mRNA induction

KW - PCR

KW - Phoma lingam

KW - plant defence

UR - http://www.scopus.com/inward/record.url?scp=0026933301&partnerID=8YFLogxK

U2 - 10.1007/BF00014495

DO - 10.1007/BF00014495

M3 - Journal article

C2 - 1391771

AN - SCOPUS:0026933301

VL - 20

SP - 277

EP - 287

JO - Plant Molecular Biology

JF - Plant Molecular Biology

SN - 0167-4412

IS - 2

ER -

ID: 201510542