A plant plasma membrane Ca2+ pump is required for normal pollen tube growth and fertilization.
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A plant plasma membrane Ca2+ pump is required for normal pollen tube growth and fertilization. / Schiøtt, Morten; Romanowsky, Shawn M; Bækgaard, Lone; Jakobsen, Mia Kyed; Palmgren, Michael; Harper, Jeffrey F.
In: Proceedings of the National Academy of Science of the United States of America, Vol. 101, No. 25, 2004, p. 9502-7.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - A plant plasma membrane Ca2+ pump is required for normal pollen tube growth and fertilization.
AU - Schiøtt, Morten
AU - Romanowsky, Shawn M
AU - Bækgaard, Lone
AU - Jakobsen, Mia Kyed
AU - Palmgren, Michael
AU - Harper, Jeffrey F
N1 - Keywords: Arabidopsis; Calcium Signaling; Calcium-Transporting ATPases; Cell Membrane; Fertilization; Genome, Plant; Plasmids; Pollen
PY - 2004
Y1 - 2004
N2 - Ca(2+) signals are thought to play important roles in plant growth and development, including key aspects of pollen tube growth and fertilization. The dynamics of a Ca(2+) signal are largely controlled by influx (through channels) and efflux (through pumps and antiporters). The Arabidopsis genome encodes 14 Ca(2+) pumps, 10 of which belong to a family of autoinhibited Ca(2+) ATPases (ACA) that are predicted to be activated by Ca(2+)/calmodulin. Here, we show that isoform ACA9 is expressed primarily in pollen and localized to the plasma membrane. Three independent T-DNA [portion of the Ti (tumor-inducing) plasmid that is transferred to plant cells] gene disruptions of ACA9 were found to result in partial male sterility. Complementation was observed by using a ACA9-yellow fluorescence protein (YFP) fusion that displayed plasma membrane localization. Mutant aca9 pollen displayed a reduced growth potential and a high frequency of aborted fertilization, resulting in a >80% reduction in seed set. These findings identify a plasma membrane Ca(2+) transporter as a key regulator of pollen development and fertilization in flowering plants.
AB - Ca(2+) signals are thought to play important roles in plant growth and development, including key aspects of pollen tube growth and fertilization. The dynamics of a Ca(2+) signal are largely controlled by influx (through channels) and efflux (through pumps and antiporters). The Arabidopsis genome encodes 14 Ca(2+) pumps, 10 of which belong to a family of autoinhibited Ca(2+) ATPases (ACA) that are predicted to be activated by Ca(2+)/calmodulin. Here, we show that isoform ACA9 is expressed primarily in pollen and localized to the plasma membrane. Three independent T-DNA [portion of the Ti (tumor-inducing) plasmid that is transferred to plant cells] gene disruptions of ACA9 were found to result in partial male sterility. Complementation was observed by using a ACA9-yellow fluorescence protein (YFP) fusion that displayed plasma membrane localization. Mutant aca9 pollen displayed a reduced growth potential and a high frequency of aborted fertilization, resulting in a >80% reduction in seed set. These findings identify a plasma membrane Ca(2+) transporter as a key regulator of pollen development and fertilization in flowering plants.
U2 - 10.1073/pnas.0401542101
DO - 10.1073/pnas.0401542101
M3 - Journal article
C2 - 15197266
VL - 101
SP - 9502
EP - 9507
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 25
ER -
ID: 3500897