PhD summer school: BIO Advanced Live Cell Imaging

Fibroblast (skin cells) labeled with fluorescent dyes

See the Summer School Poster

Content

The summer course for experienced microscopists comprises theory and show-cases on selected topics, such as Fluorescence Lifetime, Super Resolution and High Throughput Microscopy as well as high speed live and spectral imaging. Internationally outstanding experts will give key notes on these topics. It will be possible for the students to bring their own specimens, if suitable for the chosen techniques and agreed upon (please indicate your interest at the latest end of May). Part of the summer course is the annual meeting of the Danish Bioimaging network with opportunity to present posters or talks.​​

​​Aim and content

Friday, June 21

Morning:

Introduction (Alexander Schulz)

Theory 1: Microscopy basics, theory of fluorescence (Alexander Schulz)

4x 10min participant presentations

Theory 2: Molecular biology and microscopy, Visualization of protein-protein interaction with Bimolecular fluorescence complementation (Michael Lisby) 

Afternoon:

4x 10min participant presentations

Theory 3: Cell physiology, live-cell imaging (Jakob Grunnet Knudsen)

4x 10min participant presentations

Theory 4: Introduction to advanced optical instruments, super-resolution and spinning-disc microscopy (Sebastian Kjeldgaard-Nintemann and Nynne Christensen)

4x 10min student presentations

Evening:

Reception

Monday, June 24

Danish Bioimaging network meeting at Panum, scientific talks and workshops, Conference dinner. 

Please sign up here: DBI Scientific Meeting | Sign up (ku.dk)

Tuesday, June 25

Danish Bioimaging network meeting at Panum, scientific talks + workshops

Wednesday June 26

Morning:

1. Guest lecture by Roland Nitschke: High resolution microscopy

2. Guest lecture by Dorus Gadella: FLIM/FRET.

Afternoon: 

Show-case: High content screening microscopy (Nynne Christensen)

Showcase: Confocal imaging, Fluorescence lifetime imaging (Sebastian Kjeldgaard-Nintemann),

Thursday June 27

Morning:

1. Guest lecture by Lecture by Julia Fernandez-Rodrigue: Correlated Multimodal Imaging

2. Guest lecture to be determined.

Afternoon:

Hands-on: Spinning disk confocal imaging (Guillermo Sergio Moreno Pescador) Hands-on: Perfussion setup, live imaging of tissue (Jakob Grunnet Knudsen). 

Discussion and course conclusion.​

Formal requirem​​ents

Students interested in the course are requested to apply with a short motivation and, when accepted, to give a short presentation of their research project and make an oral presentation or a poster at the DBN meeting

Learning outcome

Participants will learn the theory behind advanced microscopy techniques and how to utilize their full potential from internationally outstanding experts that will be part of the course. It will be possible for the students to bring their own specimens. 
The course will enable the participants to design bioimaging experiments for their own PhD project. 

Target group

PhD students within the biological, biophysical, environmental, life, and medical sciences.

Lecturers

Guest lecturer:
Julia Fernandez-Rodriguez, University of Gothenburg, will give a talk on Correlated Multimodal Imaging and participate in the hands-on laboratory exercise.

Dorus Gadella, University of Amsterdam, will give a talk on fluorescence life-time imaging microscopy and participate in the hands-on laboratory exercise.

Roland Nitschke, University of Freiburg, will give a talk on high resolution microscopy and participate in the hands-on laboratory exercise

From UCPH-CAB:
Professor Michael Lisby, mlisby@bio.ku.dk
Professor Alexander Schulz, als@plen.ku.dk
Assistant Professor Jakob Grunnet Knudsen, jgknudsen@bio.ku.dk

Nynne Christensen, nmchristensen@bio.ku.dk
Sebastian Kjeldgaard-Nintemann, seni@plen.ku.dk​

Remarks

Signing up:

If you would like to participate please:
1) Register online by clicking on "Apply" and
2) Send an e-mail to Nynne M Christensen stating your motivations (10 lines) and which microscopy methods you have used before (2-3 lines).

The course takes place at the Center for Advanced Imaging with locations at the North Campus and the Frederiksberg Campus.

Find more details and apply here