Widespread biosynthesis of 16-carbon terpenoids in bacteria

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Widespread biosynthesis of 16-carbon terpenoids in bacteria. / Duan, Yao Tao; Koutsaviti, Aikaterini; Harizani, Maria; Ignea, Codruta; Roussis, Vassilios; Zhao, Yong; Ioannou, Efstathia; Kampranis, Sotirios C.

I: Nature Chemical Biology, Bind 19, 2023, s. 1532-1539.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Duan, YT, Koutsaviti, A, Harizani, M, Ignea, C, Roussis, V, Zhao, Y, Ioannou, E & Kampranis, SC 2023, 'Widespread biosynthesis of 16-carbon terpenoids in bacteria', Nature Chemical Biology, bind 19, s. 1532-1539. https://doi.org/10.1038/s41589-023-01445-9

APA

Duan, Y. T., Koutsaviti, A., Harizani, M., Ignea, C., Roussis, V., Zhao, Y., Ioannou, E., & Kampranis, S. C. (2023). Widespread biosynthesis of 16-carbon terpenoids in bacteria. Nature Chemical Biology, 19, 1532-1539. https://doi.org/10.1038/s41589-023-01445-9

Vancouver

Duan YT, Koutsaviti A, Harizani M, Ignea C, Roussis V, Zhao Y o.a. Widespread biosynthesis of 16-carbon terpenoids in bacteria. Nature Chemical Biology. 2023;19:1532-1539. https://doi.org/10.1038/s41589-023-01445-9

Author

Duan, Yao Tao ; Koutsaviti, Aikaterini ; Harizani, Maria ; Ignea, Codruta ; Roussis, Vassilios ; Zhao, Yong ; Ioannou, Efstathia ; Kampranis, Sotirios C. / Widespread biosynthesis of 16-carbon terpenoids in bacteria. I: Nature Chemical Biology. 2023 ; Bind 19. s. 1532-1539.

Bibtex

@article{e412ea931d374e46ad78395d291eb969,

title = "Widespread biosynthesis of 16-carbon terpenoids in bacteria",

abstract = "Terpenoids are the most diverse group of specialized metabolites with numerous applications. Their biosynthesis is based on the five-carbon isoprene building block and, as a result, almost all terpenoids isolated to date are based on backbones that contain multiples of five carbon atoms. Intrigued by the discovery of an unusual bacterial terpenoid with a 16-carbon skeleton, here we investigate whether the biosynthesis of 16-carbon terpenoids is more widespread than this single example. We mine bacterial genomic information and identify potential C16 biosynthetic clusters in more than 700 sequenced genomes. We study selected clusters using a yeast synthetic biology platform and reveal that the encoded synthases produce at least 47 different noncanonical terpenoids. By thorough chemical analysis, we explain the structures of 13 C16 metabolites, most of which possess intricate highly strained bi- and tricyclic backbones. Our results unveil the existence of an extensive class of terpenoids in bacteria. [Figure not available: see fulltext.].",

author = "Duan, {Yao Tao} and Aikaterini Koutsaviti and Maria Harizani and Codruta Ignea and Vassilios Roussis and Yong Zhao and Efstathia Ioannou and Kampranis, {Sotirios C.}",

note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive licence to Springer Nature America, Inc.",

year = "2023",

doi = "10.1038/s41589-023-01445-9",

language = "English",

volume = "19",

pages = "1532--1539",

journal = "Nature Chemical Biology",

issn = "1552-4450",

publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Widespread biosynthesis of 16-carbon terpenoids in bacteria

AU - Duan, Yao Tao

AU - Koutsaviti, Aikaterini

AU - Harizani, Maria

AU - Ignea, Codruta

AU - Roussis, Vassilios

AU - Zhao, Yong

AU - Ioannou, Efstathia

AU - Kampranis, Sotirios C.

PY - 2023

Y1 - 2023

N2 - Terpenoids are the most diverse group of specialized metabolites with numerous applications. Their biosynthesis is based on the five-carbon isoprene building block and, as a result, almost all terpenoids isolated to date are based on backbones that contain multiples of five carbon atoms. Intrigued by the discovery of an unusual bacterial terpenoid with a 16-carbon skeleton, here we investigate whether the biosynthesis of 16-carbon terpenoids is more widespread than this single example. We mine bacterial genomic information and identify potential C16 biosynthetic clusters in more than 700 sequenced genomes. We study selected clusters using a yeast synthetic biology platform and reveal that the encoded synthases produce at least 47 different noncanonical terpenoids. By thorough chemical analysis, we explain the structures of 13 C16 metabolites, most of which possess intricate highly strained bi- and tricyclic backbones. Our results unveil the existence of an extensive class of terpenoids in bacteria. [Figure not available: see fulltext.].

AB - Terpenoids are the most diverse group of specialized metabolites with numerous applications. Their biosynthesis is based on the five-carbon isoprene building block and, as a result, almost all terpenoids isolated to date are based on backbones that contain multiples of five carbon atoms. Intrigued by the discovery of an unusual bacterial terpenoid with a 16-carbon skeleton, here we investigate whether the biosynthesis of 16-carbon terpenoids is more widespread than this single example. We mine bacterial genomic information and identify potential C16 biosynthetic clusters in more than 700 sequenced genomes. We study selected clusters using a yeast synthetic biology platform and reveal that the encoded synthases produce at least 47 different noncanonical terpenoids. By thorough chemical analysis, we explain the structures of 13 C16 metabolites, most of which possess intricate highly strained bi- and tricyclic backbones. Our results unveil the existence of an extensive class of terpenoids in bacteria. [Figure not available: see fulltext.].

U2 - 10.1038/s41589-023-01445-9

DO - 10.1038/s41589-023-01445-9

M3 - Journal article

C2 - 37828399

AN - SCOPUS:85174015748

VL - 19

SP - 1532

EP - 1539

JO - Nature Chemical Biology

JF - Nature Chemical Biology

SN - 1552-4450

ER -

ID: 371374679