Unveiling plant defense arsenal: metabolic strategies in Brassica oleracea during black rot disease

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Alterations in plant metabolism play a key role in the complex plant–pathogen interactions. However, there is still a lack of knowledgeabout the connection between changes in primary and specialized metabolism and the plant defense against diseases that impact crops.Thus, we aim to study the metabolic reprograming in Brassica oleracea plants upon infection by Xanthomonas campestris pv. campestris (Xcc).To accomplish this, we utilized a combination of untargeted and targeted metabolomics, through UPLC-Q-TOF-MS/MS and 1H-NMR, intwo crop lines differing in resistance that were evaluated at two- and four-week intervals following inoculation (T1 and T2, respectively).Besides, to depict the physiological status of the plant during infection, enzymatic activities related to the carbohydrate pathwayand oxidative stress were studied. Our results revealed different temporal dynamics in the responses of the susceptible vs. resistantcrops lines. Resistant B. oleracea line suppresses carbohydrate metabolism contributing to limit nutrient supplies to the bacterium andprioritizes the induction of defensive compounds such as indolic glucosinolates, salicylic acid, phenylpropanoids and phytoalexinsprecursors at early infection stages. In contrast, the susceptible line invests in carbohydrate metabolism, including enzymatic activitiesrelated to the hexoses turnover, and activates defense signaling related to reactive oxygen species. Thus, each line triggers a differentmetabolic strategy that will affect how the plant overcomes the disease in terms of resistance and growth. This work provides firstinsights of a fine-tuned metabolic regulation during Xcc infection in B. oleracea that will contribute to develop new strategies for plantdisease management.
OriginalsprogEngelsk
Artikelnummeruhad204
TidsskriftHorticulture Research
Vol/bind10
Udgave nummer11
Antal sider11
ISSN2662-6810
DOI
StatusUdgivet - 2023

Bibliografisk note

Funding Information:
This research was supported by the research projects PID2021-126472OB-I00 and RTI2018-094650-J-100 of the Ministry of Science and Innovation, the Government of Spain. Carmen Vega-Álvarez acknowledges a PFI fellowship from the Spanish Ministry of Science and Innovation. Marta Francisco acknowledges the Ramón y Cajal Research Program (RYC2019-027834-I) through the MCIN/AEI/10.13039/501100011033 and “ESF Investing in your future”. Thomas Roitsch would like to acknowledge funding by the Ministry of Education, Youth and Sports of Czech Republic within the National Sustainability Programme I (NPU I), grant number LO1415. The authors want to thank Juan Carlos Fernández, Victor Rodriguez, Rogelio Santiago, and Ana Carballeda at MBG and to Mohsina Ferdous and Chandana Pandey at the University of Copenhagen.

Funding Information:
This research was supported by the research projects PID2021-126472OB-I00 and RTI2018-094650-J-100 of the Ministry of Science and Innovation, the Government of Spain. Carmen Vega-Álvarez acknowledges a PFI fellowship from the Spanish Ministry of Science and Innovation. Marta Francisco acknowledges the Ramón y Cajal Research Program (RYC2019-027834-I) through the MCIN/AEI/10.13039/501100011033 and “ESF Investing in your future”. Thomas Roitsch would like to acknowledge funding by the Ministry of Education, Youth and Sports of Czech Republic within the National Sustainability Programme I (NPU I), grant number LO1415. The authors want to thank Juan Carlos Fernández, Victor Rodriguez, Rogelio Santiago, and Ana Carballeda at MBG and to Mohsina Ferdous and Chandana Pandey at the University of Copenhagen.

Publisher Copyright:
© 2023 The Author(s). Published by Oxford University Press on behalf of Nanjing Agricultural University.

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