SWEET11b transports both sugar and cytokinin in developing barley grains

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Standard

SWEET11b transports both sugar and cytokinin in developing barley grains. / Radchuk, Volodymyr; Belew, Zeinu M; Gündel, Andre; Mayer, Simon; Hilo, Alexander; Hensel, Goetz; Sharma, Rajiv; Neumann, Kerstin; Ortleb, Stefan; Wagner, Steffen; Muszynska, Aleksandra; Crocoll, Christoph; Xu, Deyang; Hoffie, Iris; Kumlehn, Jochen; Fuchs, Joerg; Peleke, Fritz F; Szymanski, Jedrzej J; Rolletschek, Hardy; Nour-Eldin, Hussam H; Borisjuk, Ljudmilla.

I: The Plant Cell, Bind 35, Nr. 6, 2023, s. 2186-2207.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Radchuk, V, Belew, ZM, Gündel, A, Mayer, S, Hilo, A, Hensel, G, Sharma, R, Neumann, K, Ortleb, S, Wagner, S, Muszynska, A, Crocoll, C, Xu, D, Hoffie, I, Kumlehn, J, Fuchs, J, Peleke, FF, Szymanski, JJ, Rolletschek, H, Nour-Eldin, HH & Borisjuk, L 2023, 'SWEET11b transports both sugar and cytokinin in developing barley grains', The Plant Cell, bind 35, nr. 6, s. 2186-2207. https://doi.org/10.1093/plcell/koad055

APA

Radchuk, V., Belew, Z. M., Gündel, A., Mayer, S., Hilo, A., Hensel, G., Sharma, R., Neumann, K., Ortleb, S., Wagner, S., Muszynska, A., Crocoll, C., Xu, D., Hoffie, I., Kumlehn, J., Fuchs, J., Peleke, F. F., Szymanski, J. J., Rolletschek, H., ... Borisjuk, L. (2023). SWEET11b transports both sugar and cytokinin in developing barley grains. The Plant Cell, 35(6), 2186-2207. https://doi.org/10.1093/plcell/koad055

Vancouver

Radchuk V, Belew ZM, Gündel A, Mayer S, Hilo A, Hensel G o.a. SWEET11b transports both sugar and cytokinin in developing barley grains. The Plant Cell. 2023;35(6):2186-2207. https://doi.org/10.1093/plcell/koad055

Author

Radchuk, Volodymyr ; Belew, Zeinu M ; Gündel, Andre ; Mayer, Simon ; Hilo, Alexander ; Hensel, Goetz ; Sharma, Rajiv ; Neumann, Kerstin ; Ortleb, Stefan ; Wagner, Steffen ; Muszynska, Aleksandra ; Crocoll, Christoph ; Xu, Deyang ; Hoffie, Iris ; Kumlehn, Jochen ; Fuchs, Joerg ; Peleke, Fritz F ; Szymanski, Jedrzej J ; Rolletschek, Hardy ; Nour-Eldin, Hussam H ; Borisjuk, Ljudmilla. / SWEET11b transports both sugar and cytokinin in developing barley grains. I: The Plant Cell. 2023 ; Bind 35, Nr. 6. s. 2186-2207.

Bibtex

@article{95a218ee6a25495497924fa5de8227b3,

title = "SWEET11b transports both sugar and cytokinin in developing barley grains",

abstract = "Even though SWEETs (Sugars Will Eventually be Exported Transporters) have been found in every sequenced plant genome, a comprehensive understanding of their functionality is lacking. In this study, we focused on the SWEET family of barley (Hordeum vulgare). A radiotracer assay revealed that expressing HvSWEET11b in African clawed frog (Xenopus laevis) oocytes facilitated the bidirectional transfer of not just sucrose and glucose, but also cytokinin. Barley plants harboring a loss-of-function mutation of HvSWEET11b could not set viable grains, while the distribution of sucrose and cytokinin was altered in developing grains of plants in which the gene was knocked down. Sucrose allocation within transgenic grains was disrupted, which is consistent with the changes to the cytokinin gradient across grains, as visualized by magnetic resonance imaging and Fourier transform infrared spectroscopy microimaging. Decreasing HvSWEET11b expression in developing grains reduced overall grain size, sink strength, the number of endopolyploid endosperm cells, and the contents of starch and protein. The control exerted by HvSWEET11b over sugars and cytokinins likely predetermines their synergy, resulting in adjustments to the grain's biochemistry and transcriptome.",

author = "Volodymyr Radchuk and Belew, {Zeinu M} and Andre G{\"u}ndel and Simon Mayer and Alexander Hilo and Goetz Hensel and Rajiv Sharma and Kerstin Neumann and Stefan Ortleb and Steffen Wagner and Aleksandra Muszynska and Christoph Crocoll and Deyang Xu and Iris Hoffie and Jochen Kumlehn and Joerg Fuchs and Peleke, {Fritz F} and Szymanski, {Jedrzej J} and Hardy Rolletschek and Nour-Eldin, {Hussam H} and Ljudmilla Borisjuk",

note = "{\textcopyright} The Author(s) 2023. Published by Oxford University Press on behalf of American Society of Plant Biologists.",

year = "2023",

doi = "10.1093/plcell/koad055",

language = "English",

volume = "35",

pages = "2186--2207",

journal = "The Plant Cell",

issn = "1040-4651",

publisher = "American Society of Plant Biologists",

number = "6",

}

RIS

TY - JOUR

T1 - SWEET11b transports both sugar and cytokinin in developing barley grains

AU - Radchuk, Volodymyr

AU - Belew, Zeinu M

AU - Gündel, Andre

AU - Mayer, Simon

AU - Hilo, Alexander

AU - Hensel, Goetz

AU - Sharma, Rajiv

AU - Neumann, Kerstin

AU - Ortleb, Stefan

AU - Wagner, Steffen

AU - Muszynska, Aleksandra

AU - Crocoll, Christoph

AU - Xu, Deyang

AU - Hoffie, Iris

AU - Kumlehn, Jochen

AU - Fuchs, Joerg

AU - Peleke, Fritz F

AU - Szymanski, Jedrzej J

AU - Rolletschek, Hardy

AU - Nour-Eldin, Hussam H

AU - Borisjuk, Ljudmilla

PY - 2023

Y1 - 2023

N2 - Even though SWEETs (Sugars Will Eventually be Exported Transporters) have been found in every sequenced plant genome, a comprehensive understanding of their functionality is lacking. In this study, we focused on the SWEET family of barley (Hordeum vulgare). A radiotracer assay revealed that expressing HvSWEET11b in African clawed frog (Xenopus laevis) oocytes facilitated the bidirectional transfer of not just sucrose and glucose, but also cytokinin. Barley plants harboring a loss-of-function mutation of HvSWEET11b could not set viable grains, while the distribution of sucrose and cytokinin was altered in developing grains of plants in which the gene was knocked down. Sucrose allocation within transgenic grains was disrupted, which is consistent with the changes to the cytokinin gradient across grains, as visualized by magnetic resonance imaging and Fourier transform infrared spectroscopy microimaging. Decreasing HvSWEET11b expression in developing grains reduced overall grain size, sink strength, the number of endopolyploid endosperm cells, and the contents of starch and protein. The control exerted by HvSWEET11b over sugars and cytokinins likely predetermines their synergy, resulting in adjustments to the grain's biochemistry and transcriptome.

AB - Even though SWEETs (Sugars Will Eventually be Exported Transporters) have been found in every sequenced plant genome, a comprehensive understanding of their functionality is lacking. In this study, we focused on the SWEET family of barley (Hordeum vulgare). A radiotracer assay revealed that expressing HvSWEET11b in African clawed frog (Xenopus laevis) oocytes facilitated the bidirectional transfer of not just sucrose and glucose, but also cytokinin. Barley plants harboring a loss-of-function mutation of HvSWEET11b could not set viable grains, while the distribution of sucrose and cytokinin was altered in developing grains of plants in which the gene was knocked down. Sucrose allocation within transgenic grains was disrupted, which is consistent with the changes to the cytokinin gradient across grains, as visualized by magnetic resonance imaging and Fourier transform infrared spectroscopy microimaging. Decreasing HvSWEET11b expression in developing grains reduced overall grain size, sink strength, the number of endopolyploid endosperm cells, and the contents of starch and protein. The control exerted by HvSWEET11b over sugars and cytokinins likely predetermines their synergy, resulting in adjustments to the grain's biochemistry and transcriptome.

U2 - 10.1093/plcell/koad055

DO - 10.1093/plcell/koad055

M3 - Journal article

C2 - 36857316

VL - 35

SP - 2186

EP - 2207

JO - The Plant Cell

JF - The Plant Cell

SN - 1040-4651

IS - 6

ER -

ID: 338504265