TY - JOUR

T1 - Proteomic dissection of the Arabidopsis Golgi and trans-Golgi network

AU - Parsons, Harriet Tempé

AU - Drakakaki, Georgia

AU - Heazlewood, Joshua L.

N1 - OA

PY - 2013

Y1 - 2013

N2 - The plant Golgi apparatus and trans-Golgi network are major endomembrane trafficking hubs within the plant cell and are involved in a diverse and vital series of functions to maintain plant growth and development. Recently, a series of disparate technical approaches have been used to isolate and characterize components of these complex organelles by mass spectrometry in the model plant Arabidopsis thaliana. Collectively, these studies have increased the number of Golgi and vesicular localized proteins identified by mass spectrometry to nearly 500 proteins. We have sought to provide a brief overview of these technical approaches and bring the datasets together to examine how they can reveal insights into the secretory pathway.

AB - The plant Golgi apparatus and trans-Golgi network are major endomembrane trafficking hubs within the plant cell and are involved in a diverse and vital series of functions to maintain plant growth and development. Recently, a series of disparate technical approaches have been used to isolate and characterize components of these complex organelles by mass spectrometry in the model plant Arabidopsis thaliana. Collectively, these studies have increased the number of Golgi and vesicular localized proteins identified by mass spectrometry to nearly 500 proteins. We have sought to provide a brief overview of these technical approaches and bring the datasets together to examine how they can reveal insights into the secretory pathway.

KW - Golgi

KW - trans-Golgi network

KW - proteomics

KW - LOPIT

KW - free-flow electrophoresis

KW - Arabidopsis

KW - SYP61

U2 - 10.3389/fpls.2012.00298

DO - 10.3389/fpls.2012.00298

M3 - Journal article

C2 - 23316206

VL - 3

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

ER -