Multimodal correlative imaging and modelling of phosphorus uptake from soil by hyphae of mycorrhizal fungi
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Phosphorus (P) is essential for plant growth. Arbuscular mycorrhizal fungi (AMF) aid its uptake by acquiring P from sources distant from roots in return for carbon. Little is known about how AMF colonise soil pore-space, and models of AMF-enhanced P-uptake are poorly validated. We used synchrotron X-ray computed tomography to visualize mycorrhizas in soil and synchrotron X-ray fluorescence/X-ray absorption near edge structure (XRF/XANES) elemental mapping for P, sulphur (S) and aluminium (Al) in combination with modelling. We found that AMF inoculation had a suppressive effect on colonisation by other soil fungi and identified differences in structure and growth rate between hyphae of AMF and nonmycorrhizal fungi. Our results showed that AMF co-locate with areas of high P and low Al, and preferentially associate with organic-type P species over Al-rich inorganic P. We discovered that AMF avoid Al-rich areas as a source of P. Sulphur-rich regions were found to be correlated with higher hyphal density and an increased organic-associated P-pool, whilst oxidized S-species were found close to AMF hyphae. Increased S oxidation close to AMF suggested the observed changes were microbiome-related. Our experimentally-validated model led to an estimate of P-uptake by AMF hyphae that is an order of magnitude lower than rates previously estimated – a result with significant implications for the modelling of plant–soil–AMF interactions.
Originalsprog | Engelsk |
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Tidsskrift | New Phytologist |
Vol/bind | 234 |
Udgave nummer | 2 |
Sider (fra-til) | 688-703 |
Antal sider | 16 |
ISSN | 0028-646X |
DOI | |
Status | Udgivet - 2022 |
Bibliografisk note
Funding Information:
AvV, SK, RM, CS, DMF, SR, CP, KW and TR were funded by ERC Consolidator Grant no. 646809‐DIMR. NK, LC and TR were funded by BBSRC SARISA BB/L02620/1, SD was funded by BBSRC Case Studentship BB/L502625/1, DMF was funded by an EPSRC PhD studentship, TR was also funded by BBSRC BB/P004180/1, NERC NE/L00237/1, EPSRC EP/M020355/1, and AT and PP were funded by BBSRC grants BB/N013387/1 and BB/R017859/1. We would also like to acknowledge the help of Dr Keith Daily in modelling and Pengrui Cai in sequencing analysis. Finally, we gratefully acknowledge the support of the US Department of Energy through the LANL/LDRD Program and the G. T. Seaborg Institute to AvV. Los Alamos National Laboratory is operated by Triad National Security, LLC, for the National Nuclear Security Administration of the US Department of Energy (contract no. 89233218CNA000001).
Publisher Copyright:
© 2022 The Authors. New Phytologist © 2022 New Phytologist Foundation
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