Clubroot disease, caused by Plasmodiophora brassicae, poses a significant global threat tocruciferous crops. The epidemic area of clubroot disease is expanding rapidly. In response to thispressing issue, there is a compelling need for the development of clubroot disease-resistant radishcultivars. China boasts an extensive array of radish varieties and germplasm resources. However,a comprehensive assessment of their resistance to clubroot has not yet been carried out, therebyimpeding the effective utilization of germplasm and clubroot-resistant breeding. Therefore, it is urgentto systematically evaluate the clubroot resistance of the radish germplasm and identify resistantresources. In this study, clubroot resistance evaluations were conducted on 268 excellent radishvarieties derived from 30 provinces in China, as well as seven accessions from Russia, North Korea,France, South Korea, and Germany. The resistance evaluation revealed a diverse range of resistanceindices, with a mean disease index (DI) ranging from 0.6 to 58.5, showing significant disparities inclubroot resistance among these radish resources. A total of six accessions were characterized ashighly resistant to clubroot, and a further 50 accessions were characterized as resistant. The disease-resistant radishes showed diversity in horticultural traits. Provinces in South China contributedsignificantly more resistance germplasm than those of North China. These materials are of greatvalue for both genetic investigation and the crop breeding of clubroot resistance. Furthermore, weemployed a previously established clubroot-resistance-linked SSR marker to analyze the clubroot-resistant resources. The accessions exhibited dissimilar genetic profiles from known clubroot-resistantgermplasm, suggesting their potential status as novel sources of clubroot resistance. Conclusively,these newly identified accessions enriched the genetic diversity within the clubroot-resistant genepool and may contribute to the future cloning of previously undiscovered clubroot-resistant genes.